F. Eser ÖZGENCİL, Nurhayat GÜLMEZ, Çağrı GÜLTEKİN, Serkan SAYINER

Corneal Impression Cytology for the Diagnosis of Limbal Stem Cell Deficiency in a Dog

Yakın Doğu Hayvan Hastanesine kırmızı göz şikayeti ile getirilen 14 yaşlı kısırlaştırılmış, dişi, Terrier ırkı bir köpekte oftalmoskopik muayene sonucu sağ gözde, saat 10-02 pozisyonunda korneanın proksimal 1/3'ünü kaplayan konjunktivadan köken alan korneal yüzeyel vaskülarizasyon, fotofobi ve görmede azalma saptandı. Schirmer göz yaşı testi normal ve florescein boyama negatif olan gözde, damarların regresyonu için uygulanan topical kortikosteroit, antibiotik ve sikloplejik kombinasyonundan olumlu bir sonuç alınamadı ve semptomların artması ve gerilememesi üzerine olgunun kan ve serum örnekleri, insanlardaki olası limbal kök hücre yetmezliği (LKHY) etiyololojik faktörlerini irdelemek ve bölgesel yaygın kan hastalıkları ile ilişkilendirmek amacı ile hematolojik, biyokimyasal ve serolojik analizler için toplandı. Test sonuçları normal sınırlarda olduğu gözlenen olguda korneal impresyon sitolojisi gerçekleştirildi. LKHY tanısında altın standart olan korneada konjunktivalizasyonun göstergesi goblet hücrelerinin gözlenmesi ile veteriner hekimlikte ilk kez oküler yüzey bozukluğu olan bir olguda idiopatik parsiyel LKHY tanısı kondu

Bir Köpekte Limbal Kök Hücre Yetmezliğinin Tanısında Korneal İmpresyon Sitolojisi: Olgu Sunumu

A 14-year old, castrated, female terrier was referred to Near East Animal Hospital with a complaint of red eye. Ophthalmoscopic examination revealed superficial corneal vascularization originating from conjunctiva and encompassing proximal one-third of cornea in 10-02 o'clock position together with photophobia and vision loss. Schirmer tear test was normal and fluorescein staining was negative for the affected eye. Since combined treatment with topical corticosteroid, antibiotic and cycloplegic did not improve the vascular regression, rather exacerbated the symptoms, blood and serum samples were collected for hematologic, biochemical and serologic analyses in order to investigate possible etiologic factors in human LSCD and to define its association with prevalent local blood disorders. As hematologic, biochemical and serologic test results were within the normal ranges, corneal impression cytology was conducted. Upon the observation of goblet cells the gold standard for the diagnosis of LSCD and the indicative of conjunctivalization in cornea the diagnosed of idiopathic partial limbal stem cell deficiency was confirmed in the dog in which it was the first case with ocular surface defect in veterinary medicine

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