ÖZLEM DEMİRCİ TURGUNBAYER, NESRİN HAŞİMİ, ERSİN KILINÇ, VEYSEL TOLAN

Evaluation of Genotoxic Effects of C60 Fullerene-γ-Fe2O3 and MultiWall Carbon Nanotubes-γ-Fe2O3 Nanoparticles

The use of magnetic nanoparticles in nanomedicine applications has increased significantly in recent years. Genotoxic evaluation of the nanomaterials used for this purpose is therefore very important. In our study, the genotoxic effect of C60 fullerene-γ-Fe2O3 and multi-wall carbon nanotubes-γ-Fe2O3 magnetic nanoparticles over a wide concentration range (0.1, 1.0, 5.0, 10.0, 25.0, 50.0, and 100.0 µg/plate) was investigated using the Bacterial Reverse Mutation Test. These magnetic nanoparticles did not cause genetic damage to Salmonella typhimurium TA100 and TA98 in the presence and absence of metabolic activation. Due to the rapid increase in the presence of nanoparticles in our daily lives, mutagenicity and toxicity data related to nanoparticles are quite valuable. For this reason, in vivo and in vitro studies that allow for effective evaluation of these compounds is of the utmost importance.

C60 Fullerene-γ-Fe2O3 ve Çok Duvarlı Karbon Nanotüpler-γ-Fe2O3 Nanopartiküllerinin Genotoksik Etkilerinin Değerlendirilmesi

Nanotıp uygulamalarında manyetik nano parçacıkların kullanılması son yıllarda önemli ölçüde artmıştır. Bu amaçla kullanılan nano malzemelerin genotoksik değerlendirilmesi bu nedenle çok önemlidir. Çalışmamızda, C60 fullerene-γ-Fe2O3 ve çok duvarlı karbon nanotüpler γ-Fe2O3 manyetik nanopartiküllerin geniş bir konsantrasyon aralığında (0.1, 1.0, 5.0, 10.0, 25.0, 50.0 ve 100.0 µg/plaka) genotoksik etkisi Bakteriyel Geri Mutasyon Testi kullanılarak araştırıldı. Bu manyetik nanopartiküller, metabolik aktivasyonun varlığında ve yokluğunda Salmonella typhimurium TA100 ve TA98’de genetik hasara neden olmamıştır. Günlük yaşamlarımızda nanopartiküllerin varlığındaki hızlı artış nedeniyle, nanopartiküller ile ilgili mutajenite ve toksisite verileri oldukça değerlidir. Bu nedenle, bu bileşiklerin etkili bir şekilde değerlendirilmesine olanak sağlayan in vivo ve in vitro çalışmalar oldukça önemlidir.

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