Investigation of Poly-?-Hydroxybutyrate (PHB) Production by Bacillus subtilis ATCC 6633 Under Diff erent Conditions [1]

Çalışmamızın amacı Bacillus subtilis ATCC 6633 suşunun farklı koşullar altında PHB verimliliğinin tayin edilmesidir. PHB miktarı 235 nm'ye ayarlanan UV spektrofotometre kullanılarak belirlenmiştir. İnkübasyon süresi, sıcaklık, pH, farklı karbon kaynakları, azot kaynakları ve karbon/azot oranının PHB sentezine etkisi araştırılmıştır. Çalışmanın sonuçları Bacillus subtilis ATCC 6633 suşunda en yüksek PHB birikiminin, 24 saat inkübasyonda (10.4981 ?g/ml), pH; 7'de (10.4981 ?g/ml), karbon kaynağı olarak D-mannitol içeren besiyerinde (23.6623 ?g/ml), azot kaynağı olarak L-glisin içeren besiyerinde (14.6217 ?g/ml), karbon/azot oranı 2.5 olan besiyerinde (3.2481 ?g/ml ) ve 30°C sıcaklıktaki kültür ortamında (10.4981 ?g/ml) elde edildiğini göstermiştir.

Farklı Koşullar Altında Bacillus subtilis ATCC 6633 Tarafından Poli-?-Hidroksibütirat (PHB) Üretiminin Araştırılması

The aim of our study was to assess the PHB productivity of Bacillus subtilis ATCC 6633 strain under diff erent conditions. PHB amount was measured by UV-VIS spectrophotometer at 235 nm. The eff ects of the incubation time, temperature, pH, carbon sources, nitrogen sources and the rate of carbon/nitrogen ratio on PHB production rate were investigated. The results demonstrated that the highest PHB production was obtained after 24 h of incubation (10.4981 ?g/ml), at 7.0 of pH (10.4981 ?g/ml), with D-mannitole (23.6623 ?g/ml) as carbon source, L-glycine (14.6217 ?g/ml) as nitrogen source, C/N ratio of 2.5 (3.2481 ?g/ml) and with a temperature of 30°C in culture media (10.4981 ?g/ml).

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