Ekrem Şanver ÇELİK, Hasan KAYA, SEVDAN YILMAZ

Changes in hematological and biochemical parameters of european chub (Squalius cephalus L.) in unpolluted reservoir and polluted creek

Bu çalışmada, Turkiye’nin batısında yeralan Marmara bölgesi’ndeki Atikhisar barajı ve Sarıçay’ın yüzey suyu kalite kriterleri 2006 Ağustos ayı boyunca incelenmiştir. Sarıçay’ın iletkenlik, COD, BOD, TP, TN ve TSS içeriği Atikhisar barajından yüksek bulunmuş (P

Avrupa tatlısu kefali (Squalius cephalus L.)’nin temiz baraj ve kirli çay’daki hematolojik ve biyokimyasal parametrelerindeki değişimler

This study investigates the surface water quality parameters of the Atikhisar Reservoir and Sarıçay Creek, both of which are located in Çanakkale, the north-west part of Turkey. The conductivity, COD, BOD, TP, TN, and TSS content of water in Sarıçay Creek were higher than in Atikhisar Reservoir (P<0.05), however; water DO content in Sarıçay Creek was less than in Atikhisar Reservoir (P<0.05). Chl-a concentration, temperature, salinity and pH of water were not statistically significant in both of the resources (P>0.05). The quality of surface water samples of Atikhisar Reservoir and Sarıçay Creek were accepted first quality and third quality, respectively. The blood samples of European chub (Squalius cephalus L.) caught in the Atikhisar Reservoir (unpolluted) and Sarıçay Creek (polluted) provided the data to compare the hemato-chemical effects of pollution. The WBC, MCV, MCH, Ht, Hb, GLC, TP, and GLO levels of fish in Sarıçay Creek were significantly higher than those in Atikhisar Reservoir (P<0.05). The RBC, TBIL, CHOL, AST, and ALT levels of fish in Sarıçay were lower than the ones in Atikhisar Reservoir (P<0.05). There were no statistically significant differences in the MCHC, TG, ALB, Ca, and P values among the fish samples of Atikhisar Reservoir and Sarıçay (P>0.05). In conclusion, the data analyses infer that blood parameters can be used as an indicator of the fish health in general. On the other hand, the blood test can be used to determine fish stress due to water pollution.

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1. Martins CIM, Ochola D, Ende SSW, Eding EH, Verreth JAJ: Is growth retardation present in Nile tilapia Oreochromis niloticus cultured in low water exchange recirculating aquaculture systems? Aquaculture, 298 (1-4): 43-50, 2009.
2. Akbulut M, Kaya H, Celik ES, Odabasi DA, Odabasi SS, Selvi K: Assessment of surface water quality in the Atikhisar Reservoir and Sarıçay Creek (Canakkale, Turkey). Ecology, 19 (74): 139-149, 2010.
3. APHA, AWWA, WEF: Standard Methods for the Examination of Water and Wastewater. 18th ed. APHA, AWWA, WPCF, Washington DC, 1992.
4. Bullock AM: Laboratory methods. In, Roberts RJ (Ed): Fish Pathology. 1st ed., pp. 374-405, Baillière & Tindall, London, 1989.
5. Busacker GP, Adelman IR, Goolish EM: Growth. In, Schreck CB Moyle PB (Eds): Methods for Fish Biology. 1st ed., pp. 363-382, American Fisheries Society, Bethesda, 1990.
6. Statistically Analytical System: Statistical graphics system: By Statistical Graphics Corporation, User’s Guide-Reference. STCS, Inc, 1996.
7. Schendel EK, Nordström SE, Lavkulich LM: Floc and sediment properties and their environmental distribution from a marine fish farm. Aquac Res, 35 (5): 483-493, 2004.
8. Azim ME, Little DC: The biofloc technology (BFT) in indoor tanks: water quality, biofloc composition, and growth and welfare of Nile tilapia (Oreochomis niloticus). Aquaculture, 283 (1-4): 29-35, 2008.
9. Vanderploeg HA, Ludsin SA, Cavaletto JF, Höök TO, Pothoven SA, Brandt SB, Liebig JR, Lang GA: Hypoxic zonesas habitat for zooplankton in Lake Erie: refuges from predation or exclusion zones. J Exp Mar Biol Ecol, 381 (1): 108-120, 2009.
10. Padmini E, Rani MU: Evaluation of oxidative stress biomarkers in hepatocytes of grey mullet inhabiting natural and polluted estuaries. Sci Total Environ, 407 (15): 4533-4541, 2009.
11. WHO: Guidelines for Drinking-Water Quality. WHO, Geneva, Switzerland, 2004.
12. WPCR: Water Pollution Control Regulation. 31.12.2004 dated official newspaper, Ankara, Turkey, 2004.
13. Noga EJ, Wang C, Grindem CB: Comparative clinicophathological responses of stripped bass and palmetto bass to acute stress. T Am Fish Soc, 128 (4): 680-686, 1999.
14. Adham KG: Sublethal effects of aquatic pollution in Lake Maryût on African sharptooth catfish, Clarias gariepinus (Burchell, 1822). J Appl Ichthyol, 18 (2): 87-94, 2002.
15. Adham KG, İbrahim HM, Hamed SS, Saleh RA: Blood chemistry of the Nile tilapia, Oreochromis niloticus (Linnaeus, 1757). Aquat Ecol, 36 (4): 549-557, 2002.
16. Aride PHR, Roubach R, Val AL: Tolerance response of tambaqui Colossoma macropomum (Cuvier) to water pH. Aquac Res, 38 (6): 588-594, 2007.
17. Zhou X, Li M, Abbas K, Wang W: Comparison of haematology and serum biochemistry of cultured and wild Dojo loach Misgurnus anguillicaudatus. Fish Physiol Biochem, 35 (3): 435-441, 2009.
18. John PJ: Alteration of certain blood parameters of freshwater teleost Mystus vittatus after chronic exposure to Metasystox and Sevin. Fish Physiol Biochem, 33 (1): 15-20, 2007.
19. Yang JL, Chen HC: Effects of gallium on common carp (Cyprinus carpio): Acute test, serum biochemistry, and erythrocyte morphology. Chemosphere, 53 (8): 877-882, 2003.
20. Jee JH, Park KH, Keum YH, Kang JC: Effects of 7, 12-dimethylbenz(a)anthracene on growth and haematological parameters in Korean rockfish, Sebastes schlegeli (Hilgendorf). Aquac Res, 37 (5): 431-442, 2006.
21. Gopal V, Parvathy S, Balasubramanian RS: Effect of heavy metals on the blood protein biochemistry of the fish Cyprinus carpio and its use as a bio-indicator of pollution stress. Environ Monit Assess, 48 (2): 117-124, 1997.
22. Coz-Rakovac R, Strunjak-Perovic I, Hacmanjek M, Topic PN, Lipej Z, Sostaric B: Blood chemistry and histopathological properties of wild and cultured sea bass (Dicentrarchus labrax) in the north Adriatic Sea. Vet Res Commun, 29 (8): 677-687, 2005.
23. Cornelius CE: Bile pigments in fishes: A review. Vet Clin Path, 20 (4): 106-114, 1992.
24. Pepelinjak S, Petrinec Z, Kovacic S, Segvic M: Screening toxicity study in young carp (Cyprinus carpio L.) on feed amended with fumonisin B1. Mycopathologia, 156 (2): 139-145, 2002.
25. Bernet D, Schmidt H, Wahli T, Burkhardi-Holm P: Effluent from a sewage treatment works causes changes in serum chemistry of brown trout (Salmo trutta L.). Ecotox Environ Safe, 48 (2): 140-147, 2001.
26. Chen Y, Wooster GA, Getchell RG, Bowser PR, Timmons MB: Blood chemistry of healthy, nephrocalcinosis-affected and ozone-treated tilapia in a recirculation system, with application of discriminant analysis. Aquaculture, 218 (1-4): 89-102, 2002.
27. Shah SL: Haematological parameters in tench, Tinca tinca after short term exposure to lead. J Appl Toxicol, 26 (3): 223-228, 2006.
28. Qureshi IZ, Hafeez MA, Shaikh SA: Hematological response of the fish, Cyprinus watsoni, to hypoxia, anemia and hyperthermia. Pak J Zool, 27, 219-227, 1995.
29. Val A, Lessard J, Randall DJ: Effects of hypoxia on rainbow trout (Oncorhynchus mykiss) - intraerythrocytic phosphates. J Exp Biol, 198 (2): 305-310, 1995.