Biljana DJURDJEVIĆ, Marko PAJIĆ, Slobodan KNEŽEVIĆ, Vladimir POLAČEK, Ksenija NEŠIĆ, Ivana VUČIĆEVIĆ, Dušica OSTOJIĆ ANDRIĆ

Aflatoksin B1 İle Enfekte Edilen Etlik Piliçlerde Yeni Kombine Adsorbanın Patolojik Lezyonların Oluşumuna Etkilerinin Değerlendirilmesi

Bu çalışma, daha önce in vitro olarak test edilen yeni bir birleşik adsorbanın (bentonit + maya hücre duvarı ekstraktı), 21 günlük yaşa kadar aflatoksin B1 ile kontamine edilmiş bir diyetle beslenen etlik piliçlerde patolojik lezyonların oluşumu üzerine koruyucu etkisinin araştırılması için yapıldı. Toplam 96 adet bir günlük Ross 308 hibrit etlik piliç, grup I (kontrol), grup II (5 g/kg adsorban), grup III (5 g/kg adsorban + 2 mg/kg aflatoksin B1) ve grup IV (2 mg/kg aflatoksin B1) olmak üzere dört gruba ayrıldı. Büyük ölçüde, AFB1 ve AFB1+adsorban verilmiş piliçlerde karaciğerde büyüme ve gevrek bir doku ve böbreklerde büyüme ve solgunluk görüldü. Dalağın boyutunda küçülme saptandı. Kontrol ve grup II’de açık patolojik değişiklikler gözlenmedi. Aflatoksin verilen gruplarda histopatolojik lezyonlar izlendi. Karaciğerde vakuolar hücre dejenerasyonu, mononükleer hücrelerin periportal ve perivasküler infiltrasyonu görüldü. Böbreklerde, hafif ile orta derecede kanamalar, tübüler epitel nekrozu ve heterofillerle birlikte mononükleer hücre infiltrasyonu saptandı. Grup IV’te duodenumda epitel hücrelerde deskuamasyon ile karakterize kataral enterit saptandı. Grup III’te daha hafif açık ve histopatolojik lezyonlar görüldü. Sonuç olarak, bu in vitro çalışma, 5 g/kg konsantreli yeni bir kombine toksin bağlayıcı ürünün eklenmesinin, etlik piliçlerde aflatoksikozisi tamamen iyileştirmediğini, ancak Avrupa’daki minimum %90 aflatoksin bağlama etkinliği için katı düzenleme şartlarını karşıladığını ortaya koydu.

Evaluation of the Eff ects of New Combined Adsorbent on Occurrence of Pathological Lesions in Afl atoxin B1‐Challenged Broiler Chickens

This experiment was performed to investigate the protective effect of a new combined adsorbent (bentonite + yeast cell wall extract) previously tested in vitro, on the occurrence of pathological lesions in broilers fed a diet contaminated with aflatoxin B1 until 21 days of age. A total of 96 one-day old Ross 308 hybrid broiler chickens were divided into four groups: group I (control), group II (5 g/kg adsorbent), group III (5 g/kg adsorbent + 2 mg/kg aflatoxin B1), and group IV (2 mg/kg aflatoxin B1). Grossly, AFB1 and AFB1+adsorbent fed birds showed enlargement, friable texture of liver and enlargement and pallorness of kidneys. Spleen revealed size reduction. No gross-pathological changes were observed in control and group II. Histopathologically, lesions were observed in aflatoxicated groups. Liver revealed vacuolar cell degeneration, periportal and perivascular infiltration of mononuclear cells. Kidneys revealed mild to moderate degree of haemorrhages, tubular epithelial necrosis and infiltration of mononuclear cells along with heterophils. In group IV, catarrhal enteritis characterized by desquamation of epithelial cells were found in the duodenum. A milder form of gross and histopathological lesions was seen in group III. In conclusion, the present study revealed that supplementation of a new combined toxin binder product in the concentration of 5 g/kg could not completely ameliorate aflatoxicity in broilers, although it met the stringent European regulation requirements for the minimum of 90% aflatoxin binding efficiency in in vitro study.

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