Prevalence of Bacillus cereus in Rabbit Meat Consumed in Burdur-Turkey, Its Enterotoxin Producing Ability and Antibiotic Susceptibility [1] [2]

Bu çalışma, tavşan etlerinde Bacillus cereus'un varlığı, enterotoksin üretme özelliği ve antibiyotik duyarlılığının belirlenmesi amacıyla yapıldı. B. cereus, egg yolk polymyxin agarda yüzeye ekim yöntemiyle sayıldı. Diarel tip enterotoksinin tespitinde BCET-RPLA testi kullanıldı. Antimikrobiyel duyarlılık testi için disk difüzyon metodu kullanıldı. B. cereus 50 tavşan eti örneğinin 18'inde (%36), ortalama 2.89x103 kob/g düzeyinde belirlendi. Toplam B. cereus izolatlarının 8 tanesi (%44.4) enterotoksin üretebilme özelliğinde bulundu. B. cereus izolatlarının %100'nün penisilin, %94.4'nün ampisilin, %27.7'sinin streptomisin, %22.2'sinin gentamisin ve eritromisine dirençli olduğu belirlenmesine karşın, kloramfenikol ve vankomisine direnç saptanmamıştır.

Burdur'da Tüketime Sunulan Tavşan Etlerinde Bacillus cereus Varlığı, Enterotoksin Üretme Yeteneği ve Antibiyotik Duyarlılığı

This study was undertaken to determine the existence, enterotoxin producing ability and antibiotic susceptibility of Bacillus cereus at rabbit meat. B. cereus was enumerated by the surface plating method with mannitol egg yolk polymyxin agar. The BCET-RPLA test was used for detecting of diarrheal type-enterotoxin. The disk diff usion method was used for antimicrobial sensitivity test. B. cereus was found in 18 (36%) of 50 samples of rabbit meat, with the mean contamination level of 2.89x103 cfu/g in positive samples. The 8 (44.4%) of the total isolates of B. cereus was able to produce enterotoxin. While antibiotic resistance of B. cereus isolates was found to be 100% penicillin, 94.4% ampicillin, 27.7% streptomycin, 22.2% gentamicin and erythromycin, no resistance was detected to chloramphenicol and vancomycin.

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