Siriwadee CHOMDEJ, Pollawath LEELAWATTANAKUL, Kittisak BUDDHACHAT, Waranee PRADİT, Puntita SIENGDEE, Kannika PHONGROOP, Korakot NGANVONGPANIT

Preliminary Study on Association of EDNRB Gene with Heterochromia Iridis in Cats (Felis catus)

This study conducted an investigation on three exons of the endothelin receptor type B (EDNRB) gene of Thai odd-eyed cats to find out the association between the variations in the gene and heterochromia iridis. DNA sequencing analysis was performed on 11 odd-eyed cats compared to 11 normal-eyed cats. Seven variations were found across the studied region (XM_003980457.2: c.610A>G, c.820+40C>T, c.821-14C>T, c.916A>G, c.1025+36G>T, c.1025+69A>T, and c.1025+138C>T) with two of them (c.610A>G and c.916A>G) causing amino acid changes (P.Asn128Ser and P.Val230Ala). There was no statistical association between the variations near the three exons of EDNRB and feline heterochromia iridis (chi-square test, P-value >0.05).

Kedilerde (Felis catus) EDNRB Geni ile Heterokromia İridis Arasında İlişkiye Dair Ön Çalışma

Bu çalışmada Tayland tek-göz kedilerinde endotelin reseptör tip B (EDNRB) geninin üç ekzonunda gen varyasyonu ile heterokromia iridis arasında bir ilişki olup olmadığı araştırıldı. On bir tek-göz kedide DNA sekans analizi yapıldı ve 11 normal gözlü kedi ile karşılaştırıldı. Çalışılan bölge itibarıyla yedi varyasyon (XM_003980457.2: c.610A>G, c.820+40C>T, c.821-14C>T, c.916A>G, c.1025+36G>T, c.1025+69A>T ve c.1025+138C>T) tespit edildi ve bunların ikisi (c.610A>G ve c.916A>G) amino asit değişimine (P.Asn128Ser ve P.Val230Ala) neden olmaktaydı. Çalışmada, üç EDNRB ekzonu yakınındaki varyasyonlar ile kedi heterokromia iridis arasında istatistiksel bir ilişki tespit edilmedi (ki kare testi, P-değeri >0.05).

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