? Zongsheng 1 ZHAO, Heng YANG, Yaosheng YU, Yifan XIE, Manjun 1 ZHAI, Huihui LIANG

Koyun RXRG Geninde SNP İdentifikasyonu ve İkizlik İle İlişkisi

RXRG (Retinoik X reseptör-gama) geni ilk olarak hayvan ve insanlarda fetal gelişim ve üreme ile ilişkilendirildi ve büyüme, üreme, metabolizma özellik seleksiyonu ve üreme ile ilgili genetik varyasyonu belirlemek için kullanıldı. Bu çalışmanın amacı, ekzon 2 RXRG gen mutasyonunu ve 313 koyunda ikizlik ile ilişkisini belirleyerek Çin Merinos, Hu ve Kazak koyunlarında yavru sayısı ve genotip frekansını hesaplamaktır. Bu çalışmada RXRG geni exon 1, exon 2 ve exon 10 polimorfizmleri PCR-SSCP ile analiz edildi ve incelenen popülasyon içerisinde P2 parçacığının üç genotipinin anlamlı derecede ikizlik ile ilişkili olduğu belirlendi (P=0.031). Dört grup koyunun analizi sonucunda bir baskın genin (B-allel) bu bireylerde yüksek ikizlik oranına sahip olduğunu ve bu genotipin ikizlik için seçilebileceğini gösterdi. Sonuç olarak, elde ettiğimiz sonuçlar RXRG gen polimorfizminin Marker ilişkili seleksiyon (MAS) yoluyla koyun üretiminde ve genetiğinde kullanılabileceğini önermektedir.

Identification of SNP Within the Sheep RXRG Gene and ItsRelationship with Twinning Trait in Sheep

RXRG (Retinoic X receptor-gamma) gene was originally associated with fetal development and reproduction in human beings and animals, which was used to detect genetic variation that was associated with growth, reproduction, metabolism trait selection and breeding. The aim of this study was to detect RXRG gene mutation of the exon 2 and its association with twinning trait in 313 sheep and calculate litter size, genotype frequency in Chinese Merino, Hu and Kazak sheep. Polymorphism of exon 1, exon 2, exon 10 of RXRG gene in the study were analyzed by PCR-SSCP, which showed that three genotypes of P2 fragment were significantly associated with twinning traits in the analyzed population (P=0.031). Analysis of four groups of sheep showed that there was a predominate gene (B-allele) that have higher twinning rates in these individuals, which indicated that the genotypes can be chosen for twinning according to predominate genotype. In conclusion, our results strongly suggest that polymorphisms of the RXRG gene could be a new choice for sheep breeding and genetics through marker-assisted selection (MAS).

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Merilä J, Sheldon BC: Lifetime reproductive success and heritability in nature. Am Nat, 155, 301-310, 2000. DOI: 10.1086/303330
Wirtanen L, Seguin C: Cloning of cDNAs encoding retinoicacid receptors RAR gamma1, RAR gamma 2, and a new splicing variant, RAR gamma 3, from Aambystomamexicanum and characterization of their expression during early development. Biochim Biophys Acta, 1492, 81- , 2000. DOI: 10.1002/aja.1002010405
Burrage PS, Huntington JT, Sporn MB, Brinckerhoff CE: Regulation of matrixmetallo proteinase gene expression by a retinoid X receptor specific ligand. Arthritis Rheum, 56, 892-2904, 2007. DOI: 10.1093/ bioinformatics/bth199
Clagett DM, Knutson D: Vitamin A in reproduction and development. Nutrients, 3, 385-428, 2011. DOI: 10.3390/nu3040385
Chambon P, Zelent A, Petkovich M, Mendelsohn C, Leroy P, Krust A, Kastner P, Brand N: The family of retinoic acidnuclear receptors. In, Saurat JH (Ed): Retinoids: 10 Years Onstudies. Basel: Karger, 10-27, 1991. DOI: 10.1159/000420323
Michaille JJ, Blanchet S, Kanzler B, Garnier JM: Characterization of cDNAs encoding the chick retinoic acid receptor? and preferential distribution of retinoic acid receptor ? transcripts during chick skin development. Dev Dyn, 201, 334-343, 1994. DOI: 10.1002/aja.1002010405
Zhang XK, Hoffmann B, Tran PBV, Graupner G, Pfahl M: Retinoid X receptor is an auxiliary protein for thyorid hormone and retinoic acid receptor. Nature, 355, 441-446, 1992. DOI: 10.1038/355441a0
Lohnes D, Kastner P, Dierich A, Mark M, LeMeur M, Chambon P: Function of retinoic acid receptor gamma in the mouse. Cell, 73, 643-658, Leid M, Kastner P, Durand B, Krust A, Leroy P, Lyons R: Retinoids acid signal transduction pathways. Ann N YAcad Sci, 684, 19-34, 1993. DOI: 10.1111/j.1749-6632.1993.tb32268.x
Leid M, Kastner P, Chambon P: Mutiplicity generates diversityin the retinoic acid signaling pathways. Trends Biochem Sci, 17, 427-433, DOI: 10.1016/0968-0004(92)90014-Z
Meadus WJ, MacInnis R, Dugan ME. Prolonged dietary treatment with conjugated linoleic acid stimulates porcine muscle peroxisome proliferator activated receptor gamma and glutamine-fructose amino- transferase gene expression in vivo. J Mol Endocrinol, 28, 79-86, 2002. DOI: 10.1677/jme.0.0280079
Nguyen-Huynh NT, Osz J, Peluso-Iltis C, Rochel N, Potier N, Leize- Wagner E: Monitoring of the retinoic acid receptor-retinoid X receptor dimerization upon DNA binding by native mass spectrometry. Biophys Chem, 210, 2-8, 2016. DOI: 10.1016/j.bpc.2015.10.006
Liu QT, John AH: Overlapping PCR for bidirectional PCR amplification of specific alleles: A rapid one-tube method for simultaneously differentiating homozygotes and heterozygotes. Genome Res, 7, 389- , 1997. DOI: 10.1101/gr.7.4.389
Messer L, Wang L, Legault C, Rothschild MF: Mapping and investigation of candidate genes for litter size in French Large White pigs. Animal Genet, 27 (Suppl. 2): 101-119, 1996.
Huang M, Xu SZ, Zan, LS, Zhang, LP, Gao, X, Chen, JB: Genetic variation in RXRG gene and it's relationship with twinning traits in cattle. Yi Chuan=Hereditas, 30, 190-194, 2008. DOI: 10.3724/SP.J.1005. 00190
Wang ZB, Zhang WX, Zhao ZS, Yu P, Wu HB: Establishment of analytical method to evaluate the effect of gene pyramiding in ovine EST- SSR markers about wool traits. J Shihezi Univ (Natural Sci), 2, 8, 2011. DOI: 3969/j.issn.1007-7383.2011.02.009
Sanguinetti CJ. Dias NE. Simpson AJ: Rapid silverstaining and recovery of PCR products separated on polyacrylamide gels. Biotechniques, , 914-921, 1994.
Liu H, Liu C, Yang G, Li H, Dai J, Cong Y, Li X: DNA polymorphism of insulin-like growth factor-binding protein-3 gene and its association with cashmere traits in cashmere goats. Asian-Aust J Anim Sci, 25, 1515, DOI: 10.5713/ajas.2012.12351
Zeng XC, Chen HY, Jia B, Zhao ZS, Hui WQ, Wang ZB, Du YC: Identification of SNPs within the sheep PROP1 gene and their effects on wool traits. Mol Biol Rep, 38, 2723-2728, 2011. DOI: 10.1007/s11033- 0416-4
Guo XH, Chu M, Zhou ZX, Li F, Cheng YS: Study on RARG as a candidate gene for prolificacy of small tailed Han sheep. Chin J Anim Vet Sci, 2006, 37, 756-760.