Murat Sırrı AKOSMAN, Hasan Hüseyin DEMİREL, Ruhi TÜRKMEN

Farelerde NMDA Reseptör Antagonisti Mk-801 ile Böbrek ve Karaciğerde Oluşan Patolojik Değişikliklere Karşı N-Asetilsisteinin Koruyucu Etkisi

Glutamaterjik sistem sinir sisteminde önemli bir sinaps mekanizmasıdır. N-metil-D-aspartat (NMDA) reseptörleri bu sistemin önemli bir parçasıdır. NMDA reseptörlerinin hipofonksiyonu farelerde kognitif ve motorik sorunlara sebep olmaktadır. MK-801 bu reseptörün oldukça önemli bir blokörüdür. 1mg/kg MK-801’in sub-kronik uygulanması farelerde şizofreni benzeri semptomlara ve beyinde dejenerasyona sebep olmaktadır. Sunulan bu çalışmada MK-801’in 1mg/kg’lık sub-kronik uygulamasının farelerin böbrek ve karaciğerlerinde patolojik etkileri olup olmadığı ve N-asetilsisteinin’in (NAS) bu etkiler karşısında koruyucu etkisinin olup olmadığı araştırıldı. Bu amaçla 24 adet erkek Balb/C faresi alınarak dört eşit gruba bölündü. İlk grup kontrollerden oluştu ve bu gruba %0,9 fizyolojik tuzlu su (10mg/kg) intraperitoneal (i.p.) yolla, ikinci gruba 1mg/kg dozunda MK-801 i.p., üçüncü gruba 1mg/kg MK-801 ile 100mg/kg NAS birlikte i.p ve dördüncü gruba da 100mg/kg NAS i.p. yolla verildi. İlaç uygulamaları 14 gün boyunca sürdü. Sakrifiye edilen farelerin karaciğer ve böbrekleri alınarak bunlara rutin histoloji prosedürü uygulandı. İncelemelerden sonra organlarda histopatolojik değişkilikler ve NAS’ın genelde koruyucu etkisinin olduğu gözlendi. Sonuç olarak NAS’ın böbrek ve karaciğer üzerinde MK-801’le indüklenen histopatolojik değişikliklere karşı önemli bir koruyucu etkisinin olduğu ortaya kondu.

Anahtar Kelimeler:

Böbrek, Fare, Histopatoloji, Karaciğer, MK-801, N-asetilsistein

Protective Effect of N-Acetylcysteine on The Kidney and Liver Pathology Induced by NMDA Receptor Antagonist Mk-801 in Mice

The glutamatergic system and its receptor N-methyl-D-aspartate (NMDA) is an important synapse mechanism in the nervous system. The MK-801 is the blocker of this receptor and according to the literature, the sub-chronic administration of 1mg/kg MK-801 resulted in schizophrenia-like symptoms and degeneration in the mice brain. The aim of the present study was to observe whether this dose of MK-801 has any pathological effect on the liver and kidney tissues and whether N-acetylcysteine (NAC) was a protective effect. For this purpose, the 24 Balb/C male mice were divided into 4 groups, equally. The first group was the control and 0.9% saline (10mg/kg) was intraperitoneally (i.p.) injected. The 1mg/kg MK-801 was i.p. administered to the second group. The third group was i.p. received 1mg/kg MK-801, 100 mg/kg NAC and finally the 100mg/kg NAC i.p. injected to the fourth group. The drug administration was ended on the 14th day. The kidney and the liver of the sacrificed mice were collected and routine histology procedure was applied. The histopathological changes in the organs and NAC’s mostly protective effect were noticed after the observations. In conclusion, NAC has a partially but significantly protective effect against the MK-801 dependent histopathological changes on the kidney and liver tissues.

Keywords:

Histopathology, Kidney, Liver, Mice, MK-801, N-acetylcysteine,

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