İshalli neonatal kuzularda enterik patojenlerin belirlenmesi
Bu çalışmada Kars yöresinde tesedüfi örnekleme ile seçilen 4 koyunculuk işletmesinde neonatal (0-28 gün) kuzu ishallerinin etiyolojisinde bazı patojenlerin varlığı araştırıldı. Bu amaçla 2006 doğum sezonunda yapılan günlük ziyaretlerle neonatal kuzularda belirlenen ishal olgularında dışkı örnekleri toplanarak Escherichia coli F5, E. coli O157, Cryptosporidium parvum, rotavirus, coronavirus, Clostridium perfringens spp., Salmonella spp. ve Campylobacter spp. yönünden değerlendirildi. Dışkı örneklerinden yapılan mikrobiyolojik analizlerde Campylobacter spp. belirlenmezken sadece 1 olguda Salmonella spp. izole ve identifiye edildi. ELISA tekniği ile ishalli kuzuların dışkı örneklerinden E. coli F5 %10.5 (4/38), E. coli O157 %10.9 (12/110), C. parvum %21.05 (8/38), rotavirus %5.3 (3/56) ve coronavirus %21.4 (12/56) oranlarında belirlendi. Septisemiden ölen kuzulardan alınan doku örneklerinde E. coli %42.5 (9/21) oranında izole edildi. İshal olgularında Cl. perfringens ve toksinlerinin rolünü belirlemek amacıyla, toplam 56 ishalli dışkı örneği ELISA tekniği ile incelendi. Dışkı örneklerinin %57 (32/56)’sinin Cl. perfringens α, β ve € toksinlerinin en az biri yönünden pozitif olduğu belirlendi. Pozitif bulunan örneklerin biyotiplendirmesinde Cl. perfringens tip A %18.8 (6/32), Cl. perfringens tip B %21.9 (7/32), Cl. perfringens tip C %28.2 (9/32) ve Cl. perfringens tip D %31.3 (10/32) oranında belirlendi. Sonuç olarak, neonatal kuzu ishallerinin etiyolojisinde Cl. perfringens, C. parvum, E. coli F5, rotavirus ve coranavirusun birlikte veya tek başına bulunduğu ve salgınlara neden olarak, yüksek oranda morbidite ve mortalite ile seyrettiği belirlendi. .
Enteric pathogens in the aetiology of diarrhoea in neonatal lambs
This study determined some enteropathogens in diarrhoeic neonatal lambs (0-28 days old) in randomly selected 4 sheep farms in Kars. Feacal samples were collected from diarrhoeic cases on daily visits during 2006 lambing season in order to determine Escherichia coli F5, E. coli O157, Cryptosporidium parvum, rotavirus, coronavirus, Clostridium perfringens, Salmonella spp. and Campylobacter spp. Microbiological examination of faecal samples revealed absence of Campylobacter spp. and Salmonella spp. in one case. E. coli F5 10.5% (4/38), E. coli O157 10.9% (12/110), C. parvum 21.05% (8/38), rotavirus 5.3% (3/56) and coronavirus 21.4% (12/56) were also determined in samples by using ELISA. Analyses of tissue samples taken from lambs died of septicaemia revelaed presence of E. coli (42.5%, 9/21). Cl. perfringens and their toxins were determined in feaces of 56 diarrhoeic lambs using ELISA. Of these, 57% (32/56) were positive for Cl. perfringens and at least one of these cases had α, β and € toxins. Biotyping of these positive samples revelaed Cl. perfringens type A (18.8%, 6/32), Cl. perfringens type B (21.9%, 7/32), Cl. perfringens type C (28.2%, 9/32) and Cl. perfringens type D (31.3%, 10/32). The results of this study disclosed that Cl. perfringens, C. parvum, E. coli F5, rotavirus and coronavirus played role, either alone or togather, in the aetiology of neonatal lamb diarrhoae and that may cause outbreaks leading to higher morbidity and mortality rates.
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