Th anina Ghania AIT HAMOUDA, Nassima AIT ISSAD, Khaled ABDELOUAHED, Nora MIMOUNE, Salim BEKHOUCHE, Racha BOUBEUKER, Djilali DEGUI, Rachid KAIDI, Djamel KHELEF

Cezayir’in Kuzeydoğusundaki Koyun Plasentalarında Toxoplasma gondii’nin Moleküler Tespiti

Bu çalışmada, Toxoplasma gondii’nin B1 genini hedef alan direkt PCR ile koyun plasenta örneklerinde parazitik DNA’nın saptanması amaçlandı. Enfeksiyona ilişkin olası risk faktörleri de değerlendirildi. Cezayir’in kuzeydoğusundaki Tebessa bölgesinden 2019-2020 yılları arasında 23 çift likten toplam 307 dişi koyun örneklendi. Veriler, test edilen 307 dişi koyunun 114 (%37.1)’ünde T. gondii DNA’sının tespit edildiğini gösterdi. Çift likteki moleküler prevalans %82.6 olarak saptandı. Bir Al Ater (%32.4) ve Tlidjen’de (%43.6) pozitifl ik yüksek orandaydı. Yerleşik mod uygulayan çift liklerde kontaminasyon seviyesi yüksekti (%47.6); bu sistem, toksoplazmoz prevalansı üzerinde olumlu bir etkiye sahip olma eğilimi gösterdi (P

Molecular Detection of Toxoplasma gondii in Ewes Placenta in Northeastern Algeria

Th e present study aimed at the direct detection of parasitic DNA in placenta samples of ewes by PCR targeting the B1 gene of Toxoplasma gondii. We identified also the possible risk factors associated with the infection. A total of 307 female sheep from 23 farms were collected between 2019 and 2020 in the Tebessa region in northeastern Algeria. Data showed that T. gondii DNA was detected in 114 of the 307 tested females (37.1%). Th e on-farm molecular prevalence was 82.6%. High rates were revealed in Bir Al Ater (32.4%) and Tlidjen (43.6%). Th e level of contamination was high in farms applying a sedentary mode (47.6%); this system tends to have a positive eff ect on the prevalence of toxoplasmosis (P

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