BEREN BAŞARAN KAHRAMAN, Kemal METİNER, BELGİ DİREN SIĞIRCI, BARAN ÇELİK, M. Cemal ADIGÜZEL, A. Funda BAĞCIGİL, Serkan İKİZ, N. Yakut ÖZGÜR, SEYYAL AK

The Effect of Different Storage Temperatures and Times on the Viability of Helicobacter pullorum [1]

Bu deneysel çalışmada, 4 adet civcive 1x109 cfu/ml H. pullorum, ATCC 51801 suşu oral yolla inokule edildi. Dışkı örnekleri toplandı ve dışkı havuzu oluşturuldu. Toplamda dışkı havuzundan alınan 77 svab -18°C, +4°C ve + 20-25°C'de saklandı ve 24 saat içinde her saat başı kültürel metot ile incelendi. Sonuç olarak, H. pullorum'un dayanıklılığı +25°C'de 11 saat, +4°C'de 14 saat ve -18°C'de 16 saat olarak belirlendi.

Helicobacter pullorum'un Değişik Isılarda ve Sürelerde Dayanıklılığının Belirlenmesi

In the experimental study, four chicks were inoculated orally with 1x10 cfu/ml of H. pullorum, ATCC 51801 strain. Stool samples from each animal were collected and a pool of faecal content has been achieved. Totally 77 swabs taken from the sample pool were stored at -18°C, +4°C, + 25°C, respectively and were examined by culture every hour within first 24 h. In conclusion, the survival time of H. pullorum was determined as 11 hours at +25°C, 14 hours at +4°C and 16 hours at -18°C.

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