Nejdet ŞİMŞEK, Ali KARADENİZ, ALEV GÜROL BAYRAKTAROĞLU

Ratlarda periferal kan hücreleri L-karnitin, arı sütü ve nar çekirdeğinin etkileri

Bu çalışma, ratlarda L-karnitin, arı sütü ve nar çekirdeğinin periferal kan hücreleri üzerine histometrik ve fizyolojik etkilerini araştırmak için yapıldı. Çalışmada kullanılan 24 Sprague Dawley rat, her grupta 6 rat olacak şekilde kontrol (K), L-karnitin (LK) (500 mg/ kg/gün, intraperitoneal, IP), arı sütü (AS, 300 mg/kg/gün, oral) ve nar çekirdeği (NÇ, 300 mg/kg/gün, oral) gruplarına ayrıldı. Eritrosit ve lökosit sayıları ile hemoglobin miktarı ve hematokrit değer hemositometrik yöntemlerle, T ve B lenfositlerinin yüzde oranı alfa naftil asetat esteraz (ANAE) boyamasıyla, eritrosit, T ve B lenfosit çapları ise Motic Image Plus 2.0 ML analiz paket programı aracılığı ile belirlendi. Kontrol grubuna göre LK, AS ve NÇ uygulanan gruplarda lökosit ve eritrosit sayısı ile hemoglobin ve hematokrit değerin daha fazla olduğu tespit edildi. Kontrol grubunda total lenfosit ve ANAE pozitif lenfosit oranları sırasıyla %73.4 ve %72.8 iken bu parametreler sırasıyla LK grubunda %78.55 ve %76.44, AS grubunda %68.35 ve %63.13, NÇ grubunda ise %66.52 ve %65.25 oranında hesaplandı. Ayrıca, histometrik ölçümler sonucunda lenfosit çaplarının K grubuna göre deney gruplarında azaldığı, eritrosit çaplarının ise özellikle AS ve NÇ gruplarında arttığı saptandı. Sonuç olarak, L-karnitinin immun sistem hücrelerini aktive edeci, arı sütü ve nar çekirdeğinin ise eritrosit sayısını ve çaplarını artırıcı özellikleri nedeniyle immun yetersizlik ve anemik hastalarda destekleyici antioksidan madde olarak kullanılabileceği kanaatine varıldı.

Anahtar Kelimeler:

tohum, sıçan, arı ürünleri, arı sütü, nar, kan hücreleri, alyuvarlar, akyuvarlar, T lenfositleri, bağışıklık uyarımı, hemoglobin, antioksidanlar, kansızlık

Effects of L-carnitine, royal jelly and pomegranate seed on peripheral blood cells in rats

This research was carry out to histometric and physiological effects of L-carnitine, royal jelly and pomegranate seed extract on blood cells in rats. Sprague Dawley rats were divided four groups, as each had 6 rats as follows: Control (C), L-carnitine (LC, 500 mg/kg/day, IP), royal jell (RJ, 500 mg/kg/day, orally), and pomegranate seed (PS, 500 mg/kg/day, orally). Red blood cell and white blood cell counts, and with haemoglobin amount and hematocrite values were determined by haemocytometric methods, and populations of B and T lymphocytes were counted by the alpha naphthyl acetate esterase (ANAE) staining method, and diameters of erythrocyte, B and T lymphocytes were determined by analysis program that the Motic Image Plus 2.0 ML. Erythrocyte and leukocyte counts, haemoglobin amount and hematocrite values were increased in of LC, RJ and PS received groups in compared with C group. The percentage rates of total lymphocyte and ANAE positive lymphocyte were determined 73.40% and 72.80% in C group, 78.55% and 76.44% in LC group, 68.35% and 63.13% in RJ group, and 66.52% and 65.25% in PS group, respectively. Also as a result of histometric measurement, lymphocyte diameter was decrease in experimental groups compared with C group, but erythrocyte diameter was increase in RJ and PS groups, especially. These results suggest that these components might be used a supportive agents in immune deficiency and anemic patients, because of the L-carnitine activated the immune cells, royal jelly and pomegranate seed increased the erythrocyte number and diameters.

Keywords:

seeds, rats, hive products, royal jelly, pomegranates, blood cells, erythrocytes, leukocytes, T lymphocytes, immunostimulation, haemoglobin, antioxidants, anaemia,

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