LABRADOR KÖPEK YAVRULARININ MAMALARINA İLAVE EDİLEN ÇİNKONUN ETKİLERİ

Bu çalışmanınamacı kö pekyavrularımamalarına katı- lançinkonun(Zn)canlıağırlıkdeğişimi,iskeletbü yüme parametreleri ve kimi serum parametrelerine etkisini araştırmaktı.40adetsekizhaftalıkyaştaLabradorRetriever cinsikö pekyavrularıikieşitsayıda(n=20)gruba ayrılarak sekiz hafta sü reyle ticari bir kö pek maması, ineksü tü vehaşlanmışyumurtailebirlikte0(kontrol) veya 100 ppm çinko sülfat (ZnSO4) ilaveli (Uygulama) diyet tü ketmeleri sağlanmıştır. Kö pek yavruları diyetineilave edilen 100 ppm Zn vü cut ağırlığını etkilememiştir (p=0.403).Onemliolmamasınarağmen,Zntakviyelidiyet tü keten kö pek yavruları daha bü yü k vü cut yü ksekliği,vü cutuzunluğ u,gö ğ ü sçevresi,kalçagenişliğive omuz genişliğine sahip olmuşlardır (p=0.097). MamalarınaZnkatılankö pekyavrularınaaitserumlardadü şü k kalsiyum (Ca) (p=0.075), magnezyum (Mg) (p>4.457), bakır (Cu) (p>4.4⁰ 5), ancak daha yüksek kolesterol(p=0.038), glikoz (p=0.010) ve Zn (p=0.017) konsantrasyonlarıtespitedilmiştir.Çalışmasonuçlarına gö rekö pekyavrularımamalarınailaveedilen100ppm Zncanlıağırlığıdeğiştirmezikenö nemsizdeolsaiskelet gelişiminidesteklemiştir.Klinikö nemeaitserumparametrelerimamalarına Zn katılan kö pek yavrularında değişimeuğ ramıştır.

EFFECTS OF SUPPLEMENTAL ZINC TO THE DIET OF LABRADOR PUPPIES

The objective of the present work was to evaluate the effects of supplemental zinc (Zn) to the diet of puppies on live weight changes, skeletal growth parameters, and some of serum metabolites. Forty, eight-week-old Labrador Retriever puppies were divided into two groups (n=20) and were fed a mix of commercial dry dog food, cow milk and boiled egg with (treatment) or without (control) 100 ppm dietary supplemental zinc sulphate (ZnSO4) for 8 weeks. Supplementing 100 ppm Zn to the diet of puppies did not in luence body weights (p>4.847). Although insigni icantly, puppies consuming a diet supplemented with Zn had greater body heights, body lengths, hearth girths, widths of chest, and widths of hip (p=0.097). Puppies on a diet supplemented with Zn had lower calcium (Ca) (p=0.075), magnesium (Mg) (p>4.457), copper (Cu) (p>4.4⁰ 5), but greater cholesterol (p>4.47² ), glucose (p>4.454), and Zn (p>4.45¹ ) serum concentrations compared with puppies fed a diet not supplemented with Zn. The results of the present study showed that supplementing 100 ppm zinc to the diet of Labrador puppies did not change weight gains but, although not greatly, promoted skeletal growth. Serum parameters of clinical importance were in luenced by Zn supplementation.

PDF

___

1. Colombini S. Canine zinc-responsive dermatosis. Vet Clin North Am Small Anim Pract 1999; 29: 13731383.
2. Huber AM, Gershoff SN. Effect of zinc de iciency in rats on insulin release from the pancreas. J Nutr 1973; 103: 17391744.
3. Jou MY, Philipps AF, Lonnerdal B. Maternal zinc de iciency in rats affects growth and glucose metabolism in the offspring by inducing insulin resistance postnatally. J Nutr 2010; 140: 16211627.
4. Byun HR, Kim DK, Koh JY. Obesity and downregulated hypothalamic leptin receptors in male metallothionein-3-null mice. Neurobiol Dis 2011; 44: 125-132.
5. Ege jord L, Petersen AB, Rungby J. Zinc, alpha cells and glucagon secretion. Curr Diabetes Rev 2010; 6: 52-57.
6. Chasapis CT, Loutsidou AC, Spiliopoulou CA, et al. Zinc and human health: an update. Arch Toxicol 2011; 86: 521-534.
7. Kelleher SL, McCormick NH, Velasquez V, et al. Zinc in specialized secretory tissues: roles in the pancreas, prostate, and mammary gland. Adv Nutr 2011; 2: 101-111.
8. MacDonald RS. The role of zinc in growth and cell proliferation. J Nutr 2000; 130: 1500-1558.
9. Henkin, RI. Trace metals in endocrinology. Med Clin North Am 1976; 60: 779-797.
10. Roth HP, Kirchgessner M. Course of concentration changes of growth hormone, IGF-1, insulin and C- peptide in serum, pituitary and liver of zinc- de icient rats. J Anim Phys Anim Nutr 1997; 77: 91 -101.
11. Dorup,I, Flyvbjerg,A, Everts,ME, et al. Role of insulin-like growth factor-1 and growth hormone in growth inhibition induced by magnesium and zinc de iciencies. Br J Nutr 1991; 66: 505-521.
12. Booles D, Burger IH, Whyte AL, et al. Effects of two levels of zinc intake on growth and trace element status in Labrador puppies. J Nutr 1991; 121: 79- 90.
13. Wedekind K, Lowry S. Are organic zinc sources ef icacious in pupppies. J Nutr 1998; 128: 2593- 2595.
14. National Research Council NRC. Nutrient requirements of dogs and cats. National Academy Press, Washington 2006.
15. Lowe J, Wiseman JA. Comparison of the bioavailability of three dietary zinc sources using four different physiological parameters in dogs. J Nutr 1998; 128: 2809-2811.
16. Tepeli C, Çetin O, Inal S, et al. Kangal ve Akbaş ırkı Tü rk çoban kö peklerinde bü yüme ö zellikleri. Turk J Vet Anim Sci 2003; 27: 1011-1018.
17. Palmer J. An Illustrated Guide to Dogs. Salamander Books Ltd, London 1981.
18. SAS Institute. SAS Users Guide: Statistics. SAS Institute Inc., Cary, NC 1996.
19. Hawthorne AJ, Booles D, Nugent PA, et al. Body- weight changes during growth in puppies of different breeds. J Nutr 2004; 134: 20272030.
20. Seo HJ, Cho YE, Kim T, et al. Zinc may increase bone formation through stimulating cell proliferation, alkaline phosphatase activity and collagen synthesis in osteoblastic MC3T3-E1 cells. Nutr Res Pract 2010; 4: 356-361.
21. Richardson DC, Zentek J. Nutrition and osteochondrosis. Vet Clin North Am Small Anim Pract 1998; 28: 115-135.
22. Wu FY, Wu CW. Zinc in DNA replication and transcription. Annu. Rev Nutr 1987; 7: 251-272.
23. Ohlsson C, Bengtsson BA, Isaksson OG, et al. Growth hormone and bone. Endocrinol Rev 1998; 19: 55-79.
24. Lichten LA, Cousins RJ. Mammalian zinc transporters: nutritional and physiologic regulation. Annu Rev Nutr 2009; 29: 153176.
25. Cousins RJ, Liuzzi JP, Lichten LA. Mammalian zinc transport, traf icking, and signals. J Biol Chem 2006; 281: 2408524089.
26. Martin L, Pieper R, Schunter N, et al. Performance, organ zinc concentration, jejunal brush border membrane enzyme activities and mRNA expression in piglets fed with different levels of dietary zinc. Arch Anim Nutr 2013; 67: 248-261.
27. Bonham M, OConnor JM, McAnena LB, et al. Zinc supplementation has no effect on lipoprotein metabolism, hemostasis, and putative indices of copper status in healthy men. Biol Trace Elem Res 2003; 93:75 86.
28. Gatto LM, Samman S. The effect of zinc supplementation on plasma lipids and low-density lipoprotein oxidation in males. Free Rad Biol Med 1995; 19: 517521.
29. Jayawardena R, Ranasinghe P, Galappatthy P, et al. Effects of zinc supplementation on diabetes mellitus: A systematic review and meta-analysis. Diabetol Metab Syndr 2012; 19: 4-13.
30. Taylor CG. Zinc, the pancreas, and diabetes: Insights from rodent studies and future directions. Biometals 2005; 18: 305312.
31. Prost AL, Bloc A, Hussy N. Zinc is both an intracellular and extracellular regulator of KATP channel function. J Physiol 2004; 559: 157167.
32. Maggiore AD, Nelson RW, Dennis J, et al. Ef icacy of protamine zinc recombinant human insulin for controlling hyperglycemia in dogs with diabetes mellitus. J Vet Intern Med 2012; 26: 109-115.
33. Brewer GJ, Dick RD, Schall W, et al. Use of zinc acetate to treat copper toxicosis in dogs. J Am Vet Med Assoc 1992; 201: 564567.
34. Sugawara N, Katakura M, Li D, et al. Role of hepatic coppermetallothionein on liver function of Long- Evans Cinnamon rats with a new mutation causing hereditary hepatitis. Res Commun Chem Pathol Pharmacol 1994; 83: 349358.
35. Walker FC, Kordas K, Stoltzfus RJ, et al. Interactive effects of iron and zinc on biochemical and functional outcomes in supplementation trials. Am J Clin Nutr 2005; 82: 5-12.
36. Yadrick MK, Kenney MA, Winterfeldt EA. Iron, copper, and zinc status: response to supplementation with zinc or zinc and iron in adult females. Am J Clin Nutr 1989; 49: 145150.
37. Lind T, Lonnerdal B, Stenlund H, et al. A community -based randomized controlled trial of iron and zinc supplementation in Indonesian infants: Interactions between iron and zinc. Am J Clin Nutr 2003; 77: 883890.