BUKET BAKIR, Sevda YILDIZ ELİŞ, EBRU KARADAĞ SARI, HASAN ASKER, Mümtaz NAZLI

Immunohistochemical Distribituon of COX-1, COX-2, and TGF?-1 in the Duodenum of Rats Treated with Capsaicin

Bu çalışmada capsaicin uygulanan ratların duodenum dokusunda siklooksijenaz-1 (COX-1), siklooksijenaz-2 (COX-2) ve transforme edici büyüme faktörü ?-1 (TGF?-1)'in dağılımını araştırmak amaçlandı. Ratlar iki gruba ayrıldı (her grupta n=10). Capsaicin uygulanan deneme grubuna (1 mg/kg, subkutan yolla) %10 ethanol, %1 tween, %80 distile su karışımı bir haftalık periyot boyunca hergün enjekte edildi. COX-1, COX-2 ve TGF?-1'in dağılımı villus ve kript epitel hücreleri ve düz kas hücrelerinde tespit edildi. Sonuçta, bu çalışmada capsaicin uygulamasının COX-1 ve COX-2 ve TGF?-1 salınımını arttırdığı tespit edildi.

Capsaicin Uygulanan Sıçanların Duodenumda COX-1, COX-2 ve TGF?-1'in İmmünohistokimyasal Dağılımı

The purpose of this study was to investigate the effects of capsaicin on the duodenal distribution of cyclooxygenase-1 (COX-1), cyclooxygenase-2 (COX-2) and Transforming growth factor ?-1 (TGF?-1) in rats. Rats were divided into two groups (n =10 in each group). Experimental group treated with capsaicin (1 mg/kg, subcutaneously) was injected in 10% ethanol, 1% Tween and 80% distilled water everyday for a period of one week and in not injected control group. Localisations of COX-1, COX-2 and TGF?-1 were observed in villus and crypt ephitelial cells, and smooth muscle cells. In conclusion, in this study, capsaicin administration was found to increase the release of COX-1 and COX-2, and TGF?-1.

PDF

___

Lembeck F: Columbus, capsicum and capsaicin: Past, present and future. Acta Physiol Hung, 69, 265-273, 1987.
Szalasi A, Blumberg PM: Vanilliod (capsaicin) receptors and mechanisms. Pharmacol Rev, 51, 159-211, 1999.
Başak S, Dikicioglu E, Turgutanıt S, Sarıerler M: Early and late effects of capsaicin pretreatment in otitis media with effusion. Otol Neurotol, , 344-350, 2005.
Caterina MJ, Leffler A, Malmberg AB, Martin WJ, Trafton J, Petersen Zeitz KR, Kdtzenburg M, Basbaum AI, Julius D: Impaired nociception and pain sensation in mice lacking the capsaicin receptor. Science, 288, 313, 2000. DOI: 10.1126/science.288.5464.306
Erdost H, Ozfiliz N, Ozgüden OS, Ilhan T, Ozer A: Expression of a capsaicin reseptor (VRI) in the testis of mice after an application of capsaicin. Bull Vet Inst Pulaway, 51, 649-653, 2007.
Bakır B, Sarı EK: Immunohistochemical distribution of platelet derived growth factor-c and platelet derived growth factor receptor-alpha in small intestine of rats treated with capsaicin. Turk J Vet Anim Sci, 39, 160- , 2015. DOI: 10.3906/vet-1405-24
Nur G, Nazlı M, Yıldız SE: Immunohistochemical localization of transforming growth factor beta 2 and gene expression using real-time PCR in capsaicin-administered rat testis during puberty. Turk J Vet Anim Sci, 38, 377-382, 2014. DOI: 10.3906/vet-1302-16
Yıldız SE, Nazlı M, Nur G: Immunohistochemical distribution and gene expression of transforming growth factor alpha in ovarian tissue of rats treated with capsaicin in puberty. Turk J Med Sci, 43, 326-332, 2013. DOI: 10.3906/sag-1205-120
Smith WL, Garavito RM, Dewitt DL: Prostaglandin endoperoxide H synthases (Cyclooxygenases) -1 and -2. J Biol Chem, 271, 331-357, 1996. DOI: 10.1074/jbc.271.52.33157
Vane JR, Bakhlel YS, Botting RM: Cyclooxygenases-1 and -2. Annu Rev Pharmacol Toxicol, 38, 97-120, 1998. DOI: 10.1146/annurev. pharmtox.38.1.97
Williams CS, Dubois RN: Prostaglandin endoperoxide synthase: Why two isoforms? Am J Physiol, 270, 393-400, 1996.
Singer II, Kawka DW, Schloemann S: Cyclooxygenase-2 is induced in colonic epithelial cells in inflammatory bowel disease. Gastroenterology, , 297-306, 1998. DOI: 10.1016/S0016-5085(98)70196-9
Warner T, Mitchell JA: Cyclooxygenases: New forms, new inhibitors, and lessons from the clinic. FASEB J, 18, 790-804, 2004.
Wang F, Shi R, Zhao L: Expression and significance of TGF-?1 and VEGF in formation of new blood vessels after rabbit corneal suture. Recent Adv Ophthalmol, 28, 96-99, 2008.
Ohtomo K, Ebihara N, Matsuda A, Tokura T, Funaki T, Murakami A: Role of TGF-?1 in tissue eosinophilia associated with vernal kerato- conjunctivitis. Experimental Eye Res, 91, 748-754, 2010. DOI: 10.1016/j. exer.2010.08.025
Powell DW, Mifflin RC, Valentich JD, Crowe SE, Saade JI, West AB: Myofibroblasts, paracrine cells important in health and disease. Am J Physiol, 277, C1-C19, 1999.
Barnard JA, Beauchamp RD, Coffey RJ, Moses HL: Regulation of intestinal epithelial cell growth by transforming growth factor type ?. Proc Natl Acad Sci, 86, 1578-1582, 1989.
Barnard JA, Warwick GJ, Gold LI: Localization of transforming growth factor ? isoforms in the normal murine small intestine and colon. Gastroenterology, 105, 67-73, 1993.
Harada N, Okajima, Arai M, Kurihara H, Nagata N: Administration of capsaicin and isoflavone promotes hair growth by increasing insulin-l ike growth factor-I production in mice and in humans with alopecia. Growth horm IGF res, 17, 408-415, 2007.
Habib A, Bernard M, Lebret C, Creminon B, Esposito A, Tedgui J, Maclouf J: Regulation of the expression of cyclooxygenase-2 by nitric oxide in rat peritoneal macrophages. J Immunol, 158, 38-45, 1997.
Hoffman C: COX-2 in brain and spinal cord-implications for therepeutic use. Curr Med Chem, 7, 1113-1120, 2000.
Moran C, Morales L, Razo RS, Apolonio J, Quiroz U, Chavira R, Dominguez R: Effects of sensorial denervation induced by capsaicin injection at birth or on day three of life, on puberty, induced ovulation and pregnancy. Life Sci, 73, 2113-2125, 2003. DOI: 10.1016/S0024- (03)00598-8
Tütüncü S, Özfiliz N: Distribution of the vanilloid (capsaicin) receptor type 1 in the capsaicin treated rat ovaries on different sexual development periods. Rev Méd Vét, 162, 460-467, 2011.
Eccleston C: Role of psychology in pain management. Br J Anaesth, , 144-152, 2001. DOI: 10.1093/bja/87.1.144
Yoshimura M, Yonehara N, Ito T, Kawai Y, Tamura T: Effects of topically applied capsaicin cream on neurogenic imflammation and thermal sensitivity in rats. Jpn J Pharmacol, 82, 116-121, 2000.
Shure D, Senior RM, Griffin GL, Deuel TF: PDGF-AA homodimers are potent chemoattractants for fibro blast and neutrophils, and for monocytes activated by lymphocytes or cytokines. Biochem Biophys Res Commun, 186, 1510-1514, 1994. DOI: 10.1016/S0006-291X(05)81577-3
Dannenberg AJ, Altorki NK, Boyle JO, Dang C, Howe LR, Weskler BB, Subbaramaıah K: Cyclooxygenase-2: A pharmacological target for the prevention of cancer. Lancet Oncol, 2, 544-551, 2001. DOI: 10.1016/ S1470-2045(01)00488-0
Van Rees BP, Rıstımaki A: Cyclooxygenase-2 in carcinogenesis of the gastrointestinal tract. Scand J Gastroenterol, 36, 897-903, 2001.
Flower RJ: Drugs which inhibit prostaglandin biosythesis. Pharmacol Rev, 26, 33-67, 1974.
Sinatra R: Role of COX-2 inhibitors in the evolution of acute pain management. J Pain Symptom Manage, 24, S18-S27, 2002. DOI: 10.1016/ S0885-3924(02)00410-4
Yermakowa A, O'banion MK: Cyclooxygenases in the central nervous system: Implications for treatment of neurological disorders. Curr Pharm Des, 6, 1755-1776, 2000. DOI: 10.2174/1381612003398672
Harris RC, Beyer MD: Physiological regulation of cyclooxygenase- in the kidney. Am J Physiol Renal Physiol, 281, 1-11, 2001.
Sirois J, Sayasith K, Brown KA, Stock AE, Bouchard N, Dore M: Cyclooxygenase-2 and its role in ovulation: A 2004 account. Human
Reprod Update, 17, 373-385, 2006. DOI: 10.1093/humupd/dmh032
Asano K, Lilly CM, Drazen JM: Prostaglandin G/H synthase-2 is the constitutive and dominant isoform in cultured human lung epithelial cells. Am J Physiol, 271, L126-L131, 1996.
Fornai M, Blandizzi C, Colucco R: Role of cyclooxygenase-1 and -2 in the modulation of neuromuscular functions in the distal colon of humans and mice. Gut, 54, 608-616, 2005.
O'neil GP, Ford-Hudchinson AW: Expression of mRNA for cyclo- oxygenase-1 and cyclooxygenase-2 in human tissues. FEBS Lett, 330, 160, 1993. DOI: 10.1016/0014-5793(93)80263-T
Robertson RP: Dominance of cyclooxygenase-2 in the regulation of pancreatic islet prostaglandin synthesis. Diabetes, 47, 1379-1383, 1998. DOI: 10.2337/diabetes.47.9.1379
Sano H, Kawahito Y, Wilder RL: Expression of cyclooxygenase-1 and 2 in human colorectal cancer. Cancer Res, 55, 3785-3789, 1995.
Yasojima K, Schwab C, Mcgeer EG, Mcgeer PL: Distribution of cyclooxygenase-1 and cyclooxygenase-2 mRNAs and proteins in human brain and peripheral organs. Brain Res, 830, 226-236, 1998. DOI: 10.1016/ S0006-8993(99)01389-X
Zidar N, Odar K, Glavak D, Jer'se M, Zupanc T, Stajer D: Cyclo- oxygenase in normal human tissues - is COX-1 really a constitutive isoform, and COX-2 an inducible isoform? J Cell Mol Med, 13, 3753-3763, DOI: 10.1111/j.1582-4934.2008.00430.x
Zimmerman KC, Sarbia M, Schror K, Weber AA: Constitutive cyclooxygenase-2 expression in healthy human and rabbit gastric mucosa. Mol Pharmacol, 54, 536-534, 1998. DOI: 10.1124/mol.54.3.536
Miller DA, Lee A, Matsui Y, Chen EY, Moses HL, Derynck R: Complementary DNA cloning of the murine transforming growth factor-?3 (TGF-?3) precursor and the comparative expression of TGF-?3 and TGF-?1 messenger RNA in murine embryos and adult tissues. Mol Endocrinol, 3, 1926-1934, 1989. DOI: 10.1210/mend-3-12-1926
Fujiawara T, Stolker JM, Watanabe T, Rashid A, Longo P, Eshleman JR, Booker S, Lynch HT, Jass JR, Green JS, Kim H, Jen J, Vogelstein B, Hamilton SR: Accumulated clonal genetic alterations in familial and sporadic colorectal carcinomas with widespread instability in microsatellite sequences. Am J Pathol, 153, 1063-1078, 1998. DOI: 1016/S0002-9440(10)65651-9
Markowitzs D, Roberts B: Tumor suppressor activity of the TGF-beta pathway in human cancers. Cytokine Growth Factor Rev, 7, 93-102, 1997. DOI: 10.1016/1359-6101(96)00001-9
Koyama S, Podolsky DK: Differential expression of transforming growth factors ? and ? in rat intestinal epithelial cells. J Clin Invest, 83, 1773, 1989. DOI: 10.1172/JCI114080