Metin ÖĞÜN, Mushap KURU, Abdulsamed KÜKÜRT, Recai KULAKSIZ, Hasan ORAL

Controlled Internal Drug Release Uygulaması İle Senkronizasyon Yapılan Gebe ve Gebe Kalmayan Abaza Keçilerinde Oksidatif/Nitrozatif Stres, Leptin ve Progesteron Konsantrasyonlarının Karşılaştırılması

Bu çalışmanın amacı üreme sezonunda controlled internal drug release (CIDR) ile senkronizyon sonrasında gebe ve gebe olmayan Abaza keçilerinde oksidatif/nitrosatif stres, leptin ve progesteron konsantrasyonlarının belirlenmesidir. Bu amaçla, klinik olarak sağlıklı 2-4 yaşlı 40 Abaza keçisine 0. gün CIDR intravaginal olarak yerleştirildi ve 9. gün equine chorionic gonadotropin ile prostaglandin F2α enjekte edildi. CIDR 11. gün çıkarıldı. Uygulama sonrasında östrus takibi yapılarak keçiler fertil tekeler ile çiftleştirildi. Senkronizasyona başlamadan 8 gün önce, 0. gün, 11. gün ve çiftleşme günü kan alındı. Çiftleşmeden sonraki 30. gün transrektal ultrasonografi ile gebelik muayenesi yapıldı. Biyokimyasal ölçümler için 40 keçiden (30 gebe + 10 gebe olmayan) elde edilen kan serumu kullanıldı. Gebe olan keçilerle gebe olmayan keçiler karşılaştırıldığında malondialdehid (MDA), nitrik oksit (NO), total oksidan kapasite (TOC), total antioksidan kapasite (TAC) ve endotelyal NO sentaz (eNOS) aktiviteleri anlamlı olarak yüksek bulundu (P

Comparison of Oxidative/Nitrosative Stress, Leptin and Progesterone Concentrations in Pregnant and Non-pregnant Abaza Goats Synchronized with Controlled Internal Drug Release Application

The aim of this study was to determine the oxidative/nitrosative stress, leptin and progesterone concentrations in pregnant and non-pregnantAbaza goats after synchronization with controlled internal drug release (CIDR) during the breeding season. For this purpose, 40 clinicallyhealthy Abaza goats, aged 2-4 years, were intravaginally exposed to CIDR on day 0, and injected with equine chorionic gonadotropin andprostaglandin F2α on day 9 of the experiment. CIDR was removed on day 11. At the end of the experiment, they were monitored for estrusand exposed to fertile males for mating. Blood samples were collected 8 days before synchronization, then on days 0, 11 of CIDR insertionand on mating day. A pregnancy diagnosis was conducted using transrectal ultrasonography 30 day after mating. The blood serum from 40goats (30 pregnant + 10 non-pregnant) was used for biochemical analyses. Malondialdehyde (MDA), nitric oxide (NO), total oxidant capacity(TOC), total antioxidant capacity (TAC) and endothelial NO synthase (eNOS) activities were significantly higher on mating day in pregnantgoats compared to non-pregnant goats (P

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