Handan Hilal ARSLAN, DEVRİM SARIPINAR AKSU, Semra ÖZDEMİR, OĞUZHAN YAVUZ, Mehmet Erman OR, Ümit Bora BARUTÇU

Evaluation of the Relationship of Blood Heavy Metal, Trace Element Levels and Antioxidative Metabolism in Cattle Which Are Living Near The Trunk Roads

Bu çalışmada, en az 3 yıldır anayol kenarında yaşayan sığırlarda bakır (Cu), çinko (Zn) ve demir (Fe) gibi bazı iz elementler, zehirli ağır metallerden olan kadmiyum (Cd) ve kurşun (Pb) ve antioksidan fonksiyonlar arasındaki olası ilişkinin tespit edilmesi amaçlandı. Çalışmada farklı ahırlarda yaşayan toplam 70 besi sığırı kullanıldı. Ana yola 0-500 metre mesafede yaşayan 54 (3-13 yaşlarında) sığır araştırma grubu olarak kullanıldı. Ana yola 2-3 km mesafede yaşayan 16 (3-12 yaşlarında) sığır ise kontrol grubunu oluşturdu. Hayvanlardan kan örnekleri toplandı ve Cd, Pb, Cu, Zn, Fe seviyeleri, lipid peroksidasyon göstergesi olan malondialdehid (MDA) ve antioksidasyon defans sistem indikatörleri olan eritrosit süperoksit dismutaz (SOD) aktivitesi, katalaz (CAT) aktivitesi ve plazma serüloplazmin (CP) seviyesi ölçüldü. Deneme grubu hayvanların Cd ve Pb seviyelerinin kontrol grubu hayvanlardan yüksek olduğu belirlendi (sırasıyla P

Anayol Yakınında Yaşayan Sığırlarda Kan Ağır Metal, İz Element Seviyeleri ve Antioksidan Metabolizma Arasındaki İlişkinin Değerlendirilmesi

In this study, detection of a possible relationship of some trace element levels of copper (Cu), zinc (Zn), and iron (Fe), toxic heavy metals, cadmium (Cd) and lead (Pb), and antioxidant functions in cattle, which are living near the trunk roads for at least 3 years, was aimed. Totally, 70 cattle, which were in diff erent herds, were used. Fifty-four cattle (3-13 years), which were living near the trunk roads at about 0-500 m were used for the experiment group. Also, 16 cattle (3-12 years), which were living far from trunk roads at about 2-3 km were used for the control groups. Blood samples were collected and Cd, Pb, Cu, Zn, Fe levels, lipid peroxidation product malondialdehyde (MDA) and antioxidative defense system indicators, erythrocyte superoxide dismutase (SOD) activity, catalase (CAT) activity, and plasma ceruloplasmin (CP) level were measured. Cd and Pb levels of the experimental animals were determined to be higher than the control group (P<0.01 and P<0.001, respectively). However, Zn level of the experiment group was found lower than the control animals (P<0.001). MDA increased about three times and CP increased about two times more than the control group (P<0.001). Significantly correlations were observed among Pb, MDA and CP levels. Also, important correlations were found between Fe and CP, also Cu and MDA levels. Besides, MDA and CAT levels of the animals increased with CP and SOD together, respectively. At the end of the study, Cd and Pb accumulation was observed in cattle, which were living near the road with intensive traffic for at least three years when compared with the control group. In addition, the results showed significant interactions among toxic heavy metals, physiological trace elements, enzymatic and non-enzymatic antioxidants and the lipid peroxidation.

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