The ınvestigation of pestivirus and rift valley fever virus ınfections in aborted ruminant foetuses in the blacksea region in turkey
Bu çalışmada, Karadeniz bölgesinin sahil ve iç kesimlerinden elde edilen atık sığır koyun ve keçi fötüsleri pestiviruslar (bovine viral diare virus-BVDV, border hastalığı-BDV) ve rift vadisi humması virus (RVFV) nükleik asitleri yönünden reverz transkriptaz polimeraz zincir reaksiyonu (RT-PZR) ile test edildi. RT-PZR ile 53 atık buzağının 39’unda (%73.6), 37 atık kuzunun 34’ünde (%91.9) ve 2 atık oğlağın tamamında (%100) pestivirus nükleik asidi tespit edildi. Ayrıca aynı atık fötusların tamamında RVFV araştırıldı, fakat hiçbirisinde RVFV nükleik asidi tespit edilemedi.
Karadeniz bölgesinde ruminant atık fötüslerinde pestivirus ve rift vadisi humması virus enfeksiyonlarının araştırılması
In this study, aborted foetuses (cattle, sheep and goat) in either coastal or inland Blacksea region of Turkey were surveyed for the presence of nucleic acids from pestiviruses (bovine viral diarrhoea virus-BVDV, border disease virus-BDV) and rift valley fever virus (RVFV) by reverse transcriptase polymerase chain reaction assay (RT-PCR). Pestivirus nucleic acids were found in 39 of 53 aborted calves (73.6%), in 34 of 37 aborted lambs (91.9%) and in 2 of 2 aborted kids (100%) by RT-PCR. In addition RVFV has been investigated in the same foetuses, however RVFV nucleic acid has not been confirmed in any samples
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- 1. Liu L, Xia H, Wahlberg N, Belak S, Baule C: Phylogeny, classification and evolutionary insights into pestiviruses. Virology, 385, 351-357, 2009.
- 2. Peterhans E, Bachofen C, Stalder H, Schweizer M: Cytopathic bovine viral diarrhea viruses (BVDV): Emerging pestiviruses doomed to extinction. Vet Res, 41, 44, doi: 10.1051/vetres/2010016, 2010.
- 3. Peterhans E, Schweizer M: Pestiviruses: How to outmaneuver your hosts. Vet Microbiol, 142, 18-25, 2010.
- 4. Pepin M, Bouley M, Bird BH, Kemp A, Paweska J: Rift Valley fever virus (Bunyaviridae: Phlebovirus): An update on pathogenesis, molecular epidemiology, vectors, diagnostics and prevention. Vet Res, 41, 61, doi:10.1051/vetres/2010033, 2010.
- 5. Moutailler S, Krida G, Schaffner F, Vazeille M, Failloux AB: Potential vectors of Rift Valley fever virus in the Mediterranean region. Vector Borne Zoonot Dis, 8, 749-754, 2008.
- 6. Hasırcıoğlu S, Kale M, Acar A: Investigation of pestiviruses infections in aborted sheep and goats in Burdur region. Kafkas Univ Vet Fak Derg, 15 (2): 163-167, 2009.
- 7. Vilcek S, Herring AJ, Herring JA, Nettleton PF, Lowings JP, Paton DJ: Pestiviruses isolated from pigs, cattle and sheep can be allocated into at least three genogroups using polymerase chain reaction and restriction endonuclease analysis. Arch Virol, 136, 309-323, 1994.
- 8. Mohamed AE, Imadeldin EA: A simple and rapid method for detection of Rift Valley fever virus in cell culture using RT-PCR. Int J Trop Med, 1, 44-47, 2006.
- 9. Tan MT, Karaoğlu MT, Erol N, Yıldırım Y: Serological and virological investigations of bovine viral diarrhoea virus (BVDV) infection in dairy cattle herds in Aydin province. Turk J Vet Anim Sci, 30, 299-304, 2006.
- 10. Oguzoglu CT, Tan MT, Toplu N, Demir AB, Dagalp SB, Karaoglu T, Ozkul A, Alkan F, Burgu I, Haas L, Greiser-Wilke I: Border disease virus (BDV) infections of small ruminants in Turkey: A new BDV subgroup? Vet Microbiol, 135, 374-379, 2009.
- 11. Oguzoglu CT, Muz D, Yilmaz V, Alkan F, Akca Y, Burgu I: Molecular characterization of Bovine virus diarrhea viruses species 2 (BVDV-2) from cattle in Turkey. Trop Anim Health Prod, 42, 1175-1180, 2010.
- 12. Oguzoglu CT, Muz D, Yilmaz V, Timurkan MO, Alkan F, Akca Y, Burgu I: Molecular characteristics of bovine virus diarrhoea virus 1 isolates from Turkey: Approaches for an eradication programme. Transbound Emerg Dis, doi:10.1111/j.1865-1682-2011.01272.x, 2011.
- 13. Toplu N, Oguzoglu CT, Epikmen ET, Aydogan A: Neuropathologic study of border disease virus in naturally infected fetal and neonatal small ruminants and its association with apoptosis. Vet Pathol, 48, 576-583, 2011
- 14. Yesilbag K, Förster C, Wolf BB, Yilmaz Z, Alkan F, Ozkul A, Burgu I, Rosales SC, Thiel HJ, König M: Genetic heterogeneity of bovine viral diarrhoea virus (BVDV) isolates from Turkey: Identification of a new subgroup in BVDV-1. Vet Microbiol, 130, 258-267, 2008.
- 15. Toplu N, Oguzoglu CT, Albayrak H: Dual infection of fetal and neonatal small ruminants with border disease virus and peste des petits ruminants virus (PPRV): Neuronal tropism of PPRV as a novel finding. J Comp Path, doi:10.1011/j.jcpa.2011.07.004, 2011.
- 16. Azkur AK, Gazyagci S, Aslan ME, Unal N: Molecular and serological characterization of pestivirus infection among sheep in Kirikkale, Turkey. Kafkas Univ Vet Fak Derg, 17 (Suppl-A): S83-S92, 2011.
- 17. Okur-Gumusova S, Yazici Z, Albayrak H: Pestivirus seroprevalence in sheep populations from inland and coastal zones of Turkey. Rev Med Vet-Toulouse, 157, 595-598, 2006.
- 18. Murray RD: A field investigation of causes of abortions in dairy cattle. Vet Rec, 127, 543-547, 1990.
- 19. Carpenter TE, Chrıèl M, Andersen MM, Wulfson L, Jensen AM, Houe H, Greiner M: An epidemiologic study of late-term abortions in dairy cattle in Denmark, July 2000-August 2003. Prev Vet Med, 77, 215-229, 2006.
- 20. Thabti F, Fronzaroli L, Dlissi E, Guibert JM, Hammami S, Pepin M, Russo P: Experimental model of border disease virus infection in lambs: comparative pathogenicity of pestiviruses isolated in France and Tunisia. Vet Res, 33, 35-45, 2002.
- 21. Edmondson MA, Givens MD, Walz PH, Gard JA, Stringfellow DA, Carson RL: Comparison of tests for detection of bovine viral diarrhea virus in diagnostic samples. J Vet Diagn Invest, 19, 376-381, 2007.
- 22. Turkish state meteorological service (TSMS): Update on average annual values of heat, humidity, and rainfall. http//www.dmi.gov.tr/tahmin/il-ve- ilceler.aspx [accessed on 8 October, 2010], 2010.
- 23. Dik B, Yagcı S, Linton YM: A review of species diversity and distribution of Culicoides Latreille, 1809 (Diptera: Ceratopogonidae) in Turkey. J Nat Hist, 40, 1947-1967, 2006.