TÜLİN ARSLAN, FATİME ERDOĞAN, METE ERDOĞAN

Fonksiyonel erkekleştirilmiş gökkuşağı alabalığı (oncorhynchus mykiss) üretimi

Bu çalışmada, sperm kanalları olan, abdominal masaj yoluyla gamet toplanabilen, fonksiyonel olarak erkekleştirilmiş gökkuşağı alabalığı (Oncorhynchus mykiss) anacı üretimi için uygun hormonal cinsiyet dönüşüm prosedürü araştırılmıştır. Bu amaçla 4 grup deneme yapılmıştır. Oral uygulama grubunda, serbest yüzmeye başlayan post larvalar ilk yem alma aşamasından itibaren 600 derece-gün süresince kilogramında 1.0-3.0 mg 17α-metiltestosteron içeren yemlerle beslenilmiştir. Banyo uygulama gruplarında ise, 0.5-1.0 mg l-1 17α-metiltestosteron, 11β-hidroksiandrostenedion ya da 17α-metildihidrotestosteron keseli larval dönem boyunca (döllenmeden itibaren 370-560 derece-gün) kısa süreli (2 saatlik) tek, 48 saat aralı 2-3 ya da 1 hafta aralı çift banyo şeklinde verilmiştir. Çalışma sonuçları, gökkuşağı alabalığında fonksiyonel erkekleştirilmiş dişi anaç üretimi için en uygun hormon prosedürünün döllenmeden sonraki 470-800 derece-gün’leri kapsayan, periyodik banyolar veya periyodik banyolar ve düşük dozda oral uygulamalar şeklinde bir prosedür olabileceğini, androjen banyolarının fonksiyonel erkekleştirilmiş dişi anaçlar üretmekte daha etkili olduğunu, düşük konsantrasyonlarda (≤1 mg kg-1 yem) verildiğinde oral 17α-metiltestosteron uygulamalarının da yüksek oranda fonksiyonel erkek birey üretebileceğini göstermiştir.

Anahtar Kelimeler:

diyetler, alabalık, testosteron, spermatozoa, gökkuşağı alabalığı, metodoloji, erkek hayvanlar, larva, balık beslenmesi

The production of functional sex-reversed male rainbow trout (oncorhynchus mykiss)

In this study, appropriate hormonal sex reversal procedure for the production of functional sex-reversed male rainbow trout (Oncorhynchus mykiss) brooders with intact sperm ducts was investigated. For this purpose, 4 groups of experiment were conducted. In oral administration group, free swimming larvae starting from the first feeding were fed 1.0-3.0 mg 17α-methyltestosterone kg-1 diets for 600 degree-days. In the remaining experimental groups, 0.5-1.0 mg l-1 17α-methyltestosterone, 11β-hidroksiandrostenedione or 17α-methyldihydrotestosterone were administered throughout the sac fry stage (370-560 degree-days post fertilization) as short (2 h) single, 48 h apart 2-3 or 1 week apart double baths. Results of the study demonstrated that appropriate androgen procedure for the production of functionally masculinized rainbow trout females should be covering the 470-800 degree-days post fertilization, but it could be periodic baths or periodic baths and oral administration of low concentrations, at low concentrations (≤1 mg kg-1 feed) oral administration of 17α-methyltestosterone could yield high ratios of functional sex reversed males.

Keywords:

diets, trout, testosterone, spermatozoa, rainbow trout, methodology, male animals, larvae, fish feeding,

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