ONUR BAŞBUĞ, Nevin TUZCU, ALPARSLAN COŞKUN, UĞUR AYDOĞDU, Akın YIĞIN, Zahid T. AĞAOĞLU

Tumour Necrosis Factor-alpha, Haptoglobin, Serum Amyloid A and Neopterin Levels in Cattle with Lumpy Skin Disease [1][2]

Lumpy Skin Disease (LSD), vücudun çeşitli bölgelerinde nodül oluşumu ile karekterize sığırların viral bir hastalığıdır. Bu çalışmada; LSD'li sığırlarda albümin, tümör nekroz faktör-? (TNF-?), haptoglobin (Hp), serum amyloid A (SAA) ve neopterinin, hastalığın klinik seyrini değerlendirme, inflamatuar sürecin gösterilmesi ve bu testlerin öneminin belirlenmesi amaçlanmıştır. Bu çalışma, doğal olarak LSD'li 20 hasta sığır ile aynı bölgeden 10 sağlıklı sığır olmak üzere toplam 30 hayvan üzerinde yürütüldü. LSD'li sığırların albümin konsantrasyonun önemli düzeyde (P=0.004) düşük olduğu; serum Hp (P

Lumpy Skin Disease'li Sığırlarda Tümör Nekroz Faktör-alfa,Haptoglobin, Serum Amiloid A ve Neopterin Düzeyleri

Lumpy skin disease (LSD) is a viral disease of cattle, characterised by the formation of nodules in different parts of the body. In this study, it was conducted to assess the pattern of changes of albumin, tumour necrosis factor-alpha (TNF-?), haptoglobin (Hp), serum amyloid A (SAA) and neopterin levels in cattle with LSD, to assess the clinical course of the disease, and to the demonstration of inflammatory process in cattle with LSD. This study was carried out in 30 cattle, including 20 animals naturally infected with LSD and 10 healthy animals. It was determined that, in the cattle infected with LSD, while albumin concentrations had significantly decreased (P=0.004) in comparison to the control group, Hp (P<0.001), SAA (P<0.001) and neopterin (P<0.001) concentrations had significantly increased. Receiver operating characteristic (ROC) curve was used to calculate the sensitivity and specificity of Hp, SAA and neopterin. The cut-off values of the healthy and infected cattle for Hp, SAA and neopterin were determined to be 0.196 mg/mL, 41.38 µg/mL and 23.93 nmol/mL, respectively. At these cut-off values, high levels of sensitivity (85% for Hp, 95% for SAA and 70% for neopterin) and specificity (90%) were detected. It was determined that SAA levels were of higher sensitivity and specificity compared to Hp and neopterin levels with respect to the demonstration of inflammation associated with LSD. Furthermore, the clinical picture of the disease was found to be significantly correlated with the Hp, SAA and neopterin levels.

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Basbug O, Gul Y: Investigations on hemolysis in Cows with tropical theileriosis. Kafkas Univ Vet Fak Derg, 17, 421-427, 2011. DOI: 10.9775/ kvfd.2010.3664
Beutler B, Cerami A: The biology of cachectin/TNF-? primary mediator of the host response. Annu Rev Immunol, 7, 625-655, 1989. DOI: 10.1146/ annurev.iy.07.040189.003205
Chan JP, Chang C, Hsu W, Liu W, Chen T: Association of increased serum acute-phase protein concentrations with reproductive performance in dairy cows with postpartum metritis. Vet Clin Pathol, 39, 72-78, 2010. DOI: 1111/j.1939-165X.2009.00182.x
Eckersall PD, Bell R: Acute phase proteins: Biomarkers of infection and inflammation in veterinary medicine. Vet J, 185, 23-27, 2010. DOI: 1016/j.tvjl.2010.04.009
Heegaard PMH, Godson DL, Toussaint MJM, Tionerhoi K, Larsen LE, Viuff B, Ronsholt L: The acute phase response of haptoglobin and serum amyloid A (SAA) in cattle undergoing experimental infection with bovine respiratory syncytial virus. Vet Immunol Immunopathol, 77, 151-159, 2000. DOI: 10.1016/S0165-2427(00)00226-9
Jawor P, Steiner S, Stefaniak T, Baumgartner W, Rzasa A: Determination of selected acute phase proteins during the treatment of limb diseases in dairy cows. Vet Med, 53, 173-183, 2008.
Orro T, Pohjanvirta T, Rikula U, Huovilainen A, Alasuutari S, Sihvonen L, Pelkonen S, Soveri T: Acute phase protein changes in calves during an outbreak of respiratory disease caused by bovine respiratory syncytial virus. Comp Immunol Microbiol Infect Dis, 34, 3-29, 2011. DOI: 1016/j.cimid.2009.10.005
Plata-Nazar K, Luczak G, Gora-Gebka M, Liberek A, Kaminska B: Serum neopterin concentration in children with viral gastroenteritis. Pteridines, 21, 11-16, 2010. DOI: 10.1515/pteridines.2010.21.1.11
Prat C, Dominguez J, Andreo F, Blanco S, Pallares A, Cuchillo F, Ramil C, Ruiz-Manzano J, Ausina V: Procalcitonin and neopterin correlation with aetiology and severity of pneumonia. J Infect, 52, 169- , 2006. DOI: 10.1016/j.jinf.2005.05.019
Gruys E, Toussaint MJM, Niewold TA, Koopmans SJ: Acute phase reaction and acute phase proteins. J Zhejiang Univ Sci, 11, 1045-1056, 2005.
Guzelbektas H, SenI, Ok M, ConstablePD, Boydak M, Coskun A: Serum amyloid A and haptoglobin concentrations and liver fat percentage in lactating dairy cows with abomasal displacement. J Vet Intern Med, 24, 213-219, 2010. DOI: 10.1111/j.1939-1676.2009.0444.x
Horadagoda NU, Knox KMG, Gibbs HA, Reid SWJ, HoradagodaA, Edwards SER, Eckersall PD: Acute phase proteins in cattle: Discrimination between acute and chronic inflammation. Vet Rec, 144, 437-441, 1999. DOI: 10.1136/vr.144.16.437
Nazifi S, Ansari-Lari M, Ghafari N, Mohtarami S, Ghezelbash A, Tabandeh MR: Evaluation of sialic acids, TNF-?, INF-?, and acute-phase proteins in cattle infected with foot-and-mouth disease. Comp Clin Pathol, , 23-28, 2012. DOI: 10.1007/s00580-010-1059-5
Berdowska A, Zwirska-Korczala K: Neopterin measurement in clinical diagnosis. J Clin Pharm Ther, 319-329, 2001. DOI: 10.1046/j.1365- 2001.00358.x
Hoffmann G, Wirleitner B, Fuchs D: Potential role of immune system activation-associated production of neopterin derivatives in humans. Inflamm Res, 52, 313-321. 2003. DOI: 10.1007/s00011-003-1181-9
Eisenhut M: Neopterin in diagnosis and monitoring of infectious diseases. J Biomark, 1, 1-10. 2013. DOI: 10.1155/2013/196432
Kaleli I, Demir M, Cevahir N, Yılmaz M, Demir S: Serum neopterin levels in patients with replicative and nonreplicative HBV carriers. BMC Infect Dis, 6, 157, 2006. DOI: 10.1186/1471-2334-6-157
Abutarbush SM, Ababneh MM, Al Zoubi IG, Al Sheyab OM, Al Zoubi MG, Alekish MO, Al Gharabat RJ: Lumpy skin disease in Jordan: Disease emergence, clinical signs, complications and preliminary- associated economic losses. Transbound Emerg Dis, 62, 549-554, 2015. DOI: 10.1111/tbed.12177
Coetzer JAW: Lumpy skin disease. In, Coetzer JAW, Tustin RC (Eds): Infectious Diseases of Livestock. 2nd ed., 1268-1276, University Press
Southern Africa, Oxford, 2004.
Gurcay M, Sait A, Parmaksız A, Kilic A: The detection of Lumpy Skin Disease virus infection by clinical findings and PCR method in Turkey. Kafkas Univ Vet Fak Derg, 21, 417-420, 2015. DOI: 10.9775/ kvfd.2014.12364
Kitching PR, Mellor PS: Insect transmission of Capripox viruses. Res Vet Sci, 40, 255-258, 1986.
El-Neweshy MS, El-Shemey TM, Youssef SA: Pathologic and immunohistochemical findings of natural lumpy skin disease in Egyptian cattle. Pak Vet J, 33, 60-64, 2012.
Magori-Cohen R, Louzoun Y, Herziger Y, Oron E, Arazi A, Tuppurainen E, Shpigel NY, Klement E: Mathematical modelling and evaluation of the different routes of transmission of lumpy skin disease virus. Vet Res, 43, 1, 2013. DOI: 10.1186/1297-9716-43-1
Salib FA, Osman AH: Incidence of lumpy skin disease among Egyptian cattle in Giza Governorate. Egypt Vet World, 4, 162-167, 2011.
El-Kenawy AA, El-Tholoth MS: Lumpy skin disease virus identification in different tissues of naturally infected cattle and chorioallantoic membrane of embryonated chicken eggs using immunofluorescence, immunoperoxidase techniques and polymerase chain reaction. Int J Virol, , 158-166. 2011. DOI: 10.3923/ijv.2011.158.166
Sharawi SS, Abd ERI: The utility of polymerase chain reaction for diagnosis of lumpy skin disease in cattle and water buffaloes in Egypt. Rev Sci Tech Off Int Epizoot, 30, 821-830, 2011.
Stubbs S, Oura CAL, Henstocka M, Bowden TR, King DP, Tuppurainen ES: Validation of a high-throughput real-time polymerase chain reaction assay for the detection of capripoxviral DNA. J Virol Methods, 179, 419-422, 2012. DOI: 10.1016/j.jviromet.2011.11.015
Nielsen BH, Jacobsen S, Andersen PH, Niewold TA, Heegaard PM: Acute phase protein concentrations in serum and milk from healthy cows, cows with clinical mastitis and cows with extramammary inflammatory conditions. Vet Rec, 154, 361-365, 2004. DOI: 10.1136/vr.154.12.361
Murata H, Shimada N, Yoshioka M: Current research on acute phase proteins in veterinary diagnosis: An overview. Vet J, 168, 28-40, 2004. DOI: 1016/S1090-0233(03)00119-9
Höfner MC, Fosbery MW, Eckersall PD, Donaldson AL: Haptoglobin response of cattle infected with foot and mouth disease virus. Res Vet Sci, , 125-128, 1994. DOI: 10.1016/0034-5288(94)90093-0
Fuchs D, Hausen A, Reibnegger G, Werner ER, Dietrych MP, Wachter H: Neopterin as a marker for activated cell-mediated immunity: Application in HIV infection. Immunol Today, 9, 150-155, 1998. DOI: 1016/0167-5699(88)91203-0
Watcher H, Fuchs D, Hausen A, Reibnegger G, Werner ER: Neopterin as a marker for activation of cellular immunity: Immunologic basis and clinical application. Adv Clin Chem, 27, 81-141, 1989. DOI: 10.1016/S0065- (08)60182-1
Ercan N, Tuzcu N, Basbug O, Kurtuluş G, Isidan H, Ograk YZ: The evaluation of important biomarkers in healthy cattle. Kafkas Univ Vet Fak Derg, 20, 749-755, 2014. DOI: 10.9775/kvfd.2014.11066
Francisco NM, Hsu NJ, Keeton R, Randell P, Sebesho B, Allie N, Govender D, Quesniaux V, Ryffel B, Kellaway L, Jacobs M: TNF- dependent regulation and activation of innate immune cells are essential for host protection against cerebral tuberculosis. J Neuroinflamm, 12, 14, 2015. DOI: 10.1186/s12974-015-0345-1
Sordillo LM, Peel JE: Effect of interferon on the production of tumor necrosis factor during acute Escherichia coli mastitis. J Dairy Sci, 75, 2119- , 1992. DOI: 10.3168/jds.S0022-0302(92)77971-5
Rodostits OM, Gay CC, Hinhcliff KW, Constable PD: Appendix 2 Reference Laboratory Values. In, Veterinary Medicine - A Textbook of the Diseases of Cattle, Sheep, Goats, Pigs and Horses. 10th ed., 2047-2050, WB Saunders, London,2006.
Wi YM, Kim JM, Peck KR: Serum albumin level as a predictor of intensive respiratory or vasopressor support in influenza A (H1N1) virus infection. Int J Clin Pract, 68, 222-229, 2014. DOI: 10.1111/ijcp.12249