MEHMET ÇİFTÇİ, BESTAMİ DALKILIÇ, Ülkü Gülcihan ŞİMŞEK, MEHMET ALİ AZMAN, ZEKİ ERİŞİR, MEHTAP ÖZÇELİK, ÖKKEŞ YILMAZ, SEDA İFLAZOĞLU MUTLU, Fatma TERLEMEZ, MUAMMER BAHŞİ

Effects of Dietary Soapwort Extract Supplementation on Laying Performance, Blood Biochemical Parameters, Fatty Acid Profile of Breast Meat and Antioxidative Potential of Liver and Heart Tissues in Cold Stressed Laying Japanese Quail

Bu çalışmada, karma yeme ilave edilen çöven ekstraktının (SE), kronik soğuk stresine maruz bırakılan Japon bıldırcınlarında performans, yumurta kalitesi, bazı organ ağırlıkları, bazı serum parametreleri, karaciğer ve kalp dokularının antioksidan durumları ve göğüs eti yağ asidi profili üzerine etkileri araştırılmıştır. Toplam olarak 63 adet 45 günlük bıldırcın, 3 tekerrürlü 3 gruba ayrılmıştır. Gruplardan biri mısır-soya küspesine dayalı temel yem ile (kontrol grubu), diğer gruplar temel yeme 50 ve 100 ppm SE ilave edilen yemle beslenmişlerdir. Soğuk stresi her akşam 22.00-06.00 saatleri arasında kümes ısısı 7±1°C'ye düşürülerek uygulanmıştır. SE ilavesi performans, bazı organ ağırlıkları ile yumurta ve albümün ağırlığı hariç yumurta kalite parametrelerini etkilememiştir. Serum glikoz, trigliserit, ürik asit ve AST düzeyleri SE-50 grubunda düşmüştür. 100 ppm SE ilavesi karaciğer ve kalp dokularında MDA düzeylerini düşürürken, karaciğer GSH-Px enzim aktivitesi her iki SE grubunda artmıştır. 100 ppm SE ilavesi göğüs etinde oleik asit ve MUFA düzeylerini düşürürken, linoleik, linolenik ve araşidonik asit, PUFA, toplam omega 6 ile omega 6/omega 3 düzeylerini önemli ölçüde yükseltmiştir. Sonuç olarak; soğuğa maruz kalan yumurtacı bıldırcınlarda diyete ilave edilen SE'nin performans parametrelerini etkilemeden oksidatif stresin zararlı etkilerini azaltabileceği ortaya koyulmuştur.

Soğuk Stresine Maruz Bırakılan Japon Bıldırcınlarında Diyete İlave Edilen Çöven Ekstraktının Yumurtlama Performansı, Kan Biyokimyasal Parametreleri, Göğüs Eti Yağ Asidi Profili İle Karaciğer ve Kalp Dokusu Antioksidatif Potansiyeli Üzerine Etkisi

The purpose of this study was to evaluate the effects of dietary Soapwort Extract (SE) supplementation on laying performance, egg quality, some offal weights, some serum parameters, antioxidant status of liver and heart tissues and fatty acid composition of breast meat in chronic cold stressed Japanese quails. A total of sixty three 45-day-old Japanese quails were divided into three groups with three replicate. One group was fed with the corn-soybean based diet alone (control group) and the others were fed with the basal diet supplemented by 50 and 100 ppm SE. Cold stress was applied every night between 22.00 to 06.00 h as 7±1°C. SE supplementation had no significant effect on laying performance, offal weights and egg quality parameters with the exception of egg and albumen weights. Serum glucose, triglyceride, uric acid and aspartate aminotransferase (AST) levels were reduced in SE-50 group. While supplementation of 100 ppm SE decreased both liver and heart MDA levels, both amounts of SE increased the liver GSH-Px enzyme activity. Supplementation of 100 ppm SE decreased the levels of oleic acid and MUFA in breast meat, and it significantly increased the linoleic, linolenic and arachidonic acid, PUFA, total omega 6 and the ratio of omega 6/omega 3. It is displayed that SE supplementation to diets of laying quails exposed to cold stress can alleviate the detrimental effects of oxidative stress with no affecting on performance parameters.

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Sahin N, Sahin K, Onderci M: Vitamin E and selenium supplementation to alleviate cold-stress-associated deterioration in egg quality and egg yolk mineral concentrations of Japanese quails. Biol Trace Elem Res, 96, 189, 2003. DOI: 10.1385/BTER:96:1-3:179
Kovacs P, Juranek I, Stankovicova T, Svec P: Lipid peroxidation during acute stress. Pharmazie, 51, 51-53 1996.
Wallace RJ, Oleszek W, Franz C, Hahn I, Baser KH, Mathe A, Teichmann K: Dietary plant bioactives for poultry health and productivity. Br Poult Sci, 51, 461-487, 2010. DOI: 10.1080/00071668.2010.506908
Moghimipour E, Handali S: Saponin: Properties, methods of evaluation and applications. Ann Res Rev Biol, 5, 207-220, 2015. DOI: 9734/ARRB/2015/11674
Fleck JD, Kauffmann C, Spilki F, Lencina CL, Roehe PM, Gosmann G: Adjuvant activity of Quillaja brasiliensis saponins on the immune responses to bovine herpesvirus type 1 in mice. Vaccine, 24, 7129-7134, DOI: 10.1016/j.vaccine.2006.06.059
Xiao X, Bai P, Bui Nguyen TM, Xiao J, Liu S, Yang G, Hu L, Chen X, Zhang X, Liu J, Wang H: The antitumoral effect of Paris saponin I associated with the induction of apoptosis through the mitochondrial pathway. Mol Cancer Ther, 8, 1179-1188, 2009. DOI: 10.1158/1535-7163. MCT-08-0939
Sidhu GS, Oakenfull DG: A mechanism for the hypocholesterolaemic activity of saponins. Br J Nutr, 55, 643-649, 1986. DOI: 10.1079/ BJN19860070
Coleman JJ, Okoli I, Tegos GP, Holson EB, Wagner FF, Hamblin MR, Mylonakis E: Characterization of plant-derived saponin natural products against Candida albicans. ACS Chem Biol, 5, 321-332, 2010. DOI: 10.1021/ cb900243b
Arslan I, Celik A: Saponin rich fractions (SRPs) from soapwort show antioxidant and hemolytic activity. APCBEE Procedia, 7, 103-108, 2013. DOI: 10.1016/j.apcbee.2013.08.019
Sur P, Chaudhuri T, Vedasiromoni JR, Gomes A, Ganguly DK: Antiinflammatory and antioxidant property of saponins of tea (Camellia sinensis (L) O. Kuntze) root extract. Phytother Res, 15, 174-176, 2001. DOI: 1002/ptr.696
Rowland LO, Pleyler JE, Bradley JW: Yucca schidigera extract effect on egg production and house ammonia levels. Poult Sci, 55, 2086-2093,
Baytop T: Türkiye'de bitkiler ile tedavi, geçmişte ve bugün. İstanbul Üniversitesi Yayınları, No: 3255, Eczacılık Fakültesi Yayınları, No: 40, 520s. İstanbul, 1984.
Özçelik H, YıldırımB: Türkiye çövenlerinin (Gypsophila L. ve Ankyropetalum Fenzl spp.) ekonomik önemi, kullanım olanakları ve korunması üzerine düşünceler. SDU Fac Forestry J, 12, 57-61, 2011.
Cheeke PR: Actual and potential applications of Yucca schidigera and Quillaja saponaria saponins in human and animal nutrition. J Anim Sci, 77, 10, 2000. DOI: 10.1007/978-94-015-9339-7_25
AOAC: Official Methods of Analysis Association of AOAC International. th ed., AOAC International Maryland, 2000.
Crampton EW, Maynard LA: The relation of cellulose and lignin content to nutritive value of animal feeds. J Nutr, 15, 383-395, 1983.
Lane JH, Eynon L: Determination of reducing sugars by means of Fehling's solution with methylene blue as internal indicator. J Soc Chem Ind Trans, 42, 32-36, 1923.
Carpenter KJ, Clegg KM: The metabolizable energy of poultry feding stuffs in relation to their chemical composition. J Sci Food Agric, 7, 45-51, DOI: 10.1002/jsfa.2740070109
Lalitha T, Seshadri R, Venkataraman LV: Isolation and properties of saponins from Madhuca butyracea seeds. J Agric Food Chem, 35, 744-748, DOI: 10.1021/jf00077a024
National Research Council: Nutrient Requirements of Poultry, 9th ed., National Academic Press, Washington, DC, 1994.
Anonymous: Poultry carcass-rules for carcass dissecting. Institute of Turkish Standards, TS 5890, Ankara, Turkey, ICS 67.120.10; 67.120.20,
Placer AZ, Linda LC, Johnson B: Estimation of product lipid peroxidation (malonyldialdehyde) in biochemical systems. Anal Biochem, , 359-364, 1966. DOI: 10.1016/0003-2697(66)90167-9
Sun Y, Oberley LW, Li Y: A simple method for clinical assay of superoxide dismutase. Clin Chem, 34, 497-500, 1988.
Lawrence RA, Burk RF: Glutathione peroxidase activity in selenium- deficient rat liver. Bioch Bioph Res Comm, 71, 952-958, 1976. DOI: 1016/0006-291X(76)90747-6
Sedlak J, Lindsay RH: Estimation of total protein bound and non- protein sulfhydryl groups in tissue with Ellman reagent. Anal Biochem, 25, 205, 1968. DOI: 10.1016/0003-2697(68)90092-4
Hara AR, Radin NS: Lipid extraction of tissues with a low- toxicity solvent. Anal Biochem, 90, 420-426, 1978. DOI: 10.1016/0003- (78)90046-5
Christie WW: Gas chromatography and lipids. The Oil Press, Glaskow, , 1992.
SPSS Inc: SPSS for Windows Release 11.5 (6 Sep. 2002), Standard
Version, Copyright SPSS Inc, Chicago, 2002.
Hostettmann K, Marston A: Saponins. Cambridge: Cambridge University Press, UK, 1995.
Kutlu HR, Gorgulu M, Unsal I: Effects of dietary Yucca schidigera powder on performance and egg cholesterol content of laying hens. J Appl Anim Res, 20, 49-56, 2001. DOI: 10.1080/09712119.2001.9706736
Aslan R, Dundar Y, Eryavuz A, Bulbul A, Kucukkurt I, Fidan AF, Akinci Z: Effects of different dietary levels of Yucca shidigera powder (Deodorase) added to diets on performance, some hemotological and biochemical blood parameters and total antioxidant capacity of laying hens. Rev Med Vet, 156, 350-355, 2005.
Heywang BW, Bird HR: The effect of alfalfa saponin on the growth, diet consumption, and efficiency of diet utilization of chicks. Poult Sci, 33, 241, 1954. DOI: 10.3382/ps.0330239
Anderson JO: Effect of alfalfa saponin on the performance of chicks and laying hens. Poult Sci, 36, 873-876, 1957. DOI: 10.3382/ps.0360873
Heywang BW, Thompson CR, Kemmerer AR: Effect of alfalfa saponin on laying chickens. Poult Sci, 38, 968-971, 1959. DOI: 10.3382/ps.0380968
Jenkins KJ, Atwal AS: Effects of dietary saponins on fecal bile acids and neutral sterols, and availability of vitamins A and E in the chick. J Nutr Biochem, 5, 134-135, 1994. DOI: 10.1016/0955-2863(94)90084-1
Whitehead CC, Mcnab JM, Griffin HD: The effects of low dietary concentrations of saponin on liver lipid accumulation and performance in laying hens. Br Poult Sci, 22, 281-288, 1981. DOI: 1080/00071688108447887
Cheeke PR, Powley JS, Nakaue HS, Arscott GH: Feed preference responses of several avian species fed alfalfa meal, high-and low-saponin alfalfa, and quinine sulfate. Can J Anim Sci, 63, 707-710, 1983. DOI: 4141/cjas83-080
Southon S, Johnson IT, Gee JM, Price KR: The effect of Gypsophila saponins in the diet on minerals status and plasma cholesterol concentration in the rat. Br J Nutr, 59, 49-55, 1988. DOI: 10.1079/ BJN19880008
Southon S, Wright AJA, Price KR, Fairweather-Tait SJ, Fenwick GR: The effect of three types of saponin on iron and zinc absorption from a single meal in the rat. Br J Nutr, 59, 389-396, 1988. DOI: 10.1079/ BJN19880048
Afrose S, Hossain MS, Maki T, Tsujii H: Hypocholesterolemic response to Karaya saponin and Rhodobacter capsulatus in broiler chickens. Asian- Aust J Anim Sci, 23, 733-741, 2010. DOI: 10.5713/ajas.2010.90481
Yang CJ, Yang IY, Oh DH, Bae IH, Cho SG, Kong IG, Uuganbayar D, Nou IS, Choi KS: Effect of green tea by-product on performance and body composition in broiler chicks. Asian-Aust J Anim Sci, 16, 867-872, DOI: 10.5713/ajas.2003.867
Kucukkurt I, Dundar Y: Effects of dietary Yucca schidigera supplementation on plasma leptin, insulin, iodated thyroid hormones and some biochemical parameters in rats. Rev Med Vet, 7, 362-367, Johnson JS: Heat stress alters animal physiology and post-absorptive metabolism during pre- and postnatal development. Graduate Theses and Dissertations, Paper: 13982, 2014.
Virden WS, Kidd MT: Physiological stress in broilers: Ramifications on nutrient digestibility and responses. J Appl Poult Res, 18, 338-347, 2009. DOI: 10.3382/japr.2007-00093
Dabbert CB, Lochmiller RL, Teeter RG: Thermal stress influences clinical chemistry values of northern bobwhite (Colinus virginianus). Comp Haematol Int, 6, 120-122, 1996. DOI: 10.1007/BF00426054
Kucukkurt I, Ince S, Enginar H, Eryavuz A, Fidan AF, Kargioglu M: Protective effects of Agrostemma githago L. and Saponaria officinalis L. extracts against ionizing radiation-induced oxidative damage in rats. Rev Med Vet, 162, 289-296, 2011.
Rohaida AR, Alimon AR, Sazili AQ: Fatty acid composition of breast and thigh muscles of broilers fed diets supplemented with candle nut kernel meal subjected to different heat treatments. Mal J Anim Sci, 17, 47- , 2014.
Brogna DM, Nasri S, Salem HB, Mele M, Serra A, Bella M, Priolo A, Makkar HP, Vasta V: Effect of dietary saponins from Quillaja saponaria L. on fatty acid composition and cholesterol content in muscle Longissimus dorsi of lambs. Animal, 5, 1124-1130, 2011. DOI: 10.1017/ S1751731111000048