Staphylococcus aureus, Salmonella typhimurium ve Escherichia coli bilhassa halk sağlığı açısından önemli patojenlerdir ve gıda üretim işletmelerindeki proseslerde indikatör olarak değerlendirilmektedir. Bu çalışmada gıda üretim tesisleri yüzeylerinde kullanılan 4 adet ticari dezenfektan çeşidinin (klorin bazlı komponentler, alkol, Kuarterner Amonyum Bileşikleri (KAB) ve iyonik olmayan surfaktan) belirli süreler (1, 3, 5, ile 1, 3, 5, 10 ve 15 dakika) ile 3 farklı konsantrasyonda (%0.1, %0.5, %2) TSE EN 1276 süspansiyon test yöntemi ile etkinliği incelenmiştir. Buna göre KAB'nin S. aureus ve E. coli üzerinde çok etkili olduğu tespit edilmiştir. Ayrıca, klorlu bileşiklerin hem S. aureus hem de E. coli üzerinde, alkollü dezenfektanın ise tüm mikroorganizmalar üzerine etkili olduğu saptanmıştır (P
Staphylococcus aureus, Salmonella typhimurium and Escherichia coli are particularly important pathogens for public health and are considered as indicators in the processes used by the food production plants. In this study, four diff erent types of commercial disinfectants (chlorine-based compounds, alcohols, Quarterner Ammonium Compounds (QAC) and nonionic surfactants) used in food production facility at 3 concentrations (0.1%, 0.5%, 2%) were tested at specific periods (1, 3, 5 or 1, 3, 5, 10 and 15 min.) using the TSE EN 1276 Suspension Test Method. QACs were determined to be very eff ective on the S. aureus and E. coli. Additionally, chlorine-based compounds is also eff ective on S. aureus and E. coli and the alcoholic disinfectants are eff ective on all microorganisms that have been identified (P<=0.05).
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Wirtanen G, Aalto M, Härkönnen P, Gilbert P, Mattila-Sandholm T: Efficiacy testing of commercial disinfectants against foodborne pathogenic and spoilage microbes in biofilm-constructs. Eur Food Res Technol, 213, 409-414, 2001.
Wirtanen G, Salo S: Disinfection in food processing-efficacy testing of disinfectants. Rev Environ Sci Biotechnol, 2, 293-306, 2003.
Grönholm L, Wirtanen G, Ahlgren K, Nordström K, Sjöberg AM: Screening of antimicrobial activities of disinfectants and cleaning agents foodborne spoilage microbes. Z Lebensm Unters Forsch A, 208, 289-298, 1999.
Hayes PR: Food Microbiology and Hygiene. 2nd ed., pp. 344-369, Elsevier Applied Sciences, London. 1992.
Mcdonnell G, Russell A: Denver. Antiseptics and disinfectants: Activity, action, and resistance. Clin Microbiol Rev, 12, 147-179, 1999.
Troller JA: Sanitation in Food Processing. pp. 30-70, pp. 263-286, Academic Press, San Diego, 1983.
Merianos JJ: Surface-active agents. In, Block SS (Ed): Disinfection, Sterilization and Preservation. 5th ed., pp. 283-312, Lippincott Williams and Wilkins, Philadelphia, 2001.
Bloomfield SF, Arthur M, Looney E, Begun K, Patel H: Comparative testing of disinfectant and antiseptic products using proposed European suspension testing methods. Lett Appl Microbiol, 13, 233-237, 1991.
Forsythe SI, Hayes PR: Food Hygiene, Microbiology and HACCP. 3rd ed., pp. 327-371, An Aspen Publication, Gaithersburg, 1998.
TS EN 1276: Chemical disinfectants and antiseptics. Quantitative suspension test for the evaluation of bactericidal activity of chemical disinfectants and antiseptics used in food (phase 2, step 1). European Committee for Standardization (CEN), 1997 (In Turkish).
SPSS: SPSS Inc., SPSS Professional Statistics 7.5, Chicago, IL, USA, 1997.
Vatansever L, Gulmez M, Oral N, Guven A, Otlu S: Eff ects of Sumac (Rhus coriaria L.), oregano and lactic acid on microbiological decontamination and shelf-life of raw broiler drumsticks. Kafkas Univ Vet Fak Derg, 14, 211-216, 2008.
Senel, Y: The eff ect of some disinfectants used in food industry on microorganisms. MSc Thesis, Uludag University, Faculty of Agriculture, Bursa, 1999 (In Turkish).
Kasgar HS, Cotuk A: Investigation of eff ect of several disinfectants on the bacteria at several times. Biyoteknoloji (KÜKEM) Dergisi, 23 (Özel Sayı): 85-90, 1999 (In Turkish).
Kanai M, Koike Y, KuribaYoshi H, Miyazawa F: Bactericidal effect of evaporated ethanol. J Antibacterial Antifungal Agents, 22, 23-27,