Öznur ASLAN, Ilknur BEKDİK KARACA, Tuğrul ATALAY

DIAGNOSIS of MYCOPLASMA HAEMOFELIS and CANDIDATUSMYCOPLASMAn HAEMOMINUTUM USING PCR ASSA in CATS

Bu çalışmanın amacı, kedilerde polimeraz zincir reaksiyonu (PCR) ile haemoplasmozisin belirlenmesidir. Çalışmanın materyalini farklı ırk ve cinsiyette (41 dişi ve 43 erkek), ortalama 5.5 yaşlarında (6 ay-10 yaş) 84 kediden toplanan kan ö rnekleri oluşturdu. Kan ö rnekleri hem sitolojik, hem de PCR yö ntemi ile incelendi. PCR reaksiyonu için Mycoplasma haemofelis ve Candidatus Mycoplasma haemominutumun 16S rRNA gen bölgelerinden sırasıyla 170 bp and 193 bp bölgesini çoğaltmak için spesi ik primerler kullanıldı. PCR analizi sonucunda 84 kedinin 8i (%9.52) haemoplazma pozitif olarak belirlendi ve bunlardan 4ü (%4.76) M. haemofelis, 3ü (%3.57) Candidatus M. haemominutum ve bir kedi (%1.19) her iki etken açısından pozitif bulundu. Bilgilerimize gö re bu çalışma ile Tü rkiyede kedilerde M. haemofelisin ilk molekü ler teşhisi yapılmış ve M. haemofelis ve Candidatus M. haemominutum enfeksiyonlarının birlikte tespiti ilk kez bildirilmiştir.

KEDİLERDE PCR İLE MYCOPLASMA HAEMOFELİS VE CANDIDA TUS MYCOPLASMA HEAMOMINUTUM'UN TANISI

In this study, it was aimed to determine haemoplasmosis using polymerase chain reaction (PCR) analysis in cats. The material for this study consisted of blood samples collected from 84 cats of aged average 5.5 years on average (6 months-10 years) and belonged to different strains and sexes (41 females and 43 males). Blood samples were analyzed both cytologically and with PCR. The PCR was performed with the speci ic primer pairs in order to amplify 170 bp and 193 bp region of 16S rRNA gene from Mycoplasma haemofelis and Candidatus Mycoplasma haemominutum, respectively. All positive samples were sequenced in both directions with the ampli ication primers. The PCR analysis showed that 8 of 84 cats (9.52%) were haemoplasma positive and 4 cats (4.76%) were infected with M. haemofelis, 7 (3.57%) were infected with Candidatus M. haemominutum and one cat (5.5³ ± ) was co-infected. To the best of the authors s knowledge, this study reports the irst molecular characterization of M. haemofelis and a coinfection with M. haemofelis and Candidatus M. haemominutum in cats in Turkey

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