EMEK DÜMEN, Ghassan ISSA, SERKAN İKİZ, ARZU FUNDA BAĞCIGİL, NACİYE YAKUT ÖZGÜR, TOLGA KAHRAMAN, SEVGİ ERGİN, Osman YEŞİL

Determining Existance and Antibiotic Susceptibility Status of Listeria monocytogenes Isolated from Dairy Products, Serological and Moleculer Typing of the Isolates [1]

Bu çalışmada İstanbul ve Trakya bölgelerinden toplanan 700 adet süt ve süt ürünleri örnekleri L. monocytogenes yönünden referans yöntemler ile analiz edilmiştir. Analiz edilen toplam 700 adet örnekten 20 adedi L. monocytogenes yönünden pozitif olarak saptanmıştır. Saptanan pozitif örneklere PCR analizi yapılmış ve örneklerin tamamı L. monocytogenes pozitif olarak doğrulanmştır. Pozitif örneklere antibiyotik duyarlılık testleri yapılarak etkenlerin toplam 16 adet antibiyotiğe karşı duyarlılık profilleri ortaya konmuştur. Daha sonra etkenlere serotiplendirme prosedürü uygulanmış ve etkenlerin 4d serotipine dahil oldukları belirlenmiştir. PCR prosedüründe doğrulanan örneklere DNA dizileme işlemi uygulanmış ve etkenlerin spesifik bölge DNA dizileri tespit edilmiştir. Sonraki aşamada ise etkenlerin Lineage (soy) grupları belirlenmiştir. Pozitif olarak tespit edilen etkenlerin tamamının Lineage I'e dahil olduğu saptanmıştır.

Süt ve Süt Ürünlerinde Listeria monocytogenes Varlığının Belirlenmesi, Antibiyotik Duyarlılık Profillerinin Saptanması, Izolatların Serolojik ve Moleküler Yöntemlerle Tiplendirilmesi

In this study, 700 samples of dairy and its products were collected from İstanbul and Trakya region and the samples were analyzed for L. monocytogenes by using the reference methods. 20 samples of 700 were L. monocytogenes positive. The positive samples were confirmed by using PCR. Antibiotic susceptibility tests were applied to the positive samples by using 16 diff erent kinds of antibiotic to expose the sensitivity profiles of the agents. Serotyping procedures were applied to the positive samples and all the agents were determined as L. monocytogenes serovar 4d. PCR confirmed samples were sequenced and specific DNA sequences of the agents were determined. Afterwards, Lineage groups of the positive samples were investigated and all the positive samples were exposed to be in Lineage I. The last step of our study was to compare our samples with the reference samples that are isolated from diff erent origins which were belonged to same and diff erent Lineages and to discuss all procedures that were applied in the study.

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