Ibrahim ELDESOUKY, Nayel MOHAMED, Doaa KHALAF, Akram SALAMA, Ahmed ELSIFY, Rabee OMBARAK, Salah EL-BALLAL, Mohamed EFFAT, Mona SHABRAWY AL

Candida Mastitis in Dairy Cattle with Molecular Detection of Candida albicans

Bu çalışmada Candida albicans'ın moleküler yöntemle belirlenmesi suretiyle sütçü inek mastitlerinde Candida türlerinin prevalansının belirlenmesi amaçlanmıştır. Klinik olarak mastitis semptomları gösteren toplam 150 adet sütçü ineğe ait süt örnekleri toplandı. C. albicans'ın moleküler yöntemle belirlenmesinin yanında organizmaların fenotipik ve değişik kültürlerdeki fizyolojik kriterleri baz alınarak izolasyon ve identifikasyonu yapıldı. Candida türlerinin 41 izolatı %27.3 prevalans oranı ile elde edildi. C. albicans en çok belirlenen tür olarak kaydedildi (%29.3). Fenotipik olarak C. albicans olduğu belirlenen 12 adetten 8'i 26S rRNA geni için spesifik primer çifti kullanılarak yapılan PCR ile onaylandı. Moleküler yöntemle C. albicans olduğu belirlenen tüm izolatlarda spesifik virulans olarak Phospholipase B1 geni tespit edildi. Bu çalışma ile Candida mastitislerinin prevalansı belirlenmiş ve C. albicans kaynaklı mikotik mastitislerde diagnostik moleküler yöntemin kullanılabilirliği gösterilmiştir.

Candida albicans'ın Moleküler Tespiti İle Sütçü İneklerde Candida Mastitisi

This study focused on determining the prevalence of Candida species involved in dairy cattle mastitis with molecular detection of Candida albicans. A total of 150 milk samples were collected from dairy cattle showing clinical mastitis. Isolation and identification of organisms through phenotypical and physiological criteria on different media were performed as well as molecular identification of C. albicans. Fourty one isolates of Candida species were recovered with a prevalence of 27.3%. C. albicans was the dominating species (29.3%). Out of 12 strains phenotypically identified as C. albicans, 8 were confirmed by PCR using species specific primer for the 26S rRNA gene of C. albicans. A specific virulence determinant Phospholipase B1 gene was detected in all molecularly identified C. albicans isolates. This study has clearly shown the prevalence of Candida mastitis and providing a new attractive diagnostic molecular tool for mycotic mastitis caused by C. albicans.

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