Ali KARADENİZ, Nejdet ŞİMŞEK, FERİDE KOÇ, HARUN ALP

Kronik flor toksikasyonu oluşturulan farelerde testis dokusundaki oksidatif hasar üzerine Panax ginseng'in koruyucu etkilerinin araştırılması

Bu araştırma kronik flor zehirlenmesinin fare testis dokusundaki zararlı etkilerine karşı Panax ginseng’in (PG) koruyucu özelliklerinin incelenmesi amacıyla yapılmıştır. Çalışmada toplam 40 adet yetişkin erkek Swiss albino fare her grupta 10 adet olacak şekilde rastgele 4 eşit gruba ayrıldı. Bu gruplar; kontrol (grup K, normal içme suyu), flor (grup F, 40 mg F/lt/gün flor), flor + Panax ginseng (grup F+PG, 40 mg F/lt/gün flor + 100 mg/kg/gün PG) ve Panax ginseng (grup PG, 100 mg/kg/gün PG) olarak ayrıldı. Otuz gün süren deney süresinin sonunda fareler servikal dislokasyonla öldürüldü. Testis dokusu malondialdehit (MDA) düzeyi, glutatyon (GSH), glutatyon peroksidaz (GSHPx) ve süperoksit dismutaz (SOD) aktiviteleri araştırıldı. Aynı zamanda testis dokusunda meydana gelen histolojik değişiklikler incelendi.Kontrol grubu ile karşılaştırıldığında grup F’deki farelerin testis dokusundaki MDA düzeylerinin arttığı, GSH, GSHPx ve SOD aktivitelerinin azaldığı (P

Anahtar Kelimeler:

Panax ginseng, antioksidanlar, flor, flor zehirlenmesi, doku bilimi, lipit peroksidasyonu, tıbbi bitkiler, fareler, koruyucu hekimlik, testis, toksisite

The investigation of protective effects of the Panax ginseng on oxidative damage ınduced by chronic fluoride toxicity in mice testis tissue

The aim of this study was to investigate the effects of Panax ginseng (PG) on antioxidant activity in testis tissue of healthy mice. In the study, totally 40 adult male Swiss albino mice were randomly divided into 4 equal groups; each group containing 10 mice. These groups were divided as control (group K, only drinking water), fluoride (group F, 40 mg F/lt/day flouride), fluoride + Panax ginseng (group F+PG, 40 mg F/lt/day fluoride +100 mg/kg/day PG) and Panax ginseng (group PG, 100 mg/kg/day PG). At the end of the experimental period (30 days), the mice were sacrifed by cervical dislocation. Lipid peroxidation (malondialdehyde, MDA), the activities of glutathione (GSH), glutathione-peroxidase (GSH-Px), superoxide dismutase (SOD) activities were investigated in testis tissues. Also were examined histological changes occured in testes tissue. In fluoride treated mice were observed significant increases in MDA levels and marked decreases in GSH, GSH-Px and SOD activities (p<0.05), and was determined damage of cell integrity in wall of tubuli seminiferous contorti and decrease to density of spermatozoon in epididymal lumen in compared to the control group. Panax ginseng treatment was significantly blocked the increases of MDA level and the decreases of GSH, GSH-Px and SOD activities (P<0.05). Also, the degenerative changes and the decreases of spermatozoon density induced by fluoride administration were ameliorated with PG treatments. As a result, fluoride may affect spermatogenesis by enhancing lipid peroxidation, and this injury can be decreased with PG treatment.

Keywords:

Panax ginseng, antioxidants, fluorine, fluorosis, histology, lipid peroxidation, medicinal plants, mice, preventive medicine, testes, toxicity,

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