Hong SHEN, Zhitao WANG, Xuhai WANG, Yongsheng ZHANG, Song JIANG, Xin LI, Bin JIA

MHC-DRB1/DQB1 Genes Polymorphism and Its Association with Resistance to Cystic Echinococcosis in Chinese Merino Sheep

Bu çalışmada tek sarmal konformasyon polimorfizm (SSCP) metodu kullanılarak DRB1 ve DQB1 genlerinin ekzon 2'leri ile Çin Merinos koyununda Kistik Echinococcosis (CE) polimorfizmleri arasındaki ilişki araştırıldı. Çalışmada 96 CE pozitif ve 115 negatif koyun kullanıldı. DRB1 geninde 22 allel tarafından kontrol edilen 53 genotip ile DQB1 geninde 17 allel tarafından kontrol edilen 47 genotip bulunduğu tespit edildi. CE pozitif ve negatif hayvanlarda allel frekansları karşılaştırıldığında DRB1 allel k (P

MHC-DRB1/DQB1 Gen Polimorfizmi ve Çin Merinos Koyununda Kistik Echinococcosise Dirençle İlişkisi

In this study, we used the single strand conformation polymorphism (SSCP) method to analyze the associations between polymorphisms of exon 2 of the DRB1 and DQB1 genes and Cystic Echinococcosis (CE) in Chinese Merino sheep. We examined 96 CE positive sheep and 115 negative sheep in this study. The results showed thatthere were 53 genotypes controlled by 22 alleles in the DRB1 gene and 47 genotypes controlled by 17 alleles in the DQB1 gene. The comparison of allele frequencies in the CE positive and negative animals revealed that DRB1 alleles k (P<0.05), q , t (P<0.01) and alleles a , l (P<0.01). These results indicate a strong association between these alleles k,q,t and CE resistance, the alleles a,l are associated with CE susceptibility. We analyzed the DRB1 genotype frequencies and found that the genotypes KK and TT (P<0.05) are associated with CE resistance, while AA, LL (P<0.01), and AL (P<0.05) were related to CE susceptibility. In DQB1 exon 2 the d and e alleles (P<0.01) were related to CE resistance, while c and k (P<0.01) were significantly related to CE susceptibility. We analyzed the DQB1 genotype frequencies and found that DD and EG (P<0.01) were associated with CE resistance, while genotypes CC, KK, and CK (P<0.01) were associated with CE susceptibility. Using haplotype analysis and artificially infection tests with Echinococcus granulosus (EG), we found that the DRB1-TT/DQB1-EE was a CE resistant haplotype in Chinese Merino sheep.

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