Ercan KOCA, Nefise AKKOÇ, PINAR ŞANLIBABA, MUSTAFA AKÇELİK

Nisin dirençlilik ve regülasyon genlerinin Laktisin 481 üreticisi L. lactis subsp. lactis MBLL9 suşunda anlatımı

Bu çalışmada, nisin dirençlilik (nisFEG ve nisI), regülasyon (nisRK) ve regülasyon/dirençlilik (nisRKFEG) genlerinin laktisin 481 üreticisi Lactococcus lactis subsp. lactis MBLL9 suşundaki ifadesi araştırıldı. nisFEG (LL175), nisI (LL176), nisRK (LL177), nisRKFEG (LL178) genlerini ifade eden rekombinant suşların ve kontrol suşun (MBLL9) laktisin 481 üretiminin, sırasıyla 80.000 AU $L^ {-1}$, 80.000 AU $L^ {-1}$, 40.000 AU $L^ {-1}$, 40.000 AU $L^ {-1}$ ve 80.000 AU $L^ {-1}$ düzeylerinde olduğu tespit edildi. Rekombinant suşlarda nisin dirençliliğin, LL175, LL176 ve LL178 suşlarında sırasıyla 300 IU $mL^ {-1}$, 350 IU $mL^ {-1}$ ve 500 IU $mL^ {-1}$ düzeylerine yükseldiği, LL177 suşunda ise 100 IU $mL^ {-1}$ düzeyine düştüğü saptandı.

Anahtar Kelimeler:

Lactococcus lactis, genler, soy, regülasyon genleri, rekombinant protein, nisin, Lactococcus lactis subsp. lactis, ilaç direnci, gen ifadesi

Expression of nisin resistance and regulation genes in Lacticin 481 producer strain L. lactis subsp. lactis MBLL9

In this study, expression of nisin resistance (nisFEG ve nisI), regulation (nisRK) and regulation/resistance (nisRKFEG) genes in lacticin 481 producer strain Lactococcus lactis subsp. lactis MBLL9, was investigated. Lacticin 481 production levels for recombinant strains expressing nisFEG (LL175), nisI (LL176), nisRK (LL177), nisRKFEG (LL178) genes and control strain (MBLL9) were determined as 80.000 AU $L^ {-1}$, 80.000 AU $L^ {-1}$, 40.000 AU $L^ {-1}$, 40.000 AU $L^ {-1}$ and 80.000 AU $L^ {-1}$, respectively. Nisin resistance levels in recombinant LL175, LL176 and LL178 strains were increased to 300 IU $mL^ {-1}$, 350 IU $mL^ {-1}$ and 500 IU mL1levels, respectively, however it was decreased to 100 IU $mL^ {-1}$ in recombinant strain LL177.

Keywords:

Lactococcus lactis, genes, strains, regulatory genes, recombinant proteins, nisin, Lactococcus lactis subsp. lactis, drug resistance, gene expression,

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