İSMAİL AYTEKİN, FEYYAZ KAYA, HASAN ATALAY

Evaluation of Serum Haptoglobin, Ceruloplasmin and Pseudocholinesterase Levels in Cows with Botulism

Bu çalışmada botulismuslu ve sağlıklı ineklerde serum pseudokolinesteraz, seruloplazmin, haptoglobin, romatoid faktör, troponin I, C-reactive protein, üre, kreatin, kreatin kinaz (CK), alanine aminotransferase (ALT) ve aspartate aminotransferase (AST), fosfor ve kalsiyum seviyeleri araştırıldı. Çalışma ve kontrol grubunu gebe ve kuru dönemde iki ile beş yaşları arasında değişen 15 adet botulismuslu ve 10 adet sağlıklı holştayn inek oluşturdu. Parametrelerin serum düzeyleri Nefelometre BNII ve otoanalizör kullanılarak ölçüldü. Botilusmuslu hayvanların kan serumlarından ve silajdan fare inokulasyon testi yapılarak clostridium botulinum tip C ve tip D toksini tespit edildi. Serum troponin I, C-reactive protein, romatoid faktör, kreatin, kreatin kinaz, AST, fosfor ve kalsiyum seviyeleri botulismuslu ve sağlıklı ineklerde önemli bir değişiklik göstermedi. Botulismuslu ineklerde haptoglobin, seruloplazmin ve üre yüksek çıkarken, ALT ve pseudokolinesterazın sağlıklı ineklere göre daha düşük çıktığı tespit edildi. Botulismuslu ineklerde serum üre, ALT, haptoglobin, seruloplazmin ve özellikle pseudokolinesteraz düzeylerinin belirlenmesi botulismusun prognozuna faydalı olabileceği sonucuna varıldı.

Botulismuslu İneklerde Serum Haptoglobulin, Seruloplazmin ve Pseudokolinesteraz Seviyelerinin Değerlendirilmesi

The aim of this study was to determine serum pseudocholinesterase, rheumatoid factor, troponin I, C-reactive protein, caeruloplasmin, haptoglobin, urea, creatinin, creatinin kinase (CK), phosporus, calcium, alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in cows with or without botulism. The study included 15 holstein cows aged two to five years as the botulism group and control group consisting of 10 healthy cows. The group included both pregnant and dry period cattle. Serum concentration of all the parameters were measured using an autoanalyzer and Nefelometre equipment BNII. Mice inoculation test was performed to make diagnosis and Clostridium Botulinum type C and D toxins were determined in silage and blood that cows with botulism. Serum troponin I, C-reactive protein, rheumatoid factor, creatinine, creatinine kinase, phosphorus, calcium and AST did not differ significantly between two groups. Biochemistry analysis of serum showed that in the botulism group haptoglobin, caeruloplasmin and urea were higher and that pseudocholinesterase and alanine aminotransferase were lower than in the control group. Serum haptoglobin, caeruloplasmin, urea, alanine aminotransferase and especially pseudocholinesterase concentrations may prove beneficial to the prognosis of botulism.

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