Zekariya NUR, N. Tekin ÖNDER, Burcu ÜSTÜNER, Eser AKAL, Ahmet AKTAR, Oğuzhan HURAYDIN, Selim ALCAY, M. Melih YILMAZ, Elif GÖKÇE, Muhammed DUMAN

Gökkuşağı Alabalığı (Oncorhynchus mykiss) Seminal Plazması İlave Edilmiş Liyofilize Sulandırıcı, Koç Spermasının Dondurulmasını İyileştirir

Bu çalışma Gökkuşağı alabalığı seminal plazması (ASP) ilave edilmiş (kontrol, %10 ve %15) liyofilize sulandırıcının koç spermasının dondurulabilirliği üzerindeki etkisini belirlemeyi amaçlamıştır. Alınan sperma birleştirilerek, iki eşit hacime bölündü ve ejakülatların her biri, iki aşamalı sulandırma yöntemi kullanılarak ASP (%0, FC ve LC; %10 F10 ve L10 veya %15 F15 ve L15) ilave edilen taze ve liyofilize sulandırıcılarla sulandırıldı. Sperma programlanabilir dondurma makinesi kullanılarak donduruldu. Sperma numuneleri, taze ve eritme sonrası aşamada motilite, akrozom hasarı, plazma membran bütünlüğü ve DNA fragmentasyonu bakımından incelendi. Eritme sonrası en yüksek motilite ve plazma membrane bütünlüğü F10, F15 ve L15 gruplarında gözlendi (P

Lyophilized Extender Supplemented with Rainbow Trout (Oncorhynchus mykiss) Seminal Plasma Improves Cryopreservation of Ram Sperm

Th is study aimed to investigate the eff ect of the Rainbow trout seminal plasma (RTSP) supplemented (control, 10% or 15%) lyophilized extender on freezability of ram semen. Collected semen was pooled and split into two aliquots, and each of the ejaculates was diluted with fresh or lyophilized extenders with RTSP (0%, FC and LC; 10% F10 and L10or 15% F15 and L15) using two-step dilution method. Semen was frozen using the programmable freezing machine. Semen samples were examined for sperm motility, defective acrosomes, plasma membrane integrity and DNA fragmentation at native and post-thaw stage. Th e highest percentages of post-thaw motility and plasma integrity were observed in the F10, F15, and L15 (P

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1. Ustuner B, Gunay U, Nur Z, Ustuner H: Effects of long and short-term progestagen treatments combined with PMSG on oestrus synchronization and fertility in Awassi ewes during the breeding season. Acta Vet Brno, 76, 391-397, 2007. DOI: 10.2754/avb200776030391
2. Masoudi R, Zare Shahneh A, Towhidi A, Kohram H, Akbarisharif A, Sharafi M: Fertility response of artificial insemination methods in sheep with fresh and frozen-thawed semen. Cryobiology, 74, 77-80, 2017. DOI: 10.1016/j.cryobiol.2016.11.012
3. Nur Z, Zik B, Ustuner B, Sagirkaya H, Ozguden CG: Effects of different cryoprotective agents on ram sperm morphology and DNA integrity. Theriogenology, 73 (9): 1267-1275, 2010. DOI: 10.1016/j.theriogenology. 2009.12.007
4. Ustuner B, Alçay S, Nur Z, Sağırkaya H, Soylu MK: Effect of egg yolk and soybean lecithin on tris-based extender in post-thaw ram semen quality and in vitro fertility. Kafkas Univ Vet Fak Derg, 20 (3): 393-398, 2014. DOI: 10.9775/kvfd.2013.10248
5. Alcay S, Toker MB, Gokce E, Onder NT, Ustuner B, Nur Z: Long-term incubation resilience of post-thaw ram semen diluted with lecithin-based extender supplemented with bovine serum albumin. Kafkas Univ Vet Fak Derg, 25 (3): 291-297, 2019. DOI: 10.9775/kvfd.2018.20843
6. Pool KR, Rickard JP, De Graaf SP: Melatonin improves the motility and DNA integrity of frozen-thawed ram spermatozoa likely via suppression of mitochondrial superoxide production. Domest Anim Endocrinol, 74:106516, 2021. DOI: 10.1016/j.domaniend.2020.106516
7. Len JS, Koh WSD, Tan SX: The roles of reactive oxygen species and antioxidants in cryopreservation. Biosci Rep, 39 (8): BSR20191601, 2019. DOI: 10.1042/BSR20191601
8. Riesco MF, Alvarez M, Anel-Lopez L, Neila-Montero M, Palacin- Martinez C, Montes-Garrido R, Boixo JC, de Paz P, Anel L: Multiparametric study of antioxidant effect on ram sperm cryopreservation-from field trials to research bench. Animals, 11 (2): 283, 2021. DOI: 10.3390/ani11020283
9. Wu C, Dai J, Zhang S, Sun L, Liu Y, Zhang D: Effect of thawing rates and antioxidants on semen cryopreservation in Hu sheep. Biopreserv Biobank, 19 (3): 204-209, 2021. DOI: 10.1089/bio.2020.0067
10. Jha PK, Shahi Alam MG, Mansur AA, Naher N, Islam T, Uddin Bhuiyan M, Bari FY: Cryopreservation of Bangladeshi ram semen using different diluents and manual freezing techniques. Cryobiology, 89, 35-41, 2019. DOI: 10.1016/j.cryobiol.2019.06.001
11. Sarlos P, Molnar A, Kokai A, Gabor G, Ratky J: Evaluation of the effect of antioxidants in the conservation of ram semen. Acta Vet Hung, 50, 235- 245, 2002. DOI: 10.1556/avet.50.2002.2.13
12. Baran A, Ak K, Ileri IK, Soylu MK: Effects of adding bull seminal plasma to ram semen extenders on post-thaw spermatozoa motility and morphology. Indian Vet J, 81, 780-783, 2004.
13. Ustuner B, Alcay S, Toker MB, Nur Z, Gokce E, Sonat FA, Gul Z, Duman M, Ceniz C, Uslu A, Sagirkaya H, Soylu MK: Effect of rainbow trout (Oncorhynchus mykiss) seminal plasma on the post-thaw quality of ram semen cryopreserved in a soybean lecithin-based or egg yolk-based extender. Anim Reprod Sci, 164, 97-104, 2016. DOI: 10.1016/j.anireprosci.2015.11.017
14. Ustuner B, Gokce E, Toker MB, Onder NT, Soylu MK, Sagirkaya H, Nur Z: Effect of sperm pooling with seminal plasma collected in breeding or nonbreeding season on Saanen goat sperm cryosurvival. Andrologia, 50:e12968, 2018. DOI: 10.1111/and.12968
15. Ciereszko A, Glogowski J, Dabrowski K: Cryopreservation in aquatic species. In, Tiersch TR, Mazik PM (Eds): Biochemical Characteristics of Seminal Plasma and Spermatozoa of Freshwater Fishes. 20-48, World Aquaculture Society, USA, 2000.
16. Alcay S, Ustuner B, Aktar A, Mulkpınar, E, Duman M, Akkasoglu M, Cetinkaya M: Goat semen cryopreservation with rainbow trout seminal plasma supplemented lecithin-based extenders. Andrologia, 52:e13555, 2020. DOI: 10.1111/and.13555
17. Nynca J, Arnold G, Fröhlich T, Ciereszko A: Proteomic identification of rainbow trout blood plasma proteins and their relationship to seminal plasma proteins. Proteomics, 17 (11): 1600460, 2017. DOI: 10.1002/pmic. 201600460
18. Lahnsteiner F, Mansour N, Berger B: Seminal plasma proteins prolong the viability of rainbow trout (Oncorynchus mykiss) spermatozoa. Theriogenology, 62 (5): 801-808, 2004. DOI: 10.1016/j.theriogenology 2003.12.001
19. Lahnsteiner F, Patzner RA, Weismann T: Energy resources of spermatozoa of the rainbow trout Oncorhynchus mykiss (Pisces, Teleostei). Reprod Nutr Dev, 33, 349-360, 1993. DOI: 10.1051/rnd:19930404
20. Shaliutina-Kolešová A, Kotas P, Štěrba J, Rodina M, Dzyuba B, Cosson J, Linhart O: Protein profile of seminal plasma and functionality of spermatozoa during the reproductive season in the common carp (Cyprinus carpio) and rainbow trout (Oncorhynchus mykiss). Mol Reprod Dev, 83 (11): 968-982, 2016. DOI: 10.1002/mrd.22737
21. Zhao Z, Deng G, Lui Q, Laursen RA: Cloning and sequencing of cDNA encoding the LS-12 antifreeze protein in the longhorn sculpin, Myoxocephalus octodecimspinosis. Biochim Biophys Acta, 1382 (2): 177-180, 1998. DOI: 10.1016/s0167-4838(97)00197-0
22. Keskintepe L, Eroglu A: Preservation of Mammalian Sperm by Freeze- Drying. In, Wolkers WF, Oldenhof H (Eds): Cryopreservation and Freeze- Drying Protocols. Methods in Molecular Biology, 2180, 721-730, Humana, New York, NY. DOI:10.1007/978-1-0716-0783-1_39, 2021.
23. Glogowski J, Kwaoenik M, Piros B, Dabrowski K, Goryczko K, Dobosz S, Kuźmiński H, Ciereszko A: Characterization of rainbow trout milt collected with a catheter: Semen parameters and cryopreservation success. Aquac Res, 31, 289-296, 2000. DOI: 10.1046/j.1365-2109.2000.00400.x
24. Aisen E, Quıntana M, Medina V, Morello H, Venturino A: Ultramicroscopic and biochemical changes in ram spermatozoa cryopreserved with trehalose based hypertonic extenders, Cryobiology, 50, 239-249, 2005. DOI: 10.1016/j.cryobiol.2005.02.002
25. Alcay S, Toker MB, Gokce E, Ustuner B, Onder NT, Sagirkaya H, Nur Z, Soylu MK: Successful ram semen cryopreservation with lyophilized egg yolk-based extender. Cryobiology, 71 (2): 329-333, 2015. DOI: 10.1016/j. cryobiol.2015.08.008
26. Nur Z, Zık B, Ustuner B, Tutuncu S, Sagirkaya H, Ozguden CG, Gunay U, Dogan I: Effect of freezing rate of ram spermatozoa on acrosome and chromatin integrity. Ankara Univ Vet Fak Derg, 58, 267-272, 2011. DOI: 10.1501/Vetfak_0000002486
27. Buckett WM, Luckas MJ, Aird IA, Farquharson RG, Kingsland CR, Lewis-Jones DI: The hypo-osmotic swelling test in recurrentmiscarriage. Fertil Steril, 68, 506-509, 1997. DOI: 10.1016/s0015-0282(97)00241-0
28. Perez-Pe R, Barrios B, Muino-Blanco T, Cebrián-Pérez JA: Seasonal differences in ram seminal plasma revealed by partition in an aqueous twophase system. J Chromatogr B Biomed Sci Appl, 760 (1): 113-121, 2001. DOI: 10.1016/s0378-4347(01)00259-6
29. Abdelhakeam AA, Graham EF, Vazquez IA: Studies on the absence of glycerol in unfrozen and frozen ram semen: Fertility trials the effect of dilution methods on freezing ram semen in the absence of glycerol. Cryobiology, 28, 36-42, 1991. DOI: 10.1016/0011-2240(91)90005-9
30. Dcunha R, Hussein RS, Ananda H, Kumari S, Adiga SK, Kannan N, Zhao Y, Kalthur G: Current ınsights and latest updates in sperm motility and associated applications in assisted reproduction. Reprod Sci, 29 (1): 7-25, 2022. DOI: 10.1007/s43032-020-00408-y
31. Nynca J, Arnold GJ, Fröhlich T, Otte, K, Ciereszko A: Proteomic identification of rainbow trout sperm proteins. Proteomics, 14 (12): 1569- 1573, 2014. DOI: 10.1002/pmic.201300521
32. Jeyendran RS, Van Der Ven HH, Peez-Pelaez M, Crabo BG, Zaneveld LJ: Development of an assay to asses the functional integrity of the human sperm membrane and its relationship to other semen characteristics. J Reprod Fertil, 70 (1): 219-228, 1984. DOI: 10.1530/jrf.0.0700219
33. Holt WV, Penfold, LM: Animal andrology: Theories and applications. In, Chenoweth P, Lorton S (Eds): Fundamental and Practical Aspects of Semen Cryopreservation. 76-100, CAB International, UK, 2014. DOI: 10.1079/9781780643168.0076
34. Talukdar DJ, Ahmed K, Sinha S, Deori S, Das GC, Talukdar P: Cryopreservatiıon induces capacitation-like changes of the swamp buffalo spermatozoa. Buffalo Bull, 36 (1): 221-229,2017.
35. Abdelhakeam AA, Graham EF, Vazquez IA, Chaloner KM: Studies on the absence of glycerol in unfrozen and frozen ram semen: Development of an extender for freezing: effects of osmotic pressure, egg yolk levels, type of sugars and the method of dilution. Cryobiology, 28 (1): 43-49, 1991. DOI: 10.1016/0011-2240(91)90006-A
36. Savvulidi FG, Ptacek M, Malkova A, Beranek J, Stadnik L: Optimizing the conventional method of sperm freezing in liquid nitrogen vapour for Wallachian sheep conservation program. Czech J Anim Sci, 66, 55-64, 2021. DOI: 10.17221/226/2020-CJAS
37. Majzoub A, Agarwal A, Esteves SC: Clinical utility of sperm DNA damage in male infertility. Panminerva Med, 61 (2): 118-127, 2019. DOI: 10.23736/S0031-0808.18.03530-9
38. Seli E, Gardner DK, Schoolcraft WB, Moffatt O, Sakkas D: Extent of nuclear DNA damage in ejaculated spermatozoa impacts on blastocyst development after in vitro fertilization. Fertil Steril, 82, 378-383, 2004. DOI: 10.1016/j.fertnstert.2003.12.039
39. Silva PFN, Gadella BM: Detection of damage in mammalian sperm cells. Theriogenology, 65, 958-978, 2006. DOI: 10.1016/j.theriogenology. 2005.09.010