Ayşegül ÇEBİ, FATİH ÇAĞLAR ÇELİKEZEN, ALİ ERTEKİN

Effects of coenzyme q10 on the levels of some trace elements in serum of rats with bleomycin ınduced pulmonary fibrosis

Koenzim Q10 (CoQ10) mitokondrial matrikste bulunan antioksidan bir elektron taşıyıcısıdır.Bu çalışmanın amacı bleomisin uygulanarak oluşturulmuş akciğer fibrozisinde CoQ10’ nin Fe, Cu, Zn ve Mn düzeyleri üzerine koruyucu etkisini araştırmaktı. Otuz Wistar albino ırkı rat yalnız bleomisin uygulanan grup, bleomisin + CoQ10 uygulanan grup, ve yalnız serum fizyolojik uygulanan grup (kontrol grubu) olmak üzere 3 gruba ayrıldı. Bleomisin grubuna bleomisin hidroklorid 7.5 mg/kg canlı ağırlık oranında intratrakeal olarak tek doz şeklinde uygulandı. Bleomisin + CoQ10 grubuna da bleomisine ilave olarak haftada iki kez CoQ10 uygulandı. Kontrol grubuna ise yalnız serum fizyolojik uygulandı. Bleomisin uygulamasından 14 gün sonra ratlara ötenazi uygulandı. Serumlarında Fe, Cu, Zn ve Mn düzeyleri saptandı. Fe, Cu, Zn ve Mn düzeylerinin belirlenmesinde atomik absorbsiyon spektrofotometresi kullanıldı. Elde ettiğimiz veriler, bleomisinin antioksidan sistemde önemi olan Fe, Cu, Zn ve Mn dengesini bozduğunu ve CoQ10’ in akciğer fibrozisinde ağır metal ve iz element dengesizliğini yeniden değiştirdiğini düşündürmektedir.

Anahtar Kelimeler:

demir, sıçan, çinko, ubikuinon, eser elementler, mangan, akciğerler, fibroz, bakır, kan kimyası

Koenzim q10’ nin bleomisinin teşvik ettiği akciğer fibrozisli ratların serumlarındaki bazı iz elementlerin seviyelerine etkisi

Coenzyme Q10 (CoQ10) is an antioxidant and an electron carrier in the mitochondrial matrix. The aim of this study was to study the protective effect of CoQ10 on the levels of Fe, Cu, Zn and Mn in serum with bleomycin induced pulmonary fibrosis in rats. Thirty Wistar albino rats were randomly divided into three groups: bleomycin alone, bleomycin + CoQ10, and physiological saline alone (control group). The bleomycin group was given 7.5 mg/kg body weight (single dose) bleomycin hydrochloride intratracheally. The bleomycin + CoQ10 group was also instilled with bleomycin hydrochloride but received administrations of CoQ10 twice a week. The control group was treated with physiological saline alone. Animals were euthanized 14 d after intratracheal instillation of bleomycin. Fe, Cu, Zn and Mn were measured in the serum of rats. Atomic absorption spectroscopy was used to measure the Fe, Cu, Zn and Mn levels. Our data may suggest that bleomycin impairs the equilibrium of Fe, Cu, Zn and Mn which are important in the antioxidant system and CoQ10 tends to rechange the imbalance of some trace elements in pulmonary fibrosis.

Keywords:

iron, rats, zinc, ubiquinones, trace elements, manganese, lungs, fibrosis, copper, blood chemistry,

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