Mohadeseh PIRHADI, Vahab Hassan KAVIAR, Elahe KARIMI, Naser ABBASI, Ali AIDY, Mahmoud BAHMANI

Loranthus europaeus Jacq. Uçucu Yağ ve Özütünün Fitokimyasal Analizi ve Acinetobacter baumannii, Staphylococcus aureus ve Pseudomonas aeruginosa Üzerine Antimikrobiyal Etkisi

Antibiyotik kullanımının ve bunlara karşı direncin artmasıyla birlikte daha düşük direnç ve yan etki olasılığı olan doğal ilaçlara dikkat çekilmeye başlanmıştır. Bu çalışmada, Loranthus europaeus Jacq. uçucu yağı kimyasal olarak tanımlandı ve uçucu yağ ve ekstraktın Acinetobacter baumannii, Staphylococcus aureus ve Pseudomonas aeruginosa gibi bakteriyel etkenler üzerine antimikrobiyal etkileri araştırıldı. L. europaeus yaprakları Ilam dağlarından toplandı ve kurutulduktan sonra uçucu yağ ve hidroalkolik ekstrakt hazırlandı. L. europaeus bitkisinin uçucu yağı ve ekstraktının kimyasal bileşimleri, Headspace-katı faz mikroekstraksiyon (HS-SPME), Gaz kromatografisi-kütle spektrometrisi (GC-MS) ve Yüksek Performanslı Sıvı Kromatografisi (HPLC) yöntemleri ile ölçüldü. Sonuçlar, L. europaeus özütünün ana bileşeninin Rutin (223 μg/mL) olduğunu gösterdi. Bakteri suşları klinik örneklerden izole edildi ve minimum inhibitör konsantrasyonu (MIC) ve minimum bakterisidal konsantrasyonu (MBC) değerlendirmek için Klinik ve Laboratuvar Standartları Enstitüsü (CLSI) yöntemine göre mikro broth seyreltme yöntemi kullanıldı. L. europaeus yaprak özütünün MIC ve MBC değerleri, S. aureus için sırasıyla 6 μg/mL ve 196 μg/mL saptandı. A. baumannii ve P. aeruginosa suşları üzerine önemli bir etkisi olmadı. L. europaeus yaprağı esansiyel yağı, bakteri sosları üzerine antimikrobiyal etkiye sahip değildi. Sonuçlar, L. europaeus bitki özütünün, S. aureus’a karşı çok düşük MIC’i ile enfeksiyonları tedavi etmek için kullanılabileceğini gösterdi.

Phytochemical Analysis and Antimicrobial Eff ect of Essential Oil and Extract of Loranthus europaeus Jacq. on Acinetobacter baumannii, Staphylococcus aureus, and Pseudomonas aeruginosa

With the increase in the use of antibiotics and resistance against them, attention has been paid to natural remedies with the possibility of lower resistance and side eff ects. In this study, the essential oil of Loranthus europaeus Jacq. was identified chemically and the antimicrobial eff ects of the essential oil and extract on bacterial agents such as Acinetobacter baumannii, Staphylococcus aureus, and Pseudomonas aeruginosa were investigated. L. europaeus leaves were collected from the Ilam mountains and aft er drying, essential oil and hydroalcoholic extract were prepared. Th e chemical compositions of the essential oil and extract of L. europaeus plant were measured by Headspace-solid phase microextraction (HS-SPME), Gas chromatography-mass spectrometry (GC-MS), and High Performance Liquid Chromatography (HPLC) methods. Th e results showed that the main component of L. europaeus extract was Rutin (223 μg/mL). Th e bacterial strains were isolated from clinical samples, and the microbroth dilution method by Clinical and Laboratory Standards Institute (CLSI) method were used to evaluate the Minimum inhibitory concentration (MIC) and Minimum Bactericidal Concentration (MBC). Th e MIC and MBC for the L. europaeus leaf extract were 6 μg/mL and 196 μg/mL on S. aureus, respectively. It had no significant eff ect on the strains of A. baumannii and P. aeruginosa. L. europaeus leaf essential oil also had no antimicrobial eff ect on bacterial sauces. Th e results showed that the plant extract of L. europaeus might be used to treat infections due to its very low MIC against S. aureus.

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