DİLEK ÖZTÜRK, Faruk PEHLIVANOGLU, MEHMET KALE, Ahmet Ali TOK, Yuksel GULDALI, Hülya TÜRÜTOĞLU

In vitro diagnosis of bovine tuberculosis by $\gamma$-interferon assay

Bu çalışmanın amacı, sığır tüberkülozunun teşhisinde $\gamma$-IFN testinin sensitivite ve spesifitesini belirlemek ve $\gamma$-IFN testi ile intradermal tüberkülin testinin sonuçlarını karşılaştırmaktır. Tüberküloz şüpheli 6 sürüden toplam 126 sığırdan defibrine kan örnekleri toplandı ve in vitro $\gamma$-IFN testi uygulandı. Aynı zamanda, aynı hayvanlara intradermal tüberkülin testi yapıldı. $\gamma$-IFN testi ile sığırların 62’si (%49.2) pozitif bulunurken, intradermal tüberkülin testi ile 67 (%53.2) sığırın pozitif olduğu belirlendi. $\gamma$IFN testinin sensitivite ve spesifitesi, sırasıyla %90 ve %97 bulundu. Sonuç olarak, sığır tüberkülozunun teşhisinde $\gamma$-IFN testinin, uygulaması kolay, kısa sürede sonuç veren bir test olduğu ve sığır tüberkülozunun eradikasyon proğramlarında intradermal tüberkülin testine alternatif olabileceği düşünüldü.

Anahtar Kelimeler:

Mycobacterium tuberculosis, sığır, teşhis, tanı teknikleri, hastalık kontrolü, hastalık önleme, interferon, deri testleri, tüberküloz

$\gamma$-interferon testi ile sığır tüberkülozunun in vitro teşhisi

The purpose of this study was to determine the sensitivity and specificity of the $\gamma$-IFN assay and to compare the $\gamma$-IFN assay and intradermal tuberculin test results for detection of bovine tuberculosis. Defibrined blood samples were collected from 126 cattle in 6 herds suspected with tuberculosis and tested by in vitro $\gamma$-IFN assay. Intradermal tuberculin test was also performed on the same animals. While 62 cattle (49.2%) were found positive by $\gamma$-IFN assay, 67 cattle (53.2%) were detected positive by intradermal tuberculin test. The sensitivity and specificity of $\gamma$-IFN assay was estimated to be 90% and 97%, respectively. In conclusion, it is thought that $\gamma$-IFN assay is easier to perform and it shortens the time for diagnosis of bovine tuberculosis and it can be an alternative test to intradermal tuberculin test in bovine tuberculosis eradication programs.

Keywords:

Mycobacterium tuberculosis, cattle, diagnosis, diagnostic techniques, disease control, disease prevention, interferon, skin tests, tuberculosis,

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