Ayfer YILDIZ, Emin KARAKURT, Serpil DAĞ, Hilmi NUHOĞLU, Enver BEYTUT, Emre KURTBAŞ

Pulmoner Adenokarsinomlu Koyunlarda Metastaz ve MMP-9 İlişkisi

Bu çalışmada, Matriks Metalloproteinaz-9’un koyunların pulmoner adenokarsinom olgularının metastazındaki rolünün immünohistokimyasal yöntemlerle değerlendirilmesi amaçlandı. Rutin histopatolojik tanı için bölümümüze getirilen 26 koyundan akciğer dokusu örnekleri alındı. Akciğer dokuları %10’luk tamponlu formalinde tespit edildi, rutin prosedürler izlenerek dokular parafin blok içine gömüldü. Dokulardan alınan kesitler 5 μm kalınlığında kesildi ve histopatolojik değişiklikleri saptamak için Hematoksilen & Eozin ile boyandı. Kesitler ışık mikroskobunda incelendi ve fotoğrafl arı çekildi. İmmünohistokimyasal boyama olarak Avidin-Biotin Peroksidaz yöntemi kullanıldı. Koyunların pulmoner adenokarsinomlarında tümör hücrelerinin asiner, papiller veya miks tip büyüme gösterdiği bulundu. Yirmi olgunun sadece 2’sinde lenf nodlarına metastaz vardı ve bu metastik alanlardaki tümöral hücre proliferasyonu, primer kanser odaklarına benzerdi. Tüm koyun pulmoner adenokarsinomları, JSRV CA ve Matriks Metalloproteinaz-9 immünoreaktivitesi yönünden pozitift i. Kontrol grubunda herhangi bir pozitif JSRV CA ve Matriks Metalloproteinaz-9 ekspresyonu tespit edilmedi. JSRV CA ve Matriks Metalloproteinaz-9 immün pozitif hücre sayısı, kontrol grubuna kıyasla koyun akciğer adenokarsinomu grubunda istatistiksel olarak artmıştı. Çalışmanın verileri doğrultusunda Matriks Metalloproteinazların başta Matriks Metalloproteinaz-9 olmak üzere koyun akciğer adenokarsinomlarında tümör metastazında ciddi rol oynadığı düşünülmektedir.

Th e Relationship Between Metastasis and MMP-9 in Sheep with Pulmonary Adenocarcinomas

Th is study aimed to evaluate the role of Matrix Metalloproteinase-9 in the metastasis of ovine pulmonary adenocarcinoma cases by immunohistochemical methods. Lung tissue samples were collected from 26 sheep brought to our department for routine histopathological diagnosis. Lung tissues were fixed in 10% neutral buff ered formalin, following routine procedures tissues were embedded in paraffin wax. Sections from tissues were cut into 5 μm thickness and stained with Hematoxylin & Eosin to detect histopathological changes. Th e sections were examined under a light microscope and photographs were taken. Avidin-Biotin Peroxidase method was used as immunohistochemical staining. Tumoral cells showed acinar, papillary or mixed type growths in ovine pulmonary adenocarcinomas. Only 2 of 20 cases had metastases to lymph nodes, and tumoral cell proliferation in these metastic areas was similar to primary cancer foci. All ovine pulmonary adenocarcinomas were positive for JSRV CA and Matrix Metalloproteinase-9 immunoreactivity. Any positive expression of JSRV CA and Matrix Metalloproteinase-9 were not detected in the control group. Th e number of JSRV CA and Matrix Metalloproteinase-9 immune positive cells was statistically increased in the ovine pulmonary adenocarcinoma group compared to the control group. In line with the data of this study, it is thought that Matrix Metalloproteinases play a serious role in tumor metastasis in ovine pulmonary adenocarcinomas, especially Matrix Metalloproteinase-9.

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