Asghar GHAHREMANI, Ali Asghar SADEGHI, Saeed HESARAKI, Mohammad CHAMANI, Parvin SHAWRANG

Effect of Energy Sources and Levels on Caecal Microbial Population, Jejunal Morphology, Gene Expression of Jejunal Transporters (SGLT1, FABP) and Performance of Broilers Under Heat Stress

Bu çalışma; enerji kaynakları ve seviyelerinin ısı stresi altında mikrobiyal popülasyona, jejunum morfolojisine, glukoz transporteri (SGLT1) ve yağ asitleri bağlayıcı protein (FABP) gen ekspresyonlarına ve broiler performansına etkilerini araştırmak amacıyla yürütülmüştür. Rastgele örneklemeyle, 600 adet 1 günlük Cobb broiler civciv, 4 tekrar olmak üzere 5 farklı beslenme uygulanmasına alındı. Civcivler ana enerji kaynağı olarak mısıra dayalı diyetle beslendi. Kontrol grubuna (C) bazal seviyede Cobb standardına göre yem verilirken, T1 grubuna kontrole göre %3 daha düşük enerjili yem, T2 grubuna kontrole göre %6 daha düşük enerjili yem, T3 grubuna Cobb standardına göre mısır ve soya fasulyesi yağı tabanlı diyet ve T4 grubuna kontrole göre %3 daha fazla enerjili mısır ve soya fasulyesi yağı tabanlı diyet uygulandı. Isı stresi oluşturmak amacıyla 21. günden 41. güne kadar günde 8 saat boyunca sıcaklık 34°C'ye çıkarıldı. T3 ve T4 grubundaki civcivlerde kontrol grubuna oranla Lactobacillus popülasyonu daha yüksek iken Escherichia coli popülasyonu daha düşüktü (P

Isı Stresi Altında Enerji Kaynakları ve Seviyelerinin Sekum Mikrobiyal Popülasyonuna, Jejunum Morfolojisine, Jejunal Transporterlerin (SGLT1, FABP) Gen Ekspresyonuna ve Broiler Performansına Etkileri

The present study was conducted to evaluate the effects of energy sources and levels on microbial population, jejunal morphology, gene expression of glucose transporter (SGLT1), fatty acid binding protein (FABP) and performance in broilers under heat stress. In a completely randomized design, 600 one-day-old Cobb broiler chickens were assigned to five dietary treatments and four replicates. Chicks were fed diet based on corn as main energy source and energy level based on Cobb standard considered as control (C), corn based diet with 3% lesser energy than control (T1), corn based diet with 6% lesser energy than control (T2), corn and soybean oil based diet according to Cobb standard (T3), corn and soybean oil based diet with 3% upper energy than control (T4). Temperature was increased to 34°C for 8 hours daily from day 21 to 41 to induce heat stress. Chickens in T3 and T4 had higher Lactobacillus population and lower Escherichia coli population than C group (P<0.05). Chickens in T1 and T2 had shorter jejunal villi, deeper crypts, and lower villus height: crypt depth than those fed C, T3 and T4 diets (P<0.05). There were no significant differences among treatments for gene expression of both nutrient transporters (P>0.05). Chickens in T3 and T4 had higher weight gain compared to C, T1 and T2 (P<0.05). Feed intake in T3 was lower than C, consequently, feed conversion ratio of chicks fed T3 was better than C group (P<0.05). In conclusion, replacement a part of dietary energy source with soybean oil might improve intestinal parameters and performance of broilers under heat stress

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Windhorst HW: Changes in poultry production and trade worldwide. World's Poult Sci J, 62, 585-602, 2006. DOI: 10.1079/WPS2006114
Lin H, Jaio HC, Buyse J, Decuypere E: Strategies for preventing heat stress in poultry. World's Poult Sci J, 62, 71-85, 2006. DOI: 10.1079/ WPS200585
Schlesinger MJ, Ryan C, Chi MM, Carter JG, Pusateri ME, Lowry OH: Metabolite changes associated with heat shocked avian fibroblast mitochondria. Cell Stress Chaperones, 2, 25-30, 1997.
Lan PT, Sakamoto M, Benno Y: Effects of two probiotic Lactobacillus strains on jejunal and cecal microbiota of broiler chicken under acute heat stress condition as revealed by molecular analysis of 16S rRNA genes. Microbiol Immunol, 48, 917-929, 2004. DOI: 10.1111/j.1348-0421.2004. tb03620.x
Tao X, Zhang ZY, Dong H, Zhang H, Xin H: Responses of thyroid hormones of market-size broilers to thermoneutral constant and warm cyclic temperatures. Poult Sci, 85, 1520-1528, 2006. DOI: 10.1093/ ps/85.9.1520
Sohail MU, Hume ME, Byrd JA, Nisbet DJ, Ijaz A, Sohail A, Shabbir MZ, Rehman H: Effect of supplementation of prebiotic mannan-oligo- saccharides and probiotic mixture on growth performance of broilers subjected to chronic heat stress. Poult Sci, 91, 2235-2240, 2012. DOI: 3382/ps.2012-02182
Yu J, Yin P, Liu F, Cheng G, Guo K, Lu A, Zhu X, Luan W, Xu J: Effect of heat stress on the porcine small intestine: a morphological and gene expression study. Comp Biochem Physiol, 156, 119-28, 2010. DOI: 1016/j.cbpa.2010.01.008
Quinteiro-Filho WM, Ribeiro A, Ferraz-de-Paula V, Pinheiro ML, Sakai M, Sa LRM, Ferreira AJP, Palermo-Neto J: Heat stress impairs performance parameters, induces intestinal injury, and decreases macrophage activity in broiler chickens. Poult Sci, 89, 1905-1914, 2010. DOI: 10.3382/ps.2010-00812
Menten JFM, Barbosa Filho JAD, Silva MAN, Silva IJO, Racanicci AMC, Coelho AAD, Savino VJM: Physiological responses of broiler chickens to pre-slaughter heat stress. World´s Poult Sci J, 62, 254-257, 2006.
Aksit M, Yalcin S, Ozkan S, Metin K, Ozdemir D: Effects of temperature during rearing and crating on stress parameters and meat quality of broilers. Poult Sci, 85, 1867-1874, 2006. DOI: 10.1093/ps/85.11.1867
Kohl KD: Diversity and function of the avian gut microbiota. J Comp Physiol B, 182,591-602, 2012. DOI: 10.1007/s00360-012-0645-z
Apajalahti JHA, Kettunen A, Bedford MR, Holben WE: Percent G+C profiling accurately reveals diet-related differences in the gastrointestinal microbial community of broiler chickens. Appl Environ Microbiol, 67, 5656- , 2001.DOI: 10.1128/AEM.67.12.5656-5667.2001
Soltan M: Influence of dietary glutamine supplementation on growth performance, small intestinal morphology, immune response and some blood parameters of broiler chickens. Int J Poult Sci, 8, 60-68, 2009. DOI: 3923/ijps.2009.60.68
Mitchell MA, Carlisle AJ: The effect of chronic exposure to elevated environmental temperature on intestinal morphology and nutrient absorption in the domestic fowl (Gallus domesticus). Comp Biochem Physiol, 101, 137-142, 1992. DOI: 10.1016/0300-9629(92)90641-3
Thorens B: Glucose transporters in the regulation of intestinal, renal and liver glucose fluxes. Am J Physiol, 270, 541-553, 1996.
Wright EM, Turk E: The sodium/glucose co-transporter family SLC5. Eur J Physiol, 447, 510-518, 2004. DOI: 10.1007/s00424-003-1063-6
Furuhashi M, Hotamisligil GS: Fatty acid-binding proteins: role in metabolic diseases and potential as drug targets. Nat Rev Drug Discov, , 489-503, 2008. DOI: 10.1038/nrd2589
Gal-Garber OS, Mabjeesh J, Sklan D, Uni Z: Partial sequence and expression of the gene for and activity of the sodium glucose transporter in the small intestine of fed, starved and refed chickens. J Nutr, 130, 2174- , 2000.
Naruhashi K, Sai Y, Tamai I, Suzuki N, Tsuji A: PepT1 mRNA expression is induced by starvation and its level correlates with absorptive transport of cefadroxial longitudinally in the rat intestine. Pharm Res, 19, 1423, 2002. DOI: 10.1023/A:1020436028194
Wang X, Peebles ED, Zhai W: Effects of protein source and nutrient density in the diets of male broilers from 8 to 21 days of age on their subsequent growth, blood constituents, and carcass compositions. Poult Sci, 93, 1463-1474,2014. DOI: 10.3382/ps.2013-03838
Zai W, Peebles ED, Zumwalt CD, Mejia L, Corozo A: Effects of dietary amino acid density regimens on growth performance and meat yield of Cobb×Cobb 700 broilers. J Appl Poult Res, 22, 447-460, 2013. DOI: 3382/japr.2012-00658
Daghir NJ: Broiler feeding and management in hot climates. In, Daghir NJ (Ed): Poultry Production in Hot Climate. 185-218, CAB Intl, Wallingford, UK, 1995.
Zulkifli I, Liew PK, Israf DA, Omar AR, Hair-Bejo M: Effects of early age feed restriction and thermal conditioning on heterophil/lymphocyte ratio, heat shock protein 70 and body temperature of male broiler chickens subjected to acute heat stress. J Thermal Biol, 28, 217-222, 2003. DOI: 10.1016/s0306-4565(02)00058-x
Uni Z, Noy Y, Sklan D: Post-hatch development of smallintestinal function in the poultry. Poult Sci, 78, 215-222, 1999. DOI: 10.1093/ps/78.2.215
Livak KJ, Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2-??CTmethod. Methods, 25, 402-408, DOI: 10.1006/meth.2001.1262
Xu ZR, Hu CH, Xia MS, Xia X, Zhan A, Wang MQ: Effects of dietary fructo-oligosaccharide on digestive enzyme activities, intestinal microflora and morphology of male broilers. Poult Sci, 82, 1030-1036, DOI: 10.1093/ps/82.6.1030
Wang X, Peebles ED, Morgan TW, Harkess RL, Zhai W: Protein source and nutrient density in the diets of male broilers from 8 to 21 d of age: Effects on small intestine morphology. Poult Sci, 94, 61-67, 2015. DOI: 3382/ps/peu019
SAS Institute Inc: SAS/STAT 9.13 User's Guide. SAS Inst Inc, Cary, NC, 2004.
Geyra A, Uni Z, Sklan D: Enterocyte dynamics and mucosal development in the post-hatch chick. Poult Sci, 80, 776-782, 2001. DOI: 1093/ps/80.6.776
Sklan D, Geyra A, Tako E, Gal-Gerbert O, Uni Z: Ontogeny of brush border carbohydrate digestion and uptake in the chick. Br J Nutr, 89, 747- , 2003. DOI: 10.1079/BJN2003853
Yaqoob P: Fatty acids and the immune system: from basic science to clinical applications. Proceed Nutr Soc, 63, 89-104, 2004. DOI: 10.1079/ PNS2003328
Sadeghi A, Mirmohseni A, Shawrang P, Aminafshar M: The effect of soy oil addition to the diet of broiler chicks on the immune response. Turk J Vet Anim Sci, 37, 264-270, 2013. DOI: 10.3906/vet-1109-24
Cherian G: Nutrition and metabolism in poultry: Role of lipids in early diet. J Anim Sci Biotechnol, 6, 28-35, 2015. DOI: 10.1186/s40104-015- 9
Geraert PA, Padilha JCF, Guillaumin S: Metabolic and endocrine changes induced by chronic heat exposure in broiler chickens: Biological and endocrinologicalvariables. Br J Nutr,75, 205-216, 1996. DOI: 10.1017/ BJN19960125
Lei KY, Slinger SJ: Energy utilization in the chick in relation to certain environmental stresses. Can J Anim Sci, 50, 285-292, 1970. DOI: 10.4141/ cjas70-043
Yamauchi K, Yamamoto K, Ishiki Y: Morphological alterations of the intestinal villi and absorptive epithelial cells in each intestinal part in fasted chickens. Jpn J Poult Sci, 32, 241-251, 1995. DOI: 10.2141/ jpsa.32.241
Hu XF, Guo YM: Corticosterone administration alters small intestinal morphology and function of broiler chickens. Asian-Australas J Anim Sci, , 1773-1778, 2008. DOI: 10.5713/ajas.2008.80167
Gartner LP, Hiatt JL: Color Textbook of Histology. 2nd edn., WB Saunders, Baltimore, MD, USA, 2001.
Griffiths L, Leeson S, Summers JD: Influence of energy system and level of various fat sources on performance and carcass composition of broiler. Poult Sci, 56, 1018-1026, 1977. DOI: 10.3382/ps.0561018
Nitsan Z, Dvorin A, Zoref Z, Mokady S: Effect of added soybean oil and dietary energy on metabolisable and net energy of broiler diets. Br Poult Sci, 38, 101-106,1997. DOI: 10.1080/00071669708417948
Markovic R, Sefer D, Krstic M, Petrujkic B: Effect of different growth promoters on broiler performance and gut morphology. Archiv Med Vet, (2): 442-448, 2009.
Freitas KC, Gabriel JE, Leite LC, de Armas RD, Lanna DPD Madeira HMF: Molecular characterization of ruminal bacterial diversity. Acta Scientific Anim Sci, 30, 187-192, 2008. DOI: 10.4025/actascianimsci. v30i2.4699
Choct M, Hughes RJ, Wang J, Bedford M, Morgan AJ, Annison G: Increased small intestinal fermentation is partly responsible for the anti- nutritive activity of non-starch polysaccharides in chickens. Br Poult Sci, , 609-621, 1996. DOI: 10.1080/00071669608417891
Innis SM, Pinsk V, Jacobson K: Dietary lipids and intestinal inflammatory diseases. J Peds, 58, 89-95, 2006. DOI: 10.1016/j.jpeds.2006.06.058
Laflamme DR, Xu H, Long GM: Effect of diets differing in fat content on chronic diarrhea in cats. J Vet Intern Med, 25, 230-235, 2011. DOI: 1111/j.1939-1676.2010.0665.x
Adibi S: Regulation of expression of the intestinal oligopeptide transporter (PepT-1) in health and disease. Am J Physiol-Gastrointestinal Liver Physiol, 285, 779-788, 2003. DOI: 10.1152/ajpgi.00056.2003
Sun X, Zhang H, Sheikhahmadi A, Wang Y, Jiao H, Lin H, Song Z: Effects of heat stress on the gene expression of nutrient transporters in the jejunum of broiler chickens (Gallus gallus domesticus). Intl J Biometeorol, 59, 127-135, 2015. DOI: 10.1007/S00484-014-0829-1
Shepherd EJ, Helliwell PA, Mace OJ, Morgan EL, Patel N, Kellett GL: Stress and glucocorticoid inhibit apical Glut2-trafficking and intestinal glucose absorption in rat small intestine. J Physiol, 560, 281-290, 2004. DOI: 10.1113/jphysiol.2004.072447
Hu XF, Guo YM, Huang BY, Bun S, Zhang LB, Li JH, Liu D, Long FY, Yang X, Jiao P: The effect of glucagon-like peptide 2 injection on performance, small intestinal morphology, and nutrient transporter expression of stressed broiler chickens. Poult Sci, 89, 1967-1974, 2010. DOI: 10.3382/ps.2009-00547
Hurwitz S, Weiselberg M, Eisner M, Bartov I, Riesenfeld G, Sharvit M, Niv A, Bornstein S: The energy requirements and performance of growing chickens and turkeys as affected by environmental temperature. Poult Sci, 59, 2209-2299, 1980. DOI: 10.3382/ps.0592290
Min YN, Shi JS, Wei FX, Wang HY, Hou XF, Niu ZY, Liu FZ: Effects of dietary energy and protein on growth performance and carcass quality of broilers during finishing phase .J Anim Vet Advances, 11, 3652-3657, 2012. DOI: 10.3923/javaa.2012.3652.3657
Dairo F, Adesehinwa ASAOK, Oluwasola TA, Oluyemi JA: High and low dietary energy and protein levels for broiler chickens. Afr J Agri Res, (15): 2030-2038, 2010.
Das GB, Hossain ME, Akbar MA: Effects of different oils on productive performance of broiler. Iranian J Appl Anim Sci, 4 (1): 111-116, 2014.
Monfaredi A, Rezaei M, Sayyahzadeh H: Effect of supplemental fat in low energy diets on some blood parameters and carcass characteristics of broiler chicks. S Afr J Anim Sci, 41, 24-32, 2011.