UĞUR SIDAL, EBRU ŞEBNEM YILMAZ

Production of rhamnolipid (a biosurfactant) using free and immobilized cells of Pseudomonas sp.

Bu çalışmada, bir büyosürfektan olan ramnolipidin Pseudomonas sp.’den üretimi araştırılmıştır. Nutrient agarda üretilen Pseudomonas sp. örnekleri sıvı besiyerine aktarılmıştır. 24 saatlik inkübasyon sonrası, 2 ml’lik kültür örnekleri ramnolipid üretimi için, farklı bir sıvı besiyerine aktarılarak 26. saatte sonuçlar gözlenmiştir. Çalışmada, ayrıca ramnolipid üretimine sıcaklığın, pH’ın ve glukoz konsantrasyonunun etkisi de incelenmiştir. İmmobilize (tutuklanmış) hücreler içinde aynı metotlar uygulanmıştır. Ramnolipid üretimi, serbest hücrelerde tutuklanmış hücrelerden daha yüksek bulunmuştur. Serbest hücreler, etkin ramnolipid üretimi için kullanılabilirler.

Anahtar Kelimeler:

Pseudomonas, glukoz, lipit, pH, sıcaklık

Serbest ve tutuklanmış Pseudomonas sp. hücrelerinden ramnolipid (biyosürfektan) eldesi

This study presents a method for the production of rhamnolipid, a biosurfactant, by Pseudomonas sp. Pseudomonas sp. cells that were grown in nutrient agar were inoculated into sterile liquid medium. Following an incubation period of 24 h, 2 ml of cells were inoculated into a different liquid medium and the results were obtained at the end of 26 hours incubation time. In our study, the effects of temperature, pH, and glucose concentration on rhamnolipid production were also investigated. Later, the same procedure was applied to immobilized cells that were kept away from the free microorganisms. The production of rhamnolipid by free cells was found to be much higher than that of immobilized cells. Free cells could be used for rhamnolipid production effectively.

Keywords:

Pseudomonas, glucose, lipids, pH, temperature,

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