Davut KOCA, Mustafa AKKASOGLU, Hakan SAGIRKAYA, Mehmet Melih YILMAZ, Mehmet Ali KILIC, Ahmet AKTAR, Selim ALCAY

Otolog Trombositten Zengin Plazma Üreme Mevsimi Dışında Koç Spermatozoalarının Kriyoprezervasyonu Üzerinde Olumlu Etkiye Sahiptir

Bu çalışmanın amacı, otolog trombositten zengin plazma (PRP) ilave edilmiş yumurta sarılı sulandırıcılar eritme sonrası koç sperması üzerine etkilerini değerlendirmektir. Dokuz adet kıvırcık koçtan elde edilen sperma numuneleri dört eşit hacme bölündü ve farklı konsantrasyonlarda PRP içeren sulandırıcılar [PRP yok (Kontrol), 12.5x106/mL PRP, 25x106/mL PRP ve 50x106/mL PRP) ile sulandırıldı. Çözdürme sonrası motilite, plazma membranı fonsiyonel bütünlüğü, akrozomal bütünlük, mitokondriyal fonksiyon, DNA bütünlüğü ve malondialdehit konsantrasyonu (MDA) ölçüldü ve analiz edildi. Sonuç olarak, 25x106/mL PRP içeren sulandırcının çözdürme sonrası motilite (61.67±3.81), plazma membran fonksiyonel bütünlüğü (71.00±2.96), ve MDA seviyeleri (5.13±0.64) üzerine olumlu etkisi olduğunu gösterdi (P

Autologous Platelet Rich Plasma Have Positive Eff ect on Ram Spermatozoa During Cryopreservation in Non-Breeding Season

Th e aim of this study was to evaluate the eff ects of autologous platelet-rich plasma (PRP) supplemented egg yolk based extender on ram semen aft er thawing. Semen samples were collected from nine Kıvırcık rams and each semen was divided into four equal aliquots and mixed with diff erent concentrations of PRP supported extenders [no PRP (control), 12.5x106/mL PRP, 25x106/mL PRP, or 50x106/ mL PRP)]. Motility, plasma membrane functional integrity, acrosome integrity, mitochondrial membrane potential, DNA integrity and malondialdehyde concentrations (MDA) were measured and analyzed aft er thawing. Th e results showed that 25x106/mL PRP group had positive eff ect on motility (61.67±3.81), membrane functional integrity (71.00±2.96) and MDA levels (5.13±0.64) at post-thawed (P

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