Han GUO, Runqi 1 LIU, Renxu CHANG, Cheng XIA, Chuang XU, Baoyin 1 HUANG, Ping 1 HE, Yuanyuan CHEN, Wei YANG, Ruirui LI, Sansi 1 GAO, Zhihao 1 DONG

Yüksek Yağlı Diyet ve Kolesterolle Beslenen Farelerde Eksojen Fibroblast Büyüme Faktörü-21'in Yangı Üzerine Etkisi

Fibroblast Büyüme Faktörü 21 (FGF21) yağların kullanımında görev alan metabolik bir düzenleyicidir ve yağ metabolizmasında rol oynar. Yüksek yağlı ve kolesterollü diyet ile FGF21'in fizyolojik fonksiyonu arasındaki ilişkiyi araştırmak amacıyla FGF21'in bağışıklık cevap indikatörleri ve kan parametreleri üzerine etkisi incelendi. Çalışmada toplam 24 dişi fare kullanıldı ve bunların 12'si yüksek yağlı diyet (Grup 1) diğer 12'si ise normal diyet (Grup 2) ile beslendi. Otuz gün sonunda iki gruptaki fareler tekrar ikişer gruba bölündü; 1: Yüksek yağlı diyet ile beslenen fareler "model grup" olarak kullanıldı, 2: Normal diyet fareler "kontrol grubu" olarak kullanıldı; 3: Yüksek yağlı diyet ile beslenen ve FGF21 enjekte edilen fareler "model + FGF21 grubu" olarak kullanıldı; 4: normal ile beslenen ve FGF21 enjekte edilen fareler "kontrol + FGF21 grubu" olarak kullanıldı. Fareler 3 gün aralıkla öldürüldü ve karaciğer dokuları incelendi. Kan toplanarak CD3 ve CD19 akışkan sitometresi ve IL-4, IL-6 ve TNF-? ise ELİSA ile analiz edildi. Serum TG, TC, NEFA, LDL-c ve HDL-c seviyeleri otomatik biyokimyasal analizleyici ile ölçüldü. CD3 ve CD19 değişmekle birlikte değişim istatistiki bakımdan anlamlı bulunmadı ve model eksojen enjeksiyon FGF21 grubunda IL-4, IL-6, TNF-?, TC, TG LDL-c ve NEFA seviyeleri model grubundan daha düşük olarak tespit edildi (P

Effect of Exogenous Fibroblast Growth Factor-21 on Inflammation in a High-fat Diet and Cholesterol Mice Model

Fibroblast Growth Factor 21 (FGF21) is a novel metabolic regulator involved in lipid utilization, and it can involve in the regulation of lipid metabolism. To determine the physiological function of FGF21 in relation to a high-fat diet (HFD) and high cholesterol, the effect of FGF21 on immune response indicators and hematological parameters was investigated. In the experiment, a total of 24 female mice were selected, 12 of which were fed with HFDs (group 1) and the other were fed with normal diet (group 2). After 30 days, the mice of two groups were respectively divided into two groups on average: 1. HFDs mice were used as model group; 2 normal diet mice were used as control group; 3. HFDs mice injection were used as model + FGF21 group; 4. normal diet injection were used as control + FGF21 group. Mice were sacrificed at 3-day intervals and the livers were isolated and analyzed. Blood was collected and analyzed for CD3 and CD19 by flow cytometry and IL-4, IL-6 and TNF-? by ELISA. Serum TG, TC, NEFA, LDL-c, and HDL-c levels were measured by automatic biochemical analyzer. Although CD3 and CD19 varied, the difference was not significant, and the levels of IL-4, IL-6, TNF-?, TC, TG LDL-c, and NEFA in the model exogenous injection FGF21 group were lower than in the model group (P<0.05). The present results indicate that exogenous FGF21 regulates IL-4, IL-6 and TNF-? and protects the liver from inflammation damage induced by high dietary fat and cholesterol.

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