ŞİNASİ AŞKAR, Fatma SAKARYA, MURAT YILDIRIM

The Potential Risk in Epizootiology of Bacterial Zoonozis: Pigeon (Columba livia domestica) Feces

Bu çalışmada, hobi amacıyla yetiştirilen evcil güvercinlerin bağırsak fl orasındaki bazı gram negatif bakteri profilini belirlemek ve izole edilen E. coli ile enterokok suşlarının bazı antibiyotiklere direncini disk difüzyon metotu ile araştırmak amaçlandı. Bu amaçla Kırıkkale ve çevresindeki 8 güvercin kümesinden toplam 215 adet güvercinden kloakal svap örneği alındı. 215 numunede 180 izolat elde edildi ve bu izolatların %46'sı E. coli , %10'u Enterobacter spp., %7'i Shigella spp., %4.5'i C. diversus, %3'ü K. ozoenae, %1.5'ü B. dispar, %1'i P. alcalifaciens, %1'ü S. rubidae ve %25', Enterococcus spp. olarak identifiye edildi. E. coli izolatlarının enrofl oksasin ve gentamisin karşı direnç taşımadığı, ampisilin-sulbaktam %70, nalidiksik asit %49, oksitetrasikline %64 dirençli olduğu tespit edildi. Ayrıca E. coli suşlarının %42.3'ünün, ?-laktamaz aktivitesi yönünden pozitif olduğu belirlendi.

Bakteriyel Zoonozların Epizootiyolojisinde Potansiyel Risk: Güvercin (Columba livia domestica) Dışkısı

The purposes of this study were to determine the profile of some Gram-negative bacteria in domestic pigeon intestinal microfl ora and isolated Enterococcus and E. coli strains to some antibiotics resistance were investigated by disc diff usion methods. For this purpose, cloacal swab samples were collected from a total of 215 pigeons from 8 diff erent pigeon-houses, in Kirikkale, Turkey. A total of 180 isolates were obtained from 215 samples and these isolates were identified as 46% E. coli, 10% Enterobacter spp., 7% Shigella spp., 4.5% C. diversus, 3% K. ozoenae, %1.5 B. dispar, 1% P. alcalifaciens, 1% S. rubidae and 25% Enterococcus spp. It was determined that the isolates of E. coli were not resistant to enrofl oxacine and gentamicine, while, they were found resistant to ampicilin-sulbactam, nalidixic acide and oxytetracyclin at the rates of 70%, 49% and 64% respectively. Moreover, 42.3% of the E. coli strains were identified as positive for ?-lactamase activities.

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Adesiyun AA, Downes M: Prevalence of antimicrobial resistance and enteropathogenic serogroups in Escherichia coli isolates from wildlife in Trinidad and Tobago. Vet Arhiv, 69, 335-347, 1999.

Arıkoğlu Ç, Aydın F: Kars Yöresinde evcil ve yabani hayvanlarda Campylobacter Jejuni'nin prevelansı. Kafkas Univ Vet Fak Derg, 3, 173-180, 1997.

Lundgren DL, Thorpe BD, Haskell CD: Infectious diseases in wild animals in Utah VI. experimental infection of birds with Rickettsia rickettsii. J Bacteriol, 91 (3): 963-966, 1966.

Facklam RR, Collins MD: Identification of enterococcus species isolated from human infections by a conventional test scheme. J Clin Microbiol, 27, 731-734, 1989.

Adesiyun AA, Seepersadsingh N, Inder L, Caesar K: Some bacterial enteropathogens in wildlife and racing pigeons from Trinidad. J Wild Dis, (1): 73-80, 1998.

Baele M, Devriese LA, Butaye P, Haesebrouck F: Composition of enterococcal and streptococcal flora from pigeon intestines. J Appl Microbiol, 92, 348-351, 2002.

Haag-Wackernagel D, Mochb H: Health hazards posed by feral pigeons. J Infect, 48 (4): 307-313, 2004.

Silva VL, Nicoli JR, Nascimento TC, Diniz CG: Diarrheagenic Escherichia coli strains recovered from urban pigeons (Columba livia) in Brazil and their antimicrobial susceptibility patterns. Curr Microbiol, 59, 308, 2009.

Ishiguro N, Oka C, Sato G: Isolation of citrate-positive variants of Escherichia coli from domestic pigeons, pigs, cattle, and horses. Appl Environ Microbiol, 36 (2): 217-222, 1978.

Tanaka C, Miyazawa T, Watarai M, Ishiguro N: Bacteriological survey of feces from feral pigeons in Japan. J Vet Med Sci, 67 (9): 951-953, 2005.

Kimpe A, Decostere A, Martel A, Haesebrouck F, Devriese LA: Prevalence of antimicrobial resistance among pigeon isolates of Streptococcus gallolyticus, Escherichia coli and Salmonella enterica sero- type Typhimurium. Avian Pathol, 31, 393-397, 2002.

Gülhan T, İlhan Z, Aksakal A, Solmaz H, Ekin IH: Hayvan orijinli Escherichia coli suşlarının enterotoksin tiplerinin (Lt, St) belirlenmesi. YYU Vet Fak Der, 20 (2): 27-31, 2009.

Bekar M: Enterobacteriaceae Familyası Mikroorganizmaların Genel Karakterleri ve Tanı Yöntemleri. s. 76-91, Kariyer matbaacılık Ltd. Şti., Ankara, 1997.

Koneman EW, Allen SD, Janda WM, Schreckenberger PC, Winn WC: Identification of Enterococcus species. In, Color Atlas and Texbook of Diagnostic Microbiology. 5th ed., pp. 616-621, Lippincott-Raven Publishers, Philadelphia, 1997.

Clinical Laboratory Standards Institute: Performance Standards for Antimicrobial Disk and Dilution Susceptibility Tests for Bacteria Isolated from Animals; Approved Standard-Second ed., M31-A2 and M37- A2. Pennsylvania, USA, 2002.

Kobayashi H, Pohjanvirta T, Pelkonen S: Prevalence and characteristics of intimin- and shiga toxin-producing Escherichia coli from gulls, pigeons and broilers in Finland. J Vet Med Sci, 64, 1071-1073,

Watt S, Lanotte P, Mereghetti L, Schouleur MM, Picard B, Quentin R: Escherichia coli strains from pregnant women and neonates: Intra- species genetic distribution and prevalence of virulence factors. J Clin Microbiol, 41 (5): 1929-1935, 2003.

Butaye P, Baele M, Devriese LA, Haesebrouck F: Comparison of susceptibility to antimicrobials of the enterococcal species isolated from pigeons (Columba livia). Microb Drug Resist, 8, 215-218, 2002.

Fisher JF, Meroueh SO, Mobashery S: Bacterial resistance to beta- lactam antibiotics: Compelling opportunism, compelling opportunity. Chem Rev, 105, 395-424, 2005.

Thomson JM, Bonomo RA: The threat of antibiotic resistance in gram-negative pathogenic bacteria: Beta-lactams in peril. Curr Opin Microbiol, 8 (5): 518-524, 2005.