2012

The effects of callus age, UV irradiation and incubation time on trans-resveratrol production in grapevine callus culture

Bu çalısmada, Kalecik karası Klon 12 (Vitis vinifera L) üzüm çesidine ait kallus kültürlerinde, transresveratrol üretiminin uyarılması üzerine kallus yası, ultraviyole (UV) ısını uygulama ve inkübasyon süresinin etkisi incelenmistir. Kallus eldesi için serada yetisen bir yaslı asmalardan alınan yaprak parçaları (~1 cm2), %2 sakkaroz, % 0.8 agar, 1.0 μM BAP (6-benzilaminopürin) ve 0.1 μM 2, 4-D (2, 4- diklorofenosi-asetik asit) eklenmis Gamborg B-5 ortamında kültüre alınmıstır. İkinci alt kültürden sonra, 12 ve 15 gün yaslı kalluslara, 10 cm uzaklıktan 10 ve 15 dakika süreyle 254 nm dalga boyuna sahip UV ısını uygulanmıstır.Trans-resveratrol üretiminin belirlenmesi amacıyla, Yüksek Basınçlı Sıvı Kromotografisi (HPLC) yönteminden yararlanılmıs, ölçümler 0., 24., 48. ve 72. saatlerde yapılmıstır. Trans-resveratrol konsantrasyonu kontrol kalluslarında, 0.56- 0.96 μg/g fw arasında degismistir. En yüksek trans-resveratrol üretimi, 10 dakika UV ısını uygulaması yapılmıs 12 ve 15 gün yaslı kalluslarda 48. saat ölçümlerinde elde edilmistir (2.42 μg/g fw). Kontrol ve uygulamalarda elde edilen en yüksek trans-resveratrol derisimleri dikkate alındıgında UV ısını uygulamasının Kalecik Karası’nda trans-resveratrol birikimini uyardıgı ve yaklasık 2.5 kat artıs sagladıgı belirlenmistir.

Asma kallus kültürlerinde trans-resveratrol üretimin uyarılması üzerine kallus yaşı, UV ışını uygulama ve inkübasyon sürelerinin etkisi

In this study, the effects of callus age, ultraviolet (UV) irradiation and incubation time were investigated for the induction of trans-resveratrol production in callus cultures of Kalecik karası (Clone 12) Vitis vinifera L. grape cultivar. Part of leaves (~1 cm2) were taken from one year old grapevines grown in greenhouse and then were cultured in Gamborg B-5 media including 2% saccarose, 0.8% agar, 1.0 μM BAP (6- benzylaminopurine) and 0.1 μM 2, 4-D (2, 4-dichlorophenoxy-acetic acid). After the second subculture, 12 and 15 days old callus tissues were exposed to 254 nm UV light at 10 cm distance from the source for 10 and 15 min. Trans-resveratrol was measured at 0, 24, 48 and 72 hours of incubation by using High Pressure Liquid Chromatography (HPLC). Trans-resveratrol concentration of control callus ranged from 0.56 to 0.96 μg g fw-1. The highest trans-resveratrol production was obtained from 12 and 15 days-old callus irradiated for 10 min. at the 48th hours of incubation (2.42 μg g fw-1). Considering the highest value of trans-resveratrol concentration in controls and experiments, it was determined that UV irraditation in Kalecik karası elicitated the trans-resveratrol production by 2.5 times.

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