Jorge MIRANDA, Salim MATTAR, Carmen GALVÁN, Juan BALLUT

Kolombiya, Cordoba’daki Köpeklerde Babesia spp.

Köpek babesiosisi (Canine babesiosis), Babesia cinsinin intraeritrositik protozoası tarafından oluşturulan ve dünya çapında yaygınlık gösteren kene kaynaklı bir hastalıktır. Bu çalışmada, Córdoba, Kolombiya’daki köpeklerde B. canis vogeli’nin moleküler tespiti ve hastalığın klinik özellikleri tanımlanmıştır. Kene kaynaklı hastalıklarla uyumlu klinik bulguları olan köpeklerden kan örnekleri alındı. Periferal kan örnekleri Babesia spp. merozoit ve trofozoitlerinin mevcudiyetini belirlemek amacıyla mikroskopik olarak incelendi. DNA ekstraksiyonu için juguler veya sefalik venipunktür ile elde edilen kan örnekleri kullanıldı. PCR analizi, Babesia spp. 18S rRNA genine yönelik primerler kullanılarak gerçekleştirildi. Babesiosis şüphesi olan 42 köpeğin 23’ü dişi ve 19’u erkek idi. Köpeklerin 42’sinin 23’ünde (%54.7), periferik kan muayenesinde Babesia spp. ile uyumlu intraeritrositik piroplazmalar saptandı. Morfometrik çalışma, %73’ünün (17/23) büyük Babesia (2.4x5 µm) ve %26’sının (6/23) küçük Babesia (1x3.2 µm) olduğunu göstermiştir. Kan örneklerinin 42’sinden 11’i B. canis vogeli için pozitif idi. Pozitif köpeklerde gözlenen klinik bulgular: anoreksiya %63 (7/11), uyuşukluk ve apati %63 (7/11), ateş %54 (6/11), soluk mukozal zarlar %54 (6/11), lenfadenomegali %18 (2/11), kusma %9 (1/11) ve ishal %9 (1/11). Kan sayımı, köpeklerin %70 (8/11)’inde anemi olduğunu gösterdi. Lökosit bozuklukları değişkendi; %27’sinde (3/11) lökositoz ve %46’sında (5/11) lökopeni vardı. 18S rRNA geninin sekanslanmış örnekleri çeşitli ülkelerden elde edilen B. canis vogeli sekansları ile %99 benzerlik göstermiştir

Babesia spp. in Dogs from Córdoba, Colombia

Canine babesiosis is a tick-borne disease with worldwide distribution, caused by intra-erythrocytic protozoa of the genus Babesia. The presentstudy describes the clinical features and molecular detection of B. canis vogeli in dogs in Córdoba, Colombia. Blood samples were taken fromdogs with clinical signs compatible with tick-borne diseases. Peripheral blood samples were examined microscopically for the presence ofBabesia spp. merozoites and trophozoites. Blood sample obtained by jugular or cephalic venipuncture were used for DNA extraction. PCRassay was carried out using primers for the 18S rRNA gene of Babesia spp. of the 42 dogs suspected of babesiosis, 23 were females and 19males. Peripheral blood examination demonstrated intraerythrocytic piroplasms compatible with Babesia spp. in 23 of 42 (54.7%) dogs. Themorphometric study showed that 73% (17/23) were large babesias (2.4x5 µm) and 26% (6/23) were small Babesia (1x3.2 µm). Eleven of 42(26%) blood samples were positive for B. canis vogeli. Clinical signs found in positive dogs were: anorexia 63% (7/11), lethargy and apathy 63%(7/11), fever 54% (6/11), pale mucous membranes 54% (6/11), lymphadenomegaly 18% (2/11), vomiting 9% (1/11) and diarrhea 9% (1/11).Blood counts showed that 70% (8/11) dogs had anemia. Leukocyte disorders were variable; 27% (3/11) had leukocytosis and 46% (5/11) hadleukopenia. Sequenced samples of the 18S rRNA gene showed 99% identity with B. canis vogeli sequences from several countries.

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