NAZAN GEZER İNCE, GÜLSÜN PAZVANT, ÇAĞDAŞ OTO, OYA KAHVECİOĞLU

Stereological measurement of testicular volume in kivircik rams

Türkiye’nin ulusal bir koyun ırkı olan Kıvırcık koçlarında testis hacmini stereolojik bir yöntemle hesaplama amaçlandı. Karkas ağırlıkları alınmış 15 adet 5 aylık, 14 adet 6 aylık, 13 adet 7 aylık olmak üzere 42 adet Kıvırcık koçtan sağ ve sol testisler epididymis ile birlikte alındı. Testislerin ağırlık ve morfometrik ölçümleri (uzunluk, genişlik, kalınlık) alındı. Testis ve epididymis’lerin Arşimet prensibine göre hacimleri hesaplandı. Cavalieri prensibine göre 0.6 cm kalınlığında olmak üzere 8 ile 11 adet paralel kesitler alındı. Bu kesitlerde tüm testis (testis+epididymis) hacmi için 0.8; testis ve epididymis hacmi için 0.4 cm sonda aralıklı gridler kesitler üzerine rastgele atılarak üzerinde çakışan noktalar sayıldı. Hacim, hacim oranı ve hata katsayısı hesaplama formüllerini içeren Microsoft Excel (XP sürümü) programı kullanılarak değerler otomatik olarak hesaplandı. Çalışmada Arşimet ve Cavalieri metotları kullanılarak elde edilen hacim değerleri ile sağ ve sol testis hacim değerleri arasında istatistiksel fark gözlenmedi (P>0.05). Cavalieri metodu kullanılarak aylara göre ayrı ayrı hesaplanan epididymis, testis ve tüm testis hacimlerindeki en hızlı artış 6 aylık ile 7 aylık koçlar arasında gözlendi. Ortalama değerler kullanılarak aylara göre yapılan korelasyon analizinde 6 aylık koçlarda karkas ağırlığının epididymis hacmi ile korelasyon gösterdiği saptandı (P

Anahtar Kelimeler:

kestirim, Kıvırcık, vücut ölçümleri, vücut ağırlığı, testis, koyun, regresyon analizleri, koç, yerli çiftlik hayvanları, morfometri, metodoloji, denklemler, epididimis, korelasyon analizleri, karkas ağırlığı

Kıvırcık koçlarında testis hacminin stereolojik ölçümleri

In this study, measurement of testis volume by stereology in Kivircik rams, Turkey’s traditional sheep breed, was purposed. Right and left testes with epididymis were taken from a total of 42 Kivircik rams whose carcass weights were measured previously. Fifteen, 14, and 13 of them were 5, 6, and 7 months old, respectively. Weights and morphometric measurements (length, width, and thickness) of the testis and epididymis were taken. Theirs volumes were estimated according to the Archimedes principle. According to Cavalier’s principle, 0.6 cm thick 8-11 parallel sections were taken. In these sections for whole testis (testis + epididymis) volume 0.8 cm, and for testis and epididymis volumes, grids with 0.4 cm intervals randomly placed on sections and convergent points were counted. By using Microsoft Excel (XP version), contained volume, volume ratio and coefficient error estimation formulas were used for estimating values automatically. In the study, statistical difference was not observed (P>0.05) between right and left testes volumes values acquired from Archimedes and Cavalier’s methods. The highest increase in epididymis, testis, and whole testis volumes, which were measured separately, was observed between rams at 6-7 months of age. In correlation analyses, estimated according to months by using mean values, it was recorded that there was a correlation between carcass weight and epididymis volume (P<0.05). Correlation was observed by ratio of P<0.01 between epididymis volume and testis thickness for 7 months old rams. Although there was no correlation observed between testis volume and epididymis volume for 7 months old rams. Instead of above evaluations, correlation ratio of P<0.01 was observed between morphometric measurements and stereological data of all analysis. Regarding features, showed higher correlations, regression analyses were made. In the presence of some morphometric data, regression equations, which required us to get stereological data, were calculated. Our thought is the collected data of this study can support the further experimental animal studies.

Keywords:

estimation, Kivircik, body measurements, body weight, testes, sheep, regression analysis, rams, native livestock, morphometrics, methodology, equations, epididymis, correlation analysis, carcass weight,

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