Ethem UNAL, Hafize UZUN, Abdullah YILDIZ, Berrin Papila KUNDAKTEPE, Zuhal Kus SILAV, Sinem DURMUŞ, Sema YUKSEKDAG, Remise GELISGEN

Akut Pankreatitli Sıçanlarda Bağırsak Geçirgenliğinin Değerlendirilmesinde Serum ve Bağırsak Dokusu Zonulin Düzeyleri

Deneysel akut pankreatitin (AP) histopatolojik bulguları ile zonulin düzeyleri ve oksidatif stres belirteçleri olan malondialdehit (MDA), süperoksit dismutaz (SOD), lipid hidroperoksit (LOOH) ve glutatyon (GSH) düzeyleri arasındaki ilişkinin değerlendirilmesi amaçlanmıştır. Bu çalışmaya, üç grup olacak şekilde Sprague-Dawley sıçanı (her grupta yedi hayvan) alınmıştır. Birinci grup kontrol grubu (sham laparotomi) olarak belirlendi. Grup 2 (%48 etil alkol) AP ve grup 3 (%80 etil alkol) ağır (şiddetli) pankreatit (ŞP) olarak tasarlandı. Pankreatitin etkileri, pankreas ve bağırsak dokusunda bu gruplar karşılaştırılarak histopatolojik sonuçlara göre polimorfonükleer lökositler, infiltrasyon, ödem, hemoraji, apoptoz, asiner hücresel dejenerasyon değerlendirildi. ŞP grubunda tamamen düzleşmiş mukozal yüzey ve ciddi villus kaybı (total villus atrofisi), lamina propriada kriptlerde dezorganizasyon ve hiperplazi tespit edildi. Serum ve bağırsak dokusunda MDA, LOOH ve zonulin düzeyleri kontrol grubuna göre ŞP grubunda anlamlı olarak yüksekti. Serum ve bağırsak dokusu SOD ve GSH değerleri kontrol grubuna göre anlamlı düşük bulundu. AP grubunda dokulardaki LOOH ve zonulin düzeyleri kontrol grubuna göre anlamlı yüksek bulunurken, sadece serum zonulin düzeyleri kontrole göre daha yüksekti. Zonulin ve oksidatif stres esas olarak pankreatit patogenezinde rol oynar. Biyokimyasal sonuçlar ayrıca bağırsak ve pankreas dokusundaki histopatolojik iyileşme ile de desteklenmektedir. Pankreatitli hastalar bozulmuş bağırsak bariyer fonksiyonuna daha fazla maruz kalabilirler. AP’de bağırsak geçirgenliğinin değerlendirilmesinde serum zonulin düzeyleri kullanılabilir.

Serum and Intestinal Tissue Zonulin Levels in the Evaluation of Intestinal Permeability in Rats with Acute Pancreatitis

To evaluate the eff ects on malondialdehyde (MDA), superoxide dismutase (SOD), lipid hydroperoxide (LOOH), glutathione (GSH) levels as oxidative stress markers, zonulin levels, and histopathological findings of experimental acute pancreatitis (AP). Th is study was conducted three groups of Sprague-Dawley rats (seven animals in every group). First group was evaluated as control group (sham laparotomy). Group 2 (AP, 48% ethyl alcohol) and group 3 (severe pancreatitis (SP), 80% ethyl alcohol) was performed. Th e eff ects of pancreatitis were evaluated by comparing these groups according to histopathologial results of polimorphonuclear leukocytes, infiltration, oedema, haemorrhagie, apoptosis, aciner cellular degeneration in pancreatic and intestinal tissue. In the SP group, completely fl attened mucosal surface and severe villi loss (total villous atrophy), disorganization and hyperplasia in the crypts in the lamina propria were detected. MDA, LOOH and zonulin levels in serum and intestinal tissue were found to be significantly higher in the SP group compared to the control group. Th e serum and intestinal tissue SOD and GSH were found to be significantly lower than the control group. In the AP group, while LOOH and zonulin in tissues were significantly higher than control, only zonulin levels in serum were found higher than control. Zonulin and oxidative stress is basically involved in the pathogenesis of pancreatitis. Biochemical results are also supported by histopatological improvement in intestinal and pancreas tissue. Patients with pancreatitis may be more exposed to impaired gut barrier function. Serum zonulin levels can be used in the evaluation of intestinal permeability in AP.

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