Mehmet KALE, Dilek OZTURK, Faruk PEHLIVANOGLU, Ahmet Ali TOK, Yuksel GULDALI, Hulya TURUTOGLU

In vitro diagnosis of bovine tuberculosis by $gamma$-interferon assay

Bu çalışmanın amacı, sığır tüberkülozunun teşhisinde $gamma$-IFN testinin sensitivite ve spesifitesini belirlemek ve $gamma$-IFN testi ile intradermal tüberkülin testinin sonuçlarını karşılaştırmaktır. Tüberküloz şüpheli 6 sürüden toplam 126 sığırdan defibrine kan örnekleri toplandı ve in vitro $gamma$-IFN testi uygulandı. Aynı zamanda, aynı hayvanlara intradermal tüberkülin testi yapıldı. $gamma$-IFN testi ile sığırların 62’si (%49.2) pozitif bulunurken, intradermal tüberkülin testi ile 67 (%53.2) sığırın pozitif olduğu belirlendi. $gamma$IFN testinin sensitivite ve spesifitesi, sırasıyla %90 ve %97 bulundu. Sonuç olarak, sığır tüberkülozunun teşhisinde $gamma$-IFN testinin, uygulaması kolay, kısa sürede sonuç veren bir test olduğu ve sığır tüberkülozunun eradikasyon proğramlarında intradermal tüberkülin testine alternatif olabileceği düşünüldü.

$gamma$-interferon testi ile sığır tüberkülozunun in vitro teşhisi

The purpose of this study was to determine the sensitivity and specificity of the $gamma$-IFN assay and to compare the $gamma$-IFN assay and intradermal tuberculin test results for detection of bovine tuberculosis. Defibrined blood samples were collected from 126 cattle in 6 herds suspected with tuberculosis and tested by in vitro $gamma$-IFN assay. Intradermal tuberculin test was also performed on the same animals. While 62 cattle (49.2%) were found positive by $gamma$-IFN assay, 67 cattle (53.2%) were detected positive by intradermal tuberculin test. The sensitivity and specificity of $gamma$-IFN assay was estimated to be 90% and 97%, respectively. In conclusion, it is thought that $gamma$-IFN assay is easier to perform and it shortens the time for diagnosis of bovine tuberculosis and it can be an alternative test to intradermal tuberculin test in bovine tuberculosis eradication programs.

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1. Cagiola M, Feliziani F, Severi G, Pasquali P, Rutuli D: Analysis of possible factors affecting the specity of the gamma interferon test in tuberculosis-free cattle herds. Clin Diagn Lab Immunol, 11, 952-956, 2004.
2.Pollock JM, Welsh MD, McNair J: Immune responses in bovine tuberculosis: Towards new strategies for the diagnosis and control of disease. Vet Immunol Immunopathol, 108, 3743, 2005.
3.Whipple DL, Palmer MV, Slaughter RE, Jones SL: Comparison of purified protein derivates and effect of skin testing on results of a commercial gamma interferon assay for diagnosis of tuberculosis in cattle. J Vet Diagn Invest, 13, 117122, 2001.
4.Lilenbaum W, Ribemro ER, Souza GN, Moremra EC, Fonseca LS, Ferremra MAS, Schettmnm J: Evaluation of an ELISA-PPD for the diagnosis of bovine tuberculosis in field trials in Brazil. Res Vet Sci, 66, 191-195, 1999.
5.Wood PR, Rothel JS: In vitro immunodiagnostic assays for bovine tuberculosis. Vet Microbiol, 40, 125-135, 1994.
6.Palmer MV, Waters WR, Thacker TC, Greenwald R, Esfandiari J, Lyashchenko KP: Effects of different tuberculin skin-testing regimens on gamma interferon and antibody responses in cattle experimentally infected with Mycobacterium bovis. Clin Vaccine Immunol, 13, 387-394, 2006.
7.Rua Domenech R, Goodchild AT, Vordermeier HM, Hewinson RG, Christiansen KH, Clifton-Hadley RS: Ante mortem diagnosis of tuberculosis in cattle: A review of the tuberculin tests, $gamma$-interferon assay and ancillary diagnostic techniques. Res Vet Sci, 81, 190-210, 2006.
8.Gormley E, Doyle MB, McGill K, Costello E, Good M, Collins JD: The effect of the tuberculin test and the consequences of a delay in blood culture on the sensitivity of a gamma-interferon assay for the detection of Mycobacterium bovis infection in cattle. Vet Immunol Immunopathol, 102, 413-420, 2004.
9.Ryan TJ, Buddle BM, de Lisle GW: An evaluation of the gamma interferon test for detecting bovine tuberculosis in cattle 8 to 28 days after tuberculin skin testing. Res Vet Sci, 69, 57-61, 2000.
10.Gormley E, Doyle MB, Fitzsimons T, McGill K, Collins JD: Diagnosis of Mycobacterium bovis infection in cattle by use of the gamma-interferon (Bovigam®) assay. Vet Microbiol, 112, 171-179, 2006.
11.Wood PR, Corner LA, Plackett P: Development of a simple, rapid, in vitro cellular assay for bovine tuberculosis is based on the production of $gamma$-interferon. Res Vet Sci, 49, 4649, 1990.
12. Gonzales-Llamazares OR, Gutierrez CB, Alvarez Nistal D, de la Puente R, Dominguez Rodriguez L, Rodriguez Ferri F: Field evaluation of the single intradermal cervical tuberculin test and the interferon-γ assay for detection and eradication of bovine tuberculosis in Spain. Vet Microbiol, 70, 55-66, 1999.
13.Wood PR, Corner LA, Rothel JS, Baldock C, Jones SL, Cousins DB, McCormick BS, Francis BR, Creeper J, Tweedle NE: Field comparison of the interferon gamma assay and the intradermal tuberculin test for the diagnosis of bovine tuberculosis. Aust Vet J, 68, 286-290, 1991.
14.Gumussoy KS, Atasever A, Aydmn F, Ozcan M, Beyaz L, Hmzlmsoy H, Abay S: Prevalence of tuberculosis in Turkey. Med Weter, 63, 305-308, 2007.
15.Ozmen O, Kurşun O, Ozcelik M: Bovine tuberculosis in Burdur, southern Turkey: Epidemiological, pathological and economic study. Int J Tuberc Lung Dis, 9, 1398-1402, 2005.
16.Unver A, Atabay HI, Günes V, Citil M, Erdogan HM: Kars yöresinde slğlr tüberkülozunun yayglnllğlnln PCR yöntemi ile belirlenmesi. Kafkas Univ Vet Fak Derg, 13, 27-31, 2007.
17.Solmaz H, Ilhan Z, Aksakal A, Gulhan T, Ekin IH: Detection of bovine tuberculosis by tuberculin test and polymerase chain reaction in Van, Turkey. Turk J Vet Anim Sci, 33, 229-233.