Wenyan GAI, Junwei WANG, Juan WANG, Zhigang CUI, Zhina QU, Jun HONG, Jinghua CUI, Xiaoli DU, Xiumei HUANG, Xumin CAO, Jianmei ZHAO

Characterization and Distribution of Salmonella spp. Isolated from Poultry Slaughterhouse-processingin Shandong Province [1]

Salmonella spp. dünya çapında kanatlı hayvan etlerinden köken alan halk sağlığı bakımından en önemli gıda kaynaklı patojendir. Bu çalışmanın amacı Çin'de 12 değişik bölgede yer alan kesimhanelerde kesilen kanatlı hayvanlara ait ürünlerde Salmonella spp. prevalansını tespit etmek ve tüm izolatları serotipleri, PFGE ve MLST görüntüleri ile antimikrobiyal duyarlılıklarına göre karakterize etmektir. Çalışmada Salmonella spp. prevalansı kanatlı kesimhanelerinde %10.4 olarak belirlendi. 13 serotipi içeren 100 Salmonella suşu tespit edildi. Örneklerden en çok izole edilen etken S. Enteritidis olarak kaydedildi. İzolatlar belirtilen antibiyotiklere direnç gösterdi; sulfisozaksol (SF) (%79.0), doksisilin (DOX) (%68.0), tetrasiklin (TE) (%65.0), florfenikol (FFC) (%64.0), ampisillin (AM) (%50.0), gentamisin (GM) (%48.0), trimethoprim-sulfamethoksazol (SXT) (%30.0), spektinomisin (SPT) (%30.0), enrofloksasin (ENR) (%10.0), ofloksasin (NOR) (%10.0), amoksisilin potasyum klavulanat (AC) (%1.0) ve polimiksin (PME) (%4.0). Salmonella spp. izolatları cefotaxime (EFT) karşı dirençli değildi. Her bir izolat en az 3 grup antimikrobiyal madde olmak üzere çoklu ilaç direnci gösterdi. PFGE ile elde edilen dört küme ve 38 parmakizi-görüntüsü değişik bölgelerden toplanan suçların arasından belirlendi. Suşlar arası asosiasyon ve bazı bölgelerde persiste suşların varlığı tespit edildi. İzolatların MLST analizi 10 ST belirledi. Yedi housekeeping gen farklı varyasyonlara sahipti.

Shandong Eyaletindeki Kanatlı Hayvan Kesimhanelerinden İzole Edilen Salmonella spp.'nin Karakterizasyonu ve Yaygınlığı

Salmonella spp.is the most important food-borne pathogens of public health interest incriminated in poultry meat worldwide. The purpose of this study to estimate the prevalence Salmonella spp. contamination in poultry products from 12 different located geographical areas from among the 12 poultry slaughterhouses authorized in China and to characterize all the isolates by serotypes, PFGE patterns, MLST patterns and antimicrobial susceptibility. The prevalence of Salmonella spp. in the poultry slaughterhouse was 10.4%. All 100 strains of Salmonella spp. comprising 13 majority serotypes were identified. S. Enteritidis was the most frequently isolated from samples. The isolates displayed resistance to sulfisoxazole (SF) (79.0%), doxycycline (DOX) (68.0%), tetracycline (TE) (65.0%), florfenicol (FFC) (64.0%), ampicillin (AM) (50.0%), gentamicin (GM) (48.0%), trimethoprim-sulfamethoxazole (SXT) (30.0%), spectinomycin (SPT) (30.0%), enrofloxacin (ENR) (10.0%), ofloxacin (NOR) (10.0%), amoxicillin potassium clavulanat (AC) (1.0%) and polymyxin (PME) (4.0%). The Salmonella spp. isolates were not resistant to cefotaxime (EFT). Each isolates were multi-drug resistant as they were resistant to at least 2 groups of antimicrobials. Four clusters and 38 fingerprint-patterns generated by PFGE were identified among strains recovered from various locations, providing information on associations among the strains as well as evidence of the existence of persistent strains in some areas. MLST analysis of isolates identified the 10 STs, the 7 housekeeping genes had the different variation.

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