DİLEK ÖZTÜRK, MEHMET KALE, FARUK PEHLİVANOĞLU, SİBEL HASIRCIOĞLU, Hülya TÜRÜTOĞLU

Evaluation for some bacterial and viral abortions of dairy cattle farms in burdur district of Turkey

Bu çalışmada, Türkiye’nin güneybatı bölgesinde yer alan Burdur ili’nde yavru atma problemli süt sığırlarında Brucella abortus, Chlamydophila abortus, Coxiella burnetii, Bovine herpesvirus-1 (BHV-1), Bovine viral diarrhea virus (BVDV) ve Bovine herpesvirus-4 (BHV-4)’e karşı antikorların varlığı araştırıldı. Kan örnekleri araştırılan enfeksiyonlara karşı aşılanmamış 10 sürüden 2 yaş ve üzeri 932 hayvandan toplandı. Serumlar önce B. abortus yönünden ELISA ile test edildi. B. abortus negatif bulunan serum örnekleri C. burnetii ve C. abortus’a karşı antikorların varlığı yönünden araştırıldı. B. abortus ve C. burnetii için pozitiflik sırasıyla %25.3 (236/932) ve %10.2 (19/186) belirlenirken, C. abortus antikorları tespit edilemedi. B. abortus, C. burnetii ve C. abortus negatif bulunan sürülerde BHV-1, BVDV ve BHV-4’e karşı antikorların varlığı araştırıldı. Seropozitiflik bu hastalıklar için sırasıyla %43.5 (40/92), %81.5 (75/92), %42.4 (39/92)olarak belirlendi. BVDV antijen ise örneklerin %2.2’sinde tespit edildi. Sonuç olarak, C. abortus’un Burdur’da süt sığırı işletmelerinde aborta neden olan önemli bir ajan olmadığı, B. abortus, C. burnetii, BHV-1, BVDV ve BHV-4’ün seropozitifliğinin yüksek olduğu belirlendi. Bu nedenle, sürülerin bu enfeksiyonlar yönünden düzenli olarak test edilmesi ve gerekli kontrol önlemlerinin alınması gerektiği sonucuna varıldı.

Anahtar Kelimeler:

viral hastalıklar, antikor, yavru atma, Türkiye, ELISA, Coxiella burnetii, Brucella abortus, sığır herpes virüsü 1, inek, süt sığırı, süt ineği, süt sığırcılığı, bakteriyel hastalıklar

Burdur yöresinde süt sığırı işletmelerinin bazı bakteriyel ve viral abortlar yönünden değerlendirilmesi

In this study, the presence of antibodies to Brucella abortus, Chlamydophila abortus, Coxiella burnetii, Bovine herpesvirus-1 (BHV-1), Bovine viral diarrhea virus (BVDV) and Bovine herpesvirus-4 (BHV-4) were investigated in dairy cattle herds with abortion history in Burdur province, Southwest region of Turkey. The blood samples were collected from 932 dairy cattle ≥2 years of age from 10 herds not vaccinated against for mentioned infections and all of the serum samples were tested for B. abortus by ELISA. Seronegative herds for B. abortus were investigated for C. burnetii and C. abortus. While seropositivity for B. abortus and C. burnetii were detected 25.3% (236/932) and 10.2% (19/186), antibodies against C. abortus was not detected. Seronegative herds for B. abortus, C. burnetii and C. abortus were analysed for antibodies to BHV-1, BVDV and BHV-4 and seropositivity were found as 43.5% (40/92), 81.5% (75/92), 42.4% (39/92), respectively. BVDV antigen was determined in 2.2% (2/92) of the samples. As a result, we determined that C. abortus is not an important agent causing abortion in dairy herds in Burdur, but the seropositivity of B. abortus, C. burnetii, BHV-1, BVDV and BHV-4 are high. Thus, the herds should be tested for these infections regularly and control measures must be taken.

Keywords:

viral diseases, antibodies, abortion, Turkey, ELISA, Coxiella burnetii, Brucella abortus, bovine herpesvirus 1, cows, dairy cattle, dairy cows, dairy herds, bacterial diseases,

PDF

___

1. Anderson ML, Blanchard PC, Barr BC, Hoffman RL: A survey of causes of bovine abortion occuring in the San Joaquin Valley, California. J Vet Diagn Invest, 2, 283-287, 1990.
2. Jamaluddin AA, Case JT, Hird DW, Blanchard PC, Peauroi JR, Anderson ML: Dairy cattle abortion in California: Evaluation of diagnostic laboratory data. J Vet Diagn Invest, 8, 210-218, 1996.
3. Corbellini LG, Pescador CA, Frantz F, Wunder E, Steffen D, Smith DR, Driemeier D: Diagnostic survey of bovine abortion with special reference to Neospora caninum infection: Importance, repeated abortion and concurrent infection in aborted fetuses in Southern Brazil. Vet J, 172, 114- 120, 2006.
4. Anderson ML: Diagnosis of infectious causes of bovine abortion. American Association of Bovine Practitioners. Conference, The AABP Proceedings, Sep. 23-Sep. 25, 37, 90-96. USA, 2004.
5. Khodakaram-Tafti A, Ikede BO: A retrospective study of sporadic bovine abortions, stillbirths, and neonatal abnormalities in Atlantic Canada, from 1990 to 2001. Can Vet J, 46, 635-637, 2005.
6. Denizli AN, Gokcen S, Ergun A: Ege bölgesi sığırlarında brucella enfeksiyonunun ELISA testi ile insidensinin saptanması. Hayvan Aşıları Kontrol Merkezi Müdürlüğü, 16, 48-57, 1992.
7. Iyisan S, Akmaz O, Duzgun SG, Ersoy Y, Eskiizmirliler S, Guler L, Gunduz K, Isik N, Icyerioglu AK, Kalender H, Karaman Z, Kucukayan U, Ozcan C, Seyitoglu S, Tuna I, Tunca T, Ustunakin K, Yurtalan S: Türkiye’de sığır ve koyunlarda Brucellosis’in epidemiyolojisi. Pendik Vet Mikrobiyol Derg, 31, 21-75, 2000.
8. Trangadia B, Rana SK, Mukherjee F, Srinivasan VA: Prevalence of brucellosis and infectious bovine rhinotracheitis in organized dairy farms in India. Trop Anim Health Prod, 42, 203-207, 2010.
9. Ibrahim N, Belihu K, Lobago F, Bekana M: Sero-prevalence of bovine brucellosis and its risk factors in Jimma zone of Oromia Region, South- western Ethiopia. Trop Anim Health Prod, 42, 35-40, 2010.
10. Demirozu K, Celik M, Iyisan S: Kars ilinde brusellosisin sero- epidemiyolojisi. Pendik Vet Mikrobiyol Derg, 24, 123-132, 1993.
11. Pehlivanoglu F, Ozturk D, Gunlu S, Guldali Y, Turutoglu H: Prevalence of Brucellosis in dairy herds with abortion problems. Kafkas Univ Vet Fak Derg,17 (4): 615-620, 2011.
12. Peter AT: Abortions in dairy cows: New insights and economic impact. Adv Dairy Sci Tech, 12, 233-244, 2000.
13. Turutoglu H, Iyisan AS, Duru A, Altınel C: Koyunlarda Chlamydia psittaci infeksiyonunun mikrokomplement fikzasyon testi ile saptanması. Pendik Vet Mikrobiyol Derg 26, 67-78, 1995.
14. Ozturk M, Nadas UG, Turutoglu H: İstanbul bölgesindeki koyunlarda Chlamydia psittaci infeksiyonunun prevalansının saptanması. Pendik Vet Mikrobiyol Derg, 29, 72-80, 1998.
15. Gokce HI, Kacar C, Genc O, Sozmen M: Seroprevalance of Chlamydophila abortus in aborting ewes and dairy cattle in the North east part of Turkey. B Vet I Pulawy, 51, 9-13, 2007.
16. Godin AC, Bjorkman C, Englund S, Johansson KE, Niskanen R, Alenius S: Investigation of Chlamydophila spp. in dairy cows with reproductive disorders. Acta Vet Scan, 50, 39, 2008.
17. Vlahovic K, Dovc A, Zupancic Z, Pavlaki M, Jercici J, Marina P, Jure J: Comparison of serological procedures for diagnosis of infection with Chlamydophila spp. in bovines. Vet Arhiv, 71, 67-379, 2001.
18. Greco G, Corrente M, Buonavoglia D, Campanile G, Di Palo R, Martella V, Bellacicco AL, Abramo MD, Buonavoglia C: Epizootic abortion related to infections by Chlamydophila abortus and Chlamydophila pecorum in water buffalo (Bubalus bubalis). Theriogenology, 69, 1061-1069, 2008.
19. Ozyer M, Mirioglu M, Koksal F: Çukurova bölgesinde yaşayan insan ve hayvanlarda Q-Fever infeksiyonu insidansının komplement fikzasyon testi ile karşılaştırılması. Pendik Hayvan Hast Mer Aras Enst Derg, 21, 28-39, 1990.
20. Seyitoglu S, Ozkurt Z, Dinler U, Okumus B: The seroprevalance of Coxiellosis in farmers and cattle in Erzurum district in Turkey. Turk J Vet Anim Sci, 30, 71-75, 2006.
21. Ceylan E, Berktas M, Keles I, Agaoglu Z: Seroprevalance of Q Fever in cattle and sheep in the East of Turkey. Asian J Anim Adv, 4, 114-121, 2009.
22. Kirkan S, Kaya O, Tekbiyik S, Parin U: Detection of Coxiella burnetii in cattle by PCR. Turk J Vet Anim Sci, 32, 215-220, 2008.
23. Kelly PJ, Matthewman LA, Mason, PR, Raoult D: Q fever in Zimbabwe. A review of the disease and the results of a serosurvey of humans, cattle, goats and dogs. S Afr Med J, 83, 21-25, 1993.
24. Serbezov V, Kazar J, Novkirishki V, Gatcheva N, Kovacova E and Voynova V: Q fever in Bulgaria and Slovakia. Emerg Infect Dis, 5, 388-394, 1999.
25. Cabassi CS, Taddei S, Donofrio G, Ghidini F, Piancastelli C, Flammini CF, Cavirani S: Assocation between Coxiella burnetii seropositivity and abortion in dairy cattle of Northern Italy. New Microbiol, 29, 211-214, 2006.
26. Medici KC, Alfieri AA, Alfieri AF: Prevalence of neutralizing antibodies against bovine herpesvirus Type 1, due natural infection, in herds with reproductive problems. Ciência Rural, 30, 347-350, 2000.
27. Dechicha A, Gharbi S, Kebbal S, Chatagnon G, Tainturier D, Ouzrout R, Guetarni D: Serological survey of etiological agents associated with abortion in two Algerian dairy cattle breeding farms. J Vet Med Anim Health, 2, 1-5, 2010.
28. Rüfenacht J, Schaller P, Audige L, Knutti B, Kupfer U: The effect of infection with bovine viral diarrhea virus on the fertility of swiss dairy cattle. Theriogenology, 56, 199-210, 2001.
29. Hall CA, Reichel MP, Ellis JT: Neospora abortions in dairy cattle: Diagnosis, mode of transmission and control. Vet Parasitol, 128, 231-241, 2005.
30. Naeem K, Goyal SM, Werdin RE: Prevalence of bovid herpesvirus and its antibody in cattle in Minnesota. Am J Vet Res, 50, 1931-1935, 1989.
31. Frazier KS, Baldwin CA, Pence M, West J, Bernard J, Liggett A, Miller D, Hines II ME: Seroprevalence and comparison of isolates of endo- metriotropic Bovine Herpesvirus-4. J Vet Diagn Invest, 14, 457-462, 2002.
32.Kale M, Ata A, Kocamüftüoglu M, Hasırcıoglu S: Bovine herpesvirus type 4 (BHV-4) infection in relation to fertility in repeat breeder dairy cows. Acta Vet Beo, 61, 13-19, 2011.
33. Mineo TWP, Alenius S, Naslund K, Montassier HJ, Bjorkman C: Distribution of antibodies against Neospora caninum, BVDV and BHV-1 among cows in Brazilian dairy herds with reproductive disorders. Rev Bras Parasitol Vet, 15, 188-192, 2006.
34. Bilge Dagalp S, Demir AB, Gungor E, Alkan F: The seroprevalence of Bovine Herpes Virus Type 4 (BHV4) infection in dairy herds in Turkey and possible interaction with reproductive disorders. Rev Med Vet-Toulouse, 158, 201-205, 2007.
35. Dhami JS, Dwivedi PN, Ramneek DD, Maiti NK, Oberoi MS: Diagnosis of bovine herpesvirus-1 (BHV-1) infection in aborting cattle and buffaloes by virus isolation and polymerase chain reaction (PCR). Indian J Anim Sci, 78, 1235-1237, 2008.
36. Kale M, Hasircioglu S, Acar A: Gebeliginin 5.-7. ayları arasında abort yapmıs süt sıgırlarında Bovine Herpesvirus-1, Bovine Viral Diarrhea Virus ve Bovine Herpesvirus-4 enfeksiyonlarının araştırılması. VIII. Ulusal Veteriner Mikrobiyoloji Kongresi (Uluslararası Katılımlı), 07-09 Ekim, Kampus - Van, Türkiye, s. 218-219, 2008.
37. Yıldırım Y, Yılmaz V, Kalaycıoğlu AT, Dağalp SB, Farajı Majarashın AR, Çelebi Ö, Akça D: An investigation of a possible involvement of BVDV, BHV-1 and BHV-4 infections in abortion of dairy cattle in Kars district of Turkey. Kafkas Univ Vet Fak Derg, 17, 879-883, 2011.
38. Meyling A, Houe H, Jensen AM: Epidemiology of bovine virus diarrhoea virus. Rev Sci Tech Off Int Epiz, 9, 75-93, 1990.
39. Heuer C, Healy A, Zerbini C: Economic effects of exposure to bovine viral diarrhea virus on dairy herds in New Zealand. J Dairy Sci, 90, 5428- 5438, 2007.
40. Norton JH, Tranter WP, Campbell RSF: A farming systems study of abortion in dairy cattle on the Atherton Tableland: 2. The pattern of infectious diseases. Aust Vet J, 66, 163-167, 2008.
41. Escamilla HP, Juan Martinez MJ, Mario Medina C, Elizabeth Morales S: Frequency and causes of infectious abortion in a dairy herd in Queretaro, Mexico. Can J Vet Res, 71, 314-317, 2007.
42. Caldow GL: Bovine abortion outbreak associated with Neospora and other infectious agents. Vet Rec, 142, 118-119, 1998.