SERHAT KESER, ORHAN ERMAN, ÖKKEŞ YILMAZ, SEVGİ İRTEGÜN KANDEMİR, Mehmet TUZCU

Effects of antioxidants resveratrol, catechin and lipoic acid and carcinogen kbro3 on lipophylic vitamins and cholesterol in lung, liver and kidney of wistar rats

Bu çalışmanın amacı kanserojen KBrO3’e karşı resveratrol, lipoik asit ve kateşin gibi antioksidanların Wistar ratların karaciğer, akciğer ve böbrek dokularında lipofilik vitaminler ve kolesterol üzerindeki etkilerini incelemektir. Bu çalışmada, toplam 50 Wistar sıçan rasgele beş gruba dağıtıldı: 1. Kontrol (C), 2. KBrO3 (K), (40 mg/kg dozunda haftada iki kez), 3. Kateşin (30 mg/kg dozunda haftada dört kez) + KBrO3 (40 mg/kg dozunda haftada iki kez) (Cat), 4. Lipoik Asit (30 mg/kg dozunda haftada dört kez) + KBrO3 (40 mg/kg dozunda haftada iki kez) (LA) and 5. Resveratrol (30 mg/kg dozunda haftada dört kez) + KBrO3 (40 mg/kg haftada iki kez) (R). Bütün uygulamalar 5 hafta boyunca sürdürüldü ve sonra her bir deney sıçanı dekapite edildi ve dokuları alınarak biyokimyasal analizlere kadar -85°C’de saklandı. Akciğer, karaciğer ve böbrek dokularında lipofilik vitaminler ve kolesterol seviyeleri HPLC cihazıyla belirlendi. Sonuçlarımıza göre, akciğer dokusunda kolesterol seviyesi K, Cat, LA ve R gruplarında, α-tokoferol seviyesi ise K ve R gruplarında azalmıştır. Karaciğer dokusunda, α-tokoferol, retinol ve kolesterol seviyeleri K, Cat, LA ve R gruplarında artmıştır. Böbrek dokusunda, δ-tokoferol, vitamin D2, vitamin D3 ve α-tokoferol seviyeleri K, Cat, LA ve R gruplarında azalırken, kolesterol seviyesi Cat, LA ve R gruplarında artmıştır. Sonuç olarak, sonuçlarımız göstermiştir ki, resveratrol, lipoik asit, kateşin ve potasyum bromat uygulaması Wistar sıçanlarda lipofilik vitaminler ve kolesterol seviyelerini etkilemiştir ve bu uygulamalar sıçanlarda kolesterol biyosentezini etkilemiş olabilir.

Anahtar Kelimeler:

sıçan, alfa-tokoferol, antioksidanlar, biyosentez, karsinojenler, kateşin, kolekalsiferol, kolesterol, ergokalsiferol, böbrekler, karaciğer, akciğerler, retinol, tiyoaktik asit, vitaminler

Wistar sıçanların akciğer, karaciğer ve böbrek dokularında lipofilik vitaminler ve kolesterol üzerinde antioksidanlar resveratrol, kateşin ve lipoik asit ile kanserojen kbro3’ın etkileri

The aim of this research is to examine effects of antioxidants resveratrol, lipoic acid, catechin and carcinogen potassium bromate on the level of lipophylic vitamins and cholesterol in liver, lung and kidney tissue of Wistar albino rats. In this study, total 50 Wistar rats were randomly divided into five groups: 1. Control (C), 2. KBrO3 (K) (40 mg/kg two times per week), 3. Catechin (30 mg/kg four times per week) + KBrO3 (40 mg/kg two times per week) (Cat), 4. Lipoic Acid (30 mg/kg four times per week) + KBrO3 (40 mg/kg two times per week) (LA) and 5. Resveratrol (30 mg/kg four times per week) + KBrO3 (40 mg/kg two times per week) (R). All treatments were continued for 5 weeks, after which time each experimental rat was decapitated and tissues were collected and stored in -85°C prior to biochemical analysis. In lung, liver and kidney, lipophylic vitamins and cholesterol were measured by HPLC. According to our results, while the cholesterol level was decreased in the K, Cat, LA and R groups, the α-tocopherol level was decreased in K and R groups of the lung tissue. α-tocopherol, cholesterol and retinol levels were increased in K, Cat, LA and R groups of the liver tissue. While the δ-tocopherol, vitamin D2, vitamin D3 and α-tocopherol levels were decreased in K, Cat, LA and R groups, the cholesterol level was increased in Cat, LA and R groups of kidney tissue. In conclusion, our results indicated that the applications of resveratrol, lipoic acid, catechin and potassium bromate influenced cholesterol and lipophylic vitamins levels and these applications can be affected cholesterol biosynthesis in Wistar albino rats.

Keywords:

rats, alpha-tocopherol, antioxidants, biosynthesis, carcinogens, catechin, cholecalciferol, cholesterol, ergocalciferol, kidneys, liver, lungs, retinol, thioctic acid, vitamins,

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