Melaminin İnsan Kolon Adenokarsinom Hücreleri Üzerindeki Anti-Proliferatif Etkisi

Melamin, öncelikle plastik dengeleyici ve yangın geciktirici olarak kullanılan organik bir kimyasaldır. Hayvansal yem ve süt ürünlerinin protein içeriğini suni şekilde yükseltmek için kullanılmasıyla bebeklerde böbrek hastalıklarına ve hayvan ölümlerine yol açmıştır. Bu çalışmada, melaminin insan kolon adenokarsinom hücrelerinin (Caco-2) proliferasyonu üzerine etkileri araştırılmıştır. Farklı konsantrasyonlarda çözülmüş melamin Caco-2 hücreleri ile 24 saat inkübe edildi ve MTT testi kullanılarak anti-proliferatif potansiyeli test edildi. Melaminin yarı maksimal inhibisyon konsantrasyonu (IC50), doz-cevap eğrisinden hesaplandı ve hücrelerde 14636 µg/L olarak bulundu. Sonuçlar doza bağımlı olarak melaminin Caco-2 hücrelerinin proliferasyonunu azalttığını ve Caco-2 hücrelerine karşı anti-proliferatif etkiye sahip olduğunu göstermiştir.

Anahtar Kelimeler:

Anti-proliferatif, Caco-2, Melamin, MTT

Anti-Proliferative Effect of Melamine on Human Colon Adenocarcinoma Cells

Melamine is an organic chemical used primarily as a plastics stabilizer and fire retardant. Also, it was used to fraudulently elevate the protein content of animal feed and dairy products, which led to infant kidney diseases and pet deaths. In this study, melamine was studied for its effects on growth in the human colon adenocarcinoma cells (Caco-2). Different concentrations of medium-dissolved melamine were incubated for 24 h with Caco-2 cells and tested for its anti-proliferative potential using MTT assay. Half-maximal inhibitory concentration (IC50) of melamine was calculated from dose-response curve and found to be 14636 µg/L in the cells. The results demonstrated that the dose dependent melamine decreased cell proliferation and direct anti-proliferative effect in Caco-2 cells.

Keywords:

Anti-proliferative, Caco-2, Melamine, MTT,

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