Jingbo 1 CHEN, Manjun 1 ZHAI, Heng YANG, ? Zongsheng 1 ZHAO, Cong YUAN, Yanping LIANG

Koyunlarda Mannoz Bağlayan Lektin (MBL) Polimorfizmi ve Mycoplasma ovipneumoniae Enfeksiyonu Sonrasında Serum MBL Protein Seviyeleri İle İlişkisi

Mannoz Bağlayan Lektin (MBL) karaciğer kaynaklı, dolaşımda yer alan plazma protein olup doğal bağışıklıkta önemli bir rol oynar. Koyunlarda MBL polimorfizmi Mycoplasma ovipneumoniae (MO) enfeksiyonu ile ilgilidir. Bu çalışmanın amacı 136 Çin Merinos koyununda farklı MBL genotipleri ile MO enfeksiyonuna karşı direnç arasındaki ilişkiyi araştırmaktır. MBL geninin ekzon-1 bölgesinin PCR amplikonlarının Tek Zincir Konformasyon Polimorfizm (SSCP) analizi BB, BC, CC ve DD olmak üzere dört şekil bulunduğunu tespit etti. BB, BC ve DD genotipleri sırasıyla 3 SNP, 2 SNP ve 4 SNP'ye sahipti. CC genotipi referans ile aynı bir sekansa sahip olup aralarında 3 sinonim SNP ve 6 sinonim olmayan SNP vardı. İstatistiksel analizler, Çin Merinos koyunlarında DD genotip MBL'nin serumda azalmış MBL protein seviyesi ile ilişkili olduğunu gösterdi. Bu tip bir genotipe sahip olan koyunlar MO enfeksiyonuna daha duyarlıyken CC genotipe sahip olanlarda tam tersine bir görüntü elde edildi. Bu durum MO enfeksiyonuna dirençli koyunların elde edilmesi için bir referans oluşturmaktadır.

Association Study Between Mannose-Binding Lectin (MBL) Polymorphisms and Serum MBL Protein Levels After Mycoplasma ovipneumoniae Infection in Sheep

Mannose Binding Lectin (MBL) is a liver-derived, circulating plasma protein that plays a pivotal role in innate immunity, however, MBL polymorphisms in sheep were associated with the incidence of MO infection. The objective of this study was to determine the relationships between the different genotypes of MBL and the resistance against MO infection disease, including three hundred and thirty-six individuals of the China Merino sheep. Single-Strand Conformation Polymorphism (SSCP) analyses of PCR amplicons of the exon-1 region of the MBL gene revealed four patterns: BB, BC, CC and DD. However, BB, BC and DD genotype have 3 SNPs, 2 SNPs, and 4 SNPs respectively, and CC genotype has only an identical sequence to the reference, among which there are 3 synonymous SNPs and 6 non-synonymous SNPs. Our statistic analysis showed that the DD genotype MBLs in China Merino sheep were associated with the decrease of MBL protein level in serum. The sheep carrying this kind of genotype were more susceptible to MO infection, however, an opposite result was found in sheep having CC genotype, which will provide a reference for molecular breeding of sheep breeds resistant to MO infection.

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