Muhammed Enes İNANÇ, Doğukan ÖZEN, Kemal Tuna OLGAC, Havva ALEMDAR, Koray TEKİN, Ongun UYSAL

Kolestrol ve 7-Dehidrokolestrolün Farklı Gliserol Oranları İle Boğa Sperması Dondurulmasına Etkisi Öz

Bu çalışmanın amacı, 7-dehidrokolesterol yüklü siklodekstrin (7-DHCLC) ve kolesterol yüklü siklodekstrin (CLC) ‘in farklı gliserol (G) oranları ilavesi ile boğa sperması dondurulması üzerindeki etkilerini değerlendirmektir. Üç adet boğaya ait sperma örnekleri toplandı, birleştirildi ve yedi gruba ayrıldı; biri kontrol (C) ile, diğer altı grup ise CLC veya 7-DHCLC (1.5 mg/120x106 ) ve farklı G oranlarını (%3, 6 ve 9) içeren standart Tris sulandırıcısı ile sulandırıldı. Sulandırılan spermalar 0.25 mL’lik payetlerde donduruldu. Çözdürme sonrası gruplar hareket karakteristikleri ve motilite, viabilite, akrozom bütünlüğü, mitokondriyal aktivasyon, fonksiyonel membran bütünlüğü ve anormal spermatozoa oranları yönünden değerlendirildi. En yüksek membran bütünlüğü, viabilite ve motilite %9 G ilavesi ile 7-DHCLC grubunda tespit edildi (P

Effect of Cholesterol and 7-Dehydrocholesterol on Bull Semen Freezing with Different Rates of Glycerol

The aim of this study was to evaluate the effect of 7-dehydrocholesterol loaded cyclodextrin (7-DHCLC) and cholesterol loaded cyclodextrin (CLC)with the addition of different glycerol (G) rates on bull semen cryopreservation. Semen samples of three bulls were pooled and divided into sevengroups; control group (C) and the other six were extended with standard Tris extender including different G rates (3, 6 and 9%) with CLC or 7-DHCLCto reach 1.5 mg/120x106. Extended samples were frozen in 0.25 mL straws. After thawing, motion characteristics and motility, viability, acrosomeintegrity, mitochondrial activation, functional membrane integrity and abnormal spermatozoa rates were assessed. The highest membrane integrity,viability and total motility were detected in 7-DHCLC with 9% of G group (P

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